Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone

In this experiment, corn stover was treated with optimal combined pretreatment conditions: 2% NaOH at 80 °C treated 2 h combined with initial pH 9 at the ozone concentration of 78 mg/mL treated 25 min. The effect of lignin removal rate on the enzymatic hydrolysis degree of cellulose during the treat...

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Main Authors: Shuo Fang, Wenhui Wang, Shisheng Tong, Chunyan Zhang, Ping Liu
Format: Article
Language:English
Published: MDPI AG 2018-06-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/23/6/1495
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author Shuo Fang
Wenhui Wang
Shisheng Tong
Chunyan Zhang
Ping Liu
author_facet Shuo Fang
Wenhui Wang
Shisheng Tong
Chunyan Zhang
Ping Liu
author_sort Shuo Fang
collection DOAJ
description In this experiment, corn stover was treated with optimal combined pretreatment conditions: 2% NaOH at 80 °C treated 2 h combined with initial pH 9 at the ozone concentration of 78 mg/mL treated 25 min. The effect of lignin removal rate on the enzymatic hydrolysis degree of cellulose during the treatment process was studied. At the same time, the lignin in the optimal pretreated corn stover was separated and extracted by enzymatic acidolysis, and its structure and connection were characterized. The results showed that the alkali combined with ozone pretreatment improved the enzymatic hydrolysis degree of the cellulose while exfoliating and degrading the macromolecular lignin into small molecules. The stable crosslink structure of the lignin-cellulose-hemicellulose was destroyed, and the lignocellulosic structure changed in favor of the enzymatic hydrolysis of the cellulose.
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spelling doaj.art-0c34f102665140838c2ff3b9ac0960c12022-12-21T18:23:39ZengMDPI AGMolecules1420-30492018-06-01236149510.3390/molecules23061495molecules23061495Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with OzoneShuo Fang0Wenhui Wang1Shisheng Tong2Chunyan Zhang3Ping Liu4College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaBio-Pharmaceutical College, Beijing City University, Beijing 100094, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaIn this experiment, corn stover was treated with optimal combined pretreatment conditions: 2% NaOH at 80 °C treated 2 h combined with initial pH 9 at the ozone concentration of 78 mg/mL treated 25 min. The effect of lignin removal rate on the enzymatic hydrolysis degree of cellulose during the treatment process was studied. At the same time, the lignin in the optimal pretreated corn stover was separated and extracted by enzymatic acidolysis, and its structure and connection were characterized. The results showed that the alkali combined with ozone pretreatment improved the enzymatic hydrolysis degree of the cellulose while exfoliating and degrading the macromolecular lignin into small molecules. The stable crosslink structure of the lignin-cellulose-hemicellulose was destroyed, and the lignocellulosic structure changed in favor of the enzymatic hydrolysis of the cellulose.http://www.mdpi.com/1420-3049/23/6/1495alkaline combined with ozonepretreatmentenzymatic hydrolysislignin
spellingShingle Shuo Fang
Wenhui Wang
Shisheng Tong
Chunyan Zhang
Ping Liu
Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone
Molecules
alkaline combined with ozone
pretreatment
enzymatic hydrolysis
lignin
title Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone
title_full Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone
title_fullStr Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone
title_full_unstemmed Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone
title_short Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone
title_sort evaluation of the effects of isolated lignin on cellulose enzymatic hydrolysis of corn stover pretreatment by naoh combined with ozone
topic alkaline combined with ozone
pretreatment
enzymatic hydrolysis
lignin
url http://www.mdpi.com/1420-3049/23/6/1495
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AT shishengtong evaluationoftheeffectsofisolatedligninoncelluloseenzymatichydrolysisofcornstoverpretreatmentbynaohcombinedwithozone
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