| Summary: | Medicago sativa ‘Deqin’ is an excellent alfalfa landrace with strong drought and heat resistant which can grow and propagate very well in Deqin, a xerothermic valley of Jinsha River, China. In this study, the complete chloroplast genome of M. sativa ‘Deqin’ was assembled. The complete chloroplast genome of M. sativa ‘Deqin’ represents a typical circular with 125,470 bp in length, containing one inverted repeat (IR) region. Gene prediction revealed 110 genes encoding 76 proteins, 30 transfer RNAs, and four ribosome RNAs. Three genes (rps16, rpl22 and infA) are absent. The overall GC content is 33.9%. The phylogenetic analysis revealed that M. sativa ‘Deqin’ belonged to the IR lacking clade, and was closely related to M. sativa with a 100% bootstrap support.
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