Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and Optimization
One of the leading limiting factors for wider industrial production and commercialization of microbial biopesticides refers to the high costs of cultivation media. The selection of alternative sources of macronutrients crucial for the growth and metabolic activity of the producing microorganism is a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1165 |
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| author | Vanja Vlajkov Stefan Anđelić Ivana Pajčin Mila Grahovac Dragana Budakov Aleksandar Jokić Jovana Grahovac |
| author_facet | Vanja Vlajkov Stefan Anđelić Ivana Pajčin Mila Grahovac Dragana Budakov Aleksandar Jokić Jovana Grahovac |
| author_sort | Vanja Vlajkov |
| collection | DOAJ |
| description | One of the leading limiting factors for wider industrial production and commercialization of microbial biopesticides refers to the high costs of cultivation media. The selection of alternative sources of macronutrients crucial for the growth and metabolic activity of the producing microorganism is a necessary phase of the bioprocess development. Gaining a better understanding of the influence of the medium composition on the biotechnological production of biocontrol agents is enabled through bioprocess modelling and optimization. In the present study, after the selection of optimal carbon and nitrogen sources, two modelling approaches were applied to mathematically describe the behavior of the examined bioprocess—the production of biocontrol agents effective against aflatoxigenic <i>Aspergillus flavus</i> strains. The modelling was performed using four independent variables: cellulose, urea, ammonium sulfate and dipotassium phosphate, and the selected response was the inhibition-zone diameter. After the comparison of the results generated by the Response Surface Methodology (RSM) and the Artificial Neural Network (ANN) approach, the first model was chosen for the further optimization step due to the better fit of the experimental results. As the final investigation step, the optimal cultivation medium composition was defined (g/L): cellulose 5.0, ammonium sulfate 3.77, dipotassium phosphate 0.3, magnesium sulfate heptahydrate 0.3. |
| first_indexed | 2024-03-09T22:59:41Z |
| format | Article |
| id | doaj.art-0c316a6c9492429496145219fa0b7b7a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:59:41Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0c316a6c9492429496145219fa0b7b7a2023-11-23T18:04:03ZengMDPI AGMicroorganisms2076-26072022-06-01106116510.3390/microorganisms10061165Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and OptimizationVanja Vlajkov0Stefan Anđelić1Ivana Pajčin2Mila Grahovac3Dragana Budakov4Aleksandar Jokić5Jovana Grahovac6Faculty of Technology Novi Sad, University of Novi Sad, 21000 Novi Sad, SerbiaFaculty of Technical Sciences, University of Novi Sad, 21000 Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, 21000 Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, 21000 Novi Sad, SerbiaFaculty of Technology Novi Sad, University of Novi Sad, 21000 Novi Sad, SerbiaOne of the leading limiting factors for wider industrial production and commercialization of microbial biopesticides refers to the high costs of cultivation media. The selection of alternative sources of macronutrients crucial for the growth and metabolic activity of the producing microorganism is a necessary phase of the bioprocess development. Gaining a better understanding of the influence of the medium composition on the biotechnological production of biocontrol agents is enabled through bioprocess modelling and optimization. In the present study, after the selection of optimal carbon and nitrogen sources, two modelling approaches were applied to mathematically describe the behavior of the examined bioprocess—the production of biocontrol agents effective against aflatoxigenic <i>Aspergillus flavus</i> strains. The modelling was performed using four independent variables: cellulose, urea, ammonium sulfate and dipotassium phosphate, and the selected response was the inhibition-zone diameter. After the comparison of the results generated by the Response Surface Methodology (RSM) and the Artificial Neural Network (ANN) approach, the first model was chosen for the further optimization step due to the better fit of the experimental results. As the final investigation step, the optimal cultivation medium composition was defined (g/L): cellulose 5.0, ammonium sulfate 3.77, dipotassium phosphate 0.3, magnesium sulfate heptahydrate 0.3.https://www.mdpi.com/2076-2607/10/6/1165bioprocessANNRSMmodellingoptimizationbiocontrol |
| spellingShingle | Vanja Vlajkov Stefan Anđelić Ivana Pajčin Mila Grahovac Dragana Budakov Aleksandar Jokić Jovana Grahovac Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and Optimization Microorganisms bioprocess ANN RSM modelling optimization biocontrol |
| title | Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and Optimization |
| title_full | Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and Optimization |
| title_fullStr | Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and Optimization |
| title_full_unstemmed | Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and Optimization |
| title_short | Medium for the Production of <i>Bacillus</i>-Based Biocontrol Agent Effective against Aflatoxigenic <i>Aspergillus flavus</i>: Dual Approach for Modelling and Optimization |
| title_sort | medium for the production of i bacillus i based biocontrol agent effective against aflatoxigenic i aspergillus flavus i dual approach for modelling and optimization |
| topic | bioprocess ANN RSM modelling optimization biocontrol |
| url | https://www.mdpi.com/2076-2607/10/6/1165 |
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