Complete blood count reference intervals from a healthy adult urban population in Kenya.
BACKGROUND:There are racial, ethnic and geographical differences in complete blood count (CBC) reference intervals (RIs) and therefore it is necessary to establish RIs that are population specific. Several studies have been carried out in Africa to derive CBC RIs but many were not conducted with the...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5991659?pdf=render |
| _version_ | 1819045676378488832 |
|---|---|
| author | Geoffrey Omuse Daniel Maina Jane Mwangi Caroline Wambua Kiran Radia Alice Kanyua Elizabeth Kagotho Mariza Hoffman Peter Ojwang Zul Premji Kiyoshi Ichihara Rajiv Erasmus |
| author_facet | Geoffrey Omuse Daniel Maina Jane Mwangi Caroline Wambua Kiran Radia Alice Kanyua Elizabeth Kagotho Mariza Hoffman Peter Ojwang Zul Premji Kiyoshi Ichihara Rajiv Erasmus |
| author_sort | Geoffrey Omuse |
| collection | DOAJ |
| description | BACKGROUND:There are racial, ethnic and geographical differences in complete blood count (CBC) reference intervals (RIs) and therefore it is necessary to establish RIs that are population specific. Several studies have been carried out in Africa to derive CBC RIs but many were not conducted with the rigor recommended for RI studies hence limiting the adoption and generalizability of the results. METHOD:By use of a Beckman Coulter ACT 5 DIFF CP analyser, we measured CBC parameters in samples collected from 528 healthy black African volunteers in a largely urban population. The latent abnormal values exclusion (LAVE) method was used for secondary exclusion of individuals who may have had sub-clinical diseases. The RIs were derived by both parametric and non-parametric methods with and without LAVE for comparative purposes. RESULTS:Haemoglobin (Hb) levels were lower while platelet counts were higher in females across the 4 age stratifications. The lower limits for Hb and red blood cell parameters significantly increased after applying the LAVE method which eliminated individuals with latent anemia and inflammation. We adopted RIs by parametric method because 90% confidence intervals of the RI limits were invariably narrower than those by the non-parametric method. The male and female RIs for Hb after applying the LAVE method were 14.5-18.7 g/dL and 12.0-16.5 g/dL respectively while the platelet count RIs were 133-356 and 152-443 x10(3) per μL respectively. CONCLUSION:Consistent with other studies from Sub-Saharan Africa, Hb and neutrophil counts were lower than Caucasian values. Our finding of higher Hb and lower eosinophil counts compared to other studies conducted in rural Kenya most likely reflects the strict recruitment criteria and healthier reference population after secondary exclusion of individuals with possible sub-clinical diseases. |
| first_indexed | 2024-12-21T10:32:21Z |
| format | Article |
| id | doaj.art-0c2cc98f2e4e4dfb8fc8ef01f39b7b3e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T10:32:21Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0c2cc98f2e4e4dfb8fc8ef01f39b7b3e2022-12-21T19:07:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019844410.1371/journal.pone.0198444Complete blood count reference intervals from a healthy adult urban population in Kenya.Geoffrey OmuseDaniel MainaJane MwangiCaroline WambuaKiran RadiaAlice KanyuaElizabeth KagothoMariza HoffmanPeter OjwangZul PremjiKiyoshi IchiharaRajiv ErasmusBACKGROUND:There are racial, ethnic and geographical differences in complete blood count (CBC) reference intervals (RIs) and therefore it is necessary to establish RIs that are population specific. Several studies have been carried out in Africa to derive CBC RIs but many were not conducted with the rigor recommended for RI studies hence limiting the adoption and generalizability of the results. METHOD:By use of a Beckman Coulter ACT 5 DIFF CP analyser, we measured CBC parameters in samples collected from 528 healthy black African volunteers in a largely urban population. The latent abnormal values exclusion (LAVE) method was used for secondary exclusion of individuals who may have had sub-clinical diseases. The RIs were derived by both parametric and non-parametric methods with and without LAVE for comparative purposes. RESULTS:Haemoglobin (Hb) levels were lower while platelet counts were higher in females across the 4 age stratifications. The lower limits for Hb and red blood cell parameters significantly increased after applying the LAVE method which eliminated individuals with latent anemia and inflammation. We adopted RIs by parametric method because 90% confidence intervals of the RI limits were invariably narrower than those by the non-parametric method. The male and female RIs for Hb after applying the LAVE method were 14.5-18.7 g/dL and 12.0-16.5 g/dL respectively while the platelet count RIs were 133-356 and 152-443 x10(3) per μL respectively. CONCLUSION:Consistent with other studies from Sub-Saharan Africa, Hb and neutrophil counts were lower than Caucasian values. Our finding of higher Hb and lower eosinophil counts compared to other studies conducted in rural Kenya most likely reflects the strict recruitment criteria and healthier reference population after secondary exclusion of individuals with possible sub-clinical diseases.http://europepmc.org/articles/PMC5991659?pdf=render |
| spellingShingle | Geoffrey Omuse Daniel Maina Jane Mwangi Caroline Wambua Kiran Radia Alice Kanyua Elizabeth Kagotho Mariza Hoffman Peter Ojwang Zul Premji Kiyoshi Ichihara Rajiv Erasmus Complete blood count reference intervals from a healthy adult urban population in Kenya. PLoS ONE |
| title | Complete blood count reference intervals from a healthy adult urban population in Kenya. |
| title_full | Complete blood count reference intervals from a healthy adult urban population in Kenya. |
| title_fullStr | Complete blood count reference intervals from a healthy adult urban population in Kenya. |
| title_full_unstemmed | Complete blood count reference intervals from a healthy adult urban population in Kenya. |
| title_short | Complete blood count reference intervals from a healthy adult urban population in Kenya. |
| title_sort | complete blood count reference intervals from a healthy adult urban population in kenya |
| url | http://europepmc.org/articles/PMC5991659?pdf=render |
| work_keys_str_mv | AT geoffreyomuse completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT danielmaina completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT janemwangi completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT carolinewambua completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT kiranradia completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT alicekanyua completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT elizabethkagotho completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT marizahoffman completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT peterojwang completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT zulpremji completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT kiyoshiichihara completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya AT rajiverasmus completebloodcountreferenceintervalsfromahealthyadulturbanpopulationinkenya |