Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams
Abstract High levels of gums such as dextran, produced by Leuconostoc and Lactobacillus spp., have a severe impact on factory throughput and sugar quality. This study aimed to determine the phylogenetic relationships between gum‐producing Leuconostoc and Lactobacillus bacteria which were isolated fr...
| Main Authors: | , , , |
|---|---|
| פורמט: | Article |
| שפה: | English |
| יצא לאור: |
Wiley
2020-08-01
|
| סדרה: | MicrobiologyOpen |
| נושאים: | |
| גישה מקוונת: | https://doi.org/10.1002/mbo3.1065 |
| _version_ | 1828321616881254400 |
|---|---|
| author | Sanet Nel Stephen B. Davis Akihito Endo Leon M. T. Dicks |
| author_facet | Sanet Nel Stephen B. Davis Akihito Endo Leon M. T. Dicks |
| author_sort | Sanet Nel |
| collection | DOAJ |
| description | Abstract High levels of gums such as dextran, produced by Leuconostoc and Lactobacillus spp., have a severe impact on factory throughput and sugar quality. This study aimed to determine the phylogenetic relationships between gum‐producing Leuconostoc and Lactobacillus bacteria which were isolated from various locations in a sugarcane processing factory at times when low‐ and high‐dextran raw sugar, respectively, were produced. Phylogenetic analysis of 16S rRNA gene sequences grouped 81 isolates with the type strains of Leuconostoc mesenteroides (subspp. mesenteroides, dextranicum, and cremoris), Leuconostoc pseudomesenteroides, Leuconostoc lactis, and Leuconostoc citreum, respectively. Forty‐three isolates clustered with the type strain of Lactobacillus fermentum. The phylogenetic relatedness of the isolates was determined by sequencing and analysis of the housekeeping genes rpoA and dnaA for Leuconostoc spp. and the pheS and tuf genes for the Lactobacillus spp. The rpoA gene proved discriminatory for the phylogenetic resolution of all of the isolated Leuconostoc spp. and the dnaA housekeeping gene was shown to be effective for isolates clustering with the type strains of Leuc. mesenteroides and Leuc. citreum. None of the loci examined permitted differentiation at the subspecies level of Leuc. mesenteroides. Single‐locus analysis, as well as the concatenation of the pheS and tuf housekeeping gene sequences, yielded identical phylogenies for the Lactobacillus isolates corresponding to L. fermentum. |
| first_indexed | 2024-04-13T18:28:09Z |
| format | Article |
| id | doaj.art-0c3a35a98ff84e7ca9cf4373b51fc9c4 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-04-13T18:28:09Z |
| publishDate | 2020-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-0c3a35a98ff84e7ca9cf4373b51fc9c42022-12-22T02:35:10ZengWileyMicrobiologyOpen2045-88272020-08-0198n/an/a10.1002/mbo3.1065Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streamsSanet Nel0Stephen B. Davis1Akihito Endo2Leon M. T. Dicks3Sugar Milling Research Institute NPC c/o University of KwaZulu‐Natal Durban South AfricaSugar Milling Research Institute NPC c/o University of KwaZulu‐Natal Durban South AfricaDepartment of Food, Aroma and Cosmetic Chemistry Tokyo University of Agriculture Hokkaido JapanDepartment of Microbiology Stellenbosch University Stellenbosch South AfricaAbstract High levels of gums such as dextran, produced by Leuconostoc and Lactobacillus spp., have a severe impact on factory throughput and sugar quality. This study aimed to determine the phylogenetic relationships between gum‐producing Leuconostoc and Lactobacillus bacteria which were isolated from various locations in a sugarcane processing factory at times when low‐ and high‐dextran raw sugar, respectively, were produced. Phylogenetic analysis of 16S rRNA gene sequences grouped 81 isolates with the type strains of Leuconostoc mesenteroides (subspp. mesenteroides, dextranicum, and cremoris), Leuconostoc pseudomesenteroides, Leuconostoc lactis, and Leuconostoc citreum, respectively. Forty‐three isolates clustered with the type strain of Lactobacillus fermentum. The phylogenetic relatedness of the isolates was determined by sequencing and analysis of the housekeeping genes rpoA and dnaA for Leuconostoc spp. and the pheS and tuf genes for the Lactobacillus spp. The rpoA gene proved discriminatory for the phylogenetic resolution of all of the isolated Leuconostoc spp. and the dnaA housekeeping gene was shown to be effective for isolates clustering with the type strains of Leuc. mesenteroides and Leuc. citreum. None of the loci examined permitted differentiation at the subspecies level of Leuc. mesenteroides. Single‐locus analysis, as well as the concatenation of the pheS and tuf housekeeping gene sequences, yielded identical phylogenies for the Lactobacillus isolates corresponding to L. fermentum.https://doi.org/10.1002/mbo3.1065dextranLactobacillusLeuconostocphylogenetic analysissugarcane |
| spellingShingle | Sanet Nel Stephen B. Davis Akihito Endo Leon M. T. Dicks Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams MicrobiologyOpen dextran Lactobacillus Leuconostoc phylogenetic analysis sugarcane |
| title | Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams |
| title_full | Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams |
| title_fullStr | Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams |
| title_full_unstemmed | Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams |
| title_short | Phylogenetic analysis of Leuconostoc and Lactobacillus species isolated from sugarcane processing streams |
| title_sort | phylogenetic analysis of leuconostoc and lactobacillus species isolated from sugarcane processing streams |
| topic | dextran Lactobacillus Leuconostoc phylogenetic analysis sugarcane |
| url | https://doi.org/10.1002/mbo3.1065 |
| work_keys_str_mv | AT sanetnel phylogeneticanalysisofleuconostocandlactobacillusspeciesisolatedfromsugarcaneprocessingstreams AT stephenbdavis phylogeneticanalysisofleuconostocandlactobacillusspeciesisolatedfromsugarcaneprocessingstreams AT akihitoendo phylogeneticanalysisofleuconostocandlactobacillusspeciesisolatedfromsugarcaneprocessingstreams AT leonmtdicks phylogeneticanalysisofleuconostocandlactobacillusspeciesisolatedfromsugarcaneprocessingstreams |