Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer
Abstract Background Long non-coding RNAs (LncRNAs) are a class of newly identified transcripts recognized as critical governors of gene expression during human carcinogenesis, whereas their tumor-suppressive or tumor-promoting effects on gastric cancer (GC) are required for further investigation. In...
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BMC
2020-06-01
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| Series: | Cancer Cell International |
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| Online Access: | http://link.springer.com/article/10.1186/s12935-020-01369-7 |
| _version_ | 1818239911186137088 |
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| author | Xiongdong Zhong Xianchang Yu Xiaoyan Wen Lei Chen Ni Gu |
| author_facet | Xiongdong Zhong Xianchang Yu Xiaoyan Wen Lei Chen Ni Gu |
| author_sort | Xiongdong Zhong |
| collection | DOAJ |
| description | Abstract Background Long non-coding RNAs (LncRNAs) are a class of newly identified transcripts recognized as critical governors of gene expression during human carcinogenesis, whereas their tumor-suppressive or tumor-promoting effects on gastric cancer (GC) are required for further investigation. In the study, we identify the expression pattern of a novel lncRNA LINC00242 in GC and its possible permissive role in the development of GC. Methods The study included 68 pairs of GC and adjacent normal gastric tissue samples. The viability, migration, and invasion of cultured human GC cells HGC27 were evaluated by CCK-8 and Transwell chamber assays. In vitro tube formation of human brain microvascular endothelial cells (HBMVECs) in HGC27 cell coculture was detected. The regulatory network of LINC00242/miR-141/FOXC1 was verified using dual luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay. Subcutaneous xenografts of HGC27 cells were performed in nude mice. Results LINC00242 was highly expressed in GC tissues and cells and contributed to poor prognosis. LINC00242 knockdown inhibited HGC27 cell viability, migration and invasion, and tube formation of HBMVECs. LINC00242 interacted with miR-141 and positively regulated FOXC1, a target gene of miR-141. LINC00242 knockdown was partially lost in HGC27 cells upon miR-141 inhibition or FOXC1 overexpression. The tumor-promoting effect of LINC00242 on GC was demonstrated in nude mice. Conclusion Taken together, the present study demonstrates the oncogenic role of the LINC00242/miR-141/FOXC1 axis in GC, highlighting a theoretical basis for GC treatment. |
| first_indexed | 2024-12-12T13:05:04Z |
| format | Article |
| id | doaj.art-0c3489fd9af1462a9b6846ead847f10c |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-12T13:05:04Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-0c3489fd9af1462a9b6846ead847f10c2022-12-22T00:23:40ZengBMCCancer Cell International1475-28672020-06-0120111110.1186/s12935-020-01369-7Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancerXiongdong Zhong0Xianchang Yu1Xiaoyan Wen2Lei Chen3Ni Gu4Department of General Surgery, Zhuhai People’s Hospital (Zhuhai hospital affiliated with Jinan University)Department of General Surgery, Zhuhai People’s Hospital (Zhuhai hospital affiliated with Jinan University)Department of General Surgery, Zhuhai People’s Hospital (Zhuhai hospital affiliated with Jinan University)Department of General Surgery, Zhuhai People’s Hospital (Zhuhai hospital affiliated with Jinan University)Department of General Surgery, Zhuhai People’s Hospital (Zhuhai hospital affiliated with Jinan University)Abstract Background Long non-coding RNAs (LncRNAs) are a class of newly identified transcripts recognized as critical governors of gene expression during human carcinogenesis, whereas their tumor-suppressive or tumor-promoting effects on gastric cancer (GC) are required for further investigation. In the study, we identify the expression pattern of a novel lncRNA LINC00242 in GC and its possible permissive role in the development of GC. Methods The study included 68 pairs of GC and adjacent normal gastric tissue samples. The viability, migration, and invasion of cultured human GC cells HGC27 were evaluated by CCK-8 and Transwell chamber assays. In vitro tube formation of human brain microvascular endothelial cells (HBMVECs) in HGC27 cell coculture was detected. The regulatory network of LINC00242/miR-141/FOXC1 was verified using dual luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay. Subcutaneous xenografts of HGC27 cells were performed in nude mice. Results LINC00242 was highly expressed in GC tissues and cells and contributed to poor prognosis. LINC00242 knockdown inhibited HGC27 cell viability, migration and invasion, and tube formation of HBMVECs. LINC00242 interacted with miR-141 and positively regulated FOXC1, a target gene of miR-141. LINC00242 knockdown was partially lost in HGC27 cells upon miR-141 inhibition or FOXC1 overexpression. The tumor-promoting effect of LINC00242 on GC was demonstrated in nude mice. Conclusion Taken together, the present study demonstrates the oncogenic role of the LINC00242/miR-141/FOXC1 axis in GC, highlighting a theoretical basis for GC treatment.http://link.springer.com/article/10.1186/s12935-020-01369-7Gastric cancerLINC00242MicroRNA-141FOXC1 |
| spellingShingle | Xiongdong Zhong Xianchang Yu Xiaoyan Wen Lei Chen Ni Gu Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer Cancer Cell International Gastric cancer LINC00242 MicroRNA-141 FOXC1 |
| title | Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer |
| title_full | Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer |
| title_fullStr | Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer |
| title_full_unstemmed | Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer |
| title_short | Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer |
| title_sort | activation of the linc00242 mir 141 foxc1 axis underpins the development of gastric cancer |
| topic | Gastric cancer LINC00242 MicroRNA-141 FOXC1 |
| url | http://link.springer.com/article/10.1186/s12935-020-01369-7 |
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