On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation
Here the authors observe transcription dynamics in real-time throughout erythropoiesis by combining PP7 tagging of α-globin RNA transcripts and “on-microscope” cell staging. They show how the pattern of transcriptional bursting changes during differentiation, with variability in individual cells sig...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-33977-4 |
| _version_ | 1798044282864533504 |
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| author | D. M. Jeziorska E. A. J. Tunnacliffe J. M. Brown H. Ayyub J. Sloane-Stanley J. A. Sharpe B. C. Lagerholm C. Babbs A. J. H. Smith V. J. Buckle D. R. Higgs |
| author_facet | D. M. Jeziorska E. A. J. Tunnacliffe J. M. Brown H. Ayyub J. Sloane-Stanley J. A. Sharpe B. C. Lagerholm C. Babbs A. J. H. Smith V. J. Buckle D. R. Higgs |
| author_sort | D. M. Jeziorska |
| collection | DOAJ |
| description | Here the authors observe transcription dynamics in real-time throughout erythropoiesis by combining PP7 tagging of α-globin RNA transcripts and “on-microscope” cell staging. They show how the pattern of transcriptional bursting changes during differentiation, with variability in individual cells significantly reduced at the peak period of gene expression. |
| first_indexed | 2024-04-11T23:01:17Z |
| format | Article |
| id | doaj.art-0c3b40eaebc94c59a4d6b6a4888b986e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T23:01:17Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-0c3b40eaebc94c59a4d6b6a4888b986e2022-12-22T03:58:07ZengNature PortfolioNature Communications2041-17232022-11-0113111210.1038/s41467-022-33977-4On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiationD. M. Jeziorska0E. A. J. Tunnacliffe1J. M. Brown2H. Ayyub3J. Sloane-Stanley4J. A. Sharpe5B. C. Lagerholm6C. Babbs7A. J. H. Smith8V. J. Buckle9D. R. Higgs10MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalWolfson Imaging Centre, MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalMRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe HospitalHere the authors observe transcription dynamics in real-time throughout erythropoiesis by combining PP7 tagging of α-globin RNA transcripts and “on-microscope” cell staging. They show how the pattern of transcriptional bursting changes during differentiation, with variability in individual cells significantly reduced at the peak period of gene expression.https://doi.org/10.1038/s41467-022-33977-4 |
| spellingShingle | D. M. Jeziorska E. A. J. Tunnacliffe J. M. Brown H. Ayyub J. Sloane-Stanley J. A. Sharpe B. C. Lagerholm C. Babbs A. J. H. Smith V. J. Buckle D. R. Higgs On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation Nature Communications |
| title | On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation |
| title_full | On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation |
| title_fullStr | On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation |
| title_full_unstemmed | On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation |
| title_short | On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation |
| title_sort | on microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation |
| url | https://doi.org/10.1038/s41467-022-33977-4 |
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