Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic Rats
Background: <i>Ephedra foeminea</i> is known in Jordan as Alanda and traditionally. It is used to treat respiratory symptoms such as asthma and skin rashes as an infusion in boiling water. The purpose of this study was to determine the antidiabetic property of <i>Ephedra foeminea&l...
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MDPI AG
2022-06-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/11/2338 |
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| author | Maha N. Abu Hajleh Khaled M. Khleifat Moath Alqaraleh Esra’a Al-Hraishat Muhamad O. Al-limoun Haitham Qaralleh Emad A. S. Al-Dujaili |
| author_facet | Maha N. Abu Hajleh Khaled M. Khleifat Moath Alqaraleh Esra’a Al-Hraishat Muhamad O. Al-limoun Haitham Qaralleh Emad A. S. Al-Dujaili |
| author_sort | Maha N. Abu Hajleh |
| collection | DOAJ |
| description | Background: <i>Ephedra foeminea</i> is known in Jordan as Alanda and traditionally. It is used to treat respiratory symptoms such as asthma and skin rashes as an infusion in boiling water. The purpose of this study was to determine the antidiabetic property of <i>Ephedra foeminea</i> aqueous extract in streptozotocin-induced diabetic rats. Methods: The aqueous extract of <i>Ephedra foeminea</i> plant was used to determine the potential of its efficacy in the treatment of diabetes, and this extract was tested on diabetic rats as a model. The chemical composition of <i>Ephedra foeminea</i> aqueous extract was determined using liquid chromatography–mass spectrometry (LC-MS). Antioxidant activity was assessed using two classical assays (ABTS and DPPH). Results: The most abundant compounds in the <i>Ephedra foeminea</i> extract were limonene (6.3%), kaempferol (6.2%), stearic acid (5.9%), β-sitosterol (5.5%), thiamine (4.1%), riboflavin (3.1%), naringenin (2.8%), kaempferol-3-rhamnoside (2.3%), quercetin (2.2%), and ferulic acid (2.0%). The antioxidant activity of <i>Ephedra foeminea</i> aqueous extract was remarkable, as evidenced by radical scavenging capacities of 12.28 mg Trolox/g in ABTS and 72.8 mg GAE/g in DPPH. In comparison to control, induced diabetic rats treated with <i>Ephedra foeminea</i> extract showed significant improvement in blood glucose levels, lipid profile, liver, and kidney functions. Interleukin 1 and glutathione peroxidase levels in the spleen, pancreas, kidney, and liver of induced diabetic rats treated with <i>Ephedra foeminea</i> extract were significantly lower than in untreated diabetic rats. Conclusions: <i>Ephedra foeminea</i> aqueous extract appears to protect diabetic rats against oxidative stress and improve blood parameters. In addition, it has antioxidant properties that might be very beneficial medicinally. |
| first_indexed | 2024-03-10T00:59:17Z |
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| id | doaj.art-0c3315d8d8f44f41848195b41b1d3926 |
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| language | English |
| last_indexed | 2024-03-10T00:59:17Z |
| publishDate | 2022-06-01 |
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| series | Nutrients |
| spelling | doaj.art-0c3315d8d8f44f41848195b41b1d39262023-11-23T14:37:25ZengMDPI AGNutrients2072-66432022-06-011411233810.3390/nu14112338Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic RatsMaha N. Abu Hajleh0Khaled M. Khleifat1Moath Alqaraleh2Esra’a Al-Hraishat3Muhamad O. Al-limoun4Haitham Qaralleh5Emad A. S. Al-Dujaili6Department of Cosmetic Science, Pharmacological and Diagnostic Research Center, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman 19328, JordanFaculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman 19328, JordanPharmacological and Diagnostic Research Center, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, JordanDepartment of Biological Sciences, Mutah University, Mutah 61710, JordanDepartment of Biological Sciences, Mutah University, Mutah 61710, JordanDepartment of Medical Laboratory Sciences, Faculty of Science, Mutah University, Mutah 61710, JordanCentre for Cardiovascular Science, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh EH16 4JT, UKBackground: <i>Ephedra foeminea</i> is known in Jordan as Alanda and traditionally. It is used to treat respiratory symptoms such as asthma and skin rashes as an infusion in boiling water. The purpose of this study was to determine the antidiabetic property of <i>Ephedra foeminea</i> aqueous extract in streptozotocin-induced diabetic rats. Methods: The aqueous extract of <i>Ephedra foeminea</i> plant was used to determine the potential of its efficacy in the treatment of diabetes, and this extract was tested on diabetic rats as a model. The chemical composition of <i>Ephedra foeminea</i> aqueous extract was determined using liquid chromatography–mass spectrometry (LC-MS). Antioxidant activity was assessed using two classical assays (ABTS and DPPH). Results: The most abundant compounds in the <i>Ephedra foeminea</i> extract were limonene (6.3%), kaempferol (6.2%), stearic acid (5.9%), β-sitosterol (5.5%), thiamine (4.1%), riboflavin (3.1%), naringenin (2.8%), kaempferol-3-rhamnoside (2.3%), quercetin (2.2%), and ferulic acid (2.0%). The antioxidant activity of <i>Ephedra foeminea</i> aqueous extract was remarkable, as evidenced by radical scavenging capacities of 12.28 mg Trolox/g in ABTS and 72.8 mg GAE/g in DPPH. In comparison to control, induced diabetic rats treated with <i>Ephedra foeminea</i> extract showed significant improvement in blood glucose levels, lipid profile, liver, and kidney functions. Interleukin 1 and glutathione peroxidase levels in the spleen, pancreas, kidney, and liver of induced diabetic rats treated with <i>Ephedra foeminea</i> extract were significantly lower than in untreated diabetic rats. Conclusions: <i>Ephedra foeminea</i> aqueous extract appears to protect diabetic rats against oxidative stress and improve blood parameters. In addition, it has antioxidant properties that might be very beneficial medicinally.https://www.mdpi.com/2072-6643/14/11/2338<i>Ephedra foeminea</i>antioxidantantidiabeticglutathione peroxidaseinterleukin 1betaLC-MS |
| spellingShingle | Maha N. Abu Hajleh Khaled M. Khleifat Moath Alqaraleh Esra’a Al-Hraishat Muhamad O. Al-limoun Haitham Qaralleh Emad A. S. Al-Dujaili Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic Rats Nutrients <i>Ephedra foeminea</i> antioxidant antidiabetic glutathione peroxidase interleukin 1beta LC-MS |
| title | Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic Rats |
| title_full | Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic Rats |
| title_fullStr | Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic Rats |
| title_full_unstemmed | Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic Rats |
| title_short | Antioxidant and Antihyperglycemic Effects of <i>Ephedra foeminea</i> Aqueous Extract in Streptozotocin-Induced Diabetic Rats |
| title_sort | antioxidant and antihyperglycemic effects of i ephedra foeminea i aqueous extract in streptozotocin induced diabetic rats |
| topic | <i>Ephedra foeminea</i> antioxidant antidiabetic glutathione peroxidase interleukin 1beta LC-MS |
| url | https://www.mdpi.com/2072-6643/14/11/2338 |
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