Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathways
<p>Abstract</p> <p>Background</p> <p>Root-knot nematodes are sedentary endoparasites that can infect more than 3000 plant species. Root-knot nematodes cause an estimated $100 billion annual loss worldwide. For successful establishment of the root-knot nematode in its ho...
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| Format: | Article |
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BMC
2011-05-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/12/220 |
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| author | Gamal El-Din Abd El Kader Y Hussein Ebtissam HA Alkharouf Nadim W Hosseini Parsa Ibrahim Heba MM Aly Mohammed AM Matthews Benjamin F |
| author_facet | Gamal El-Din Abd El Kader Y Hussein Ebtissam HA Alkharouf Nadim W Hosseini Parsa Ibrahim Heba MM Aly Mohammed AM Matthews Benjamin F |
| author_sort | Gamal El-Din Abd El Kader Y |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Root-knot nematodes are sedentary endoparasites that can infect more than 3000 plant species. Root-knot nematodes cause an estimated $100 billion annual loss worldwide. For successful establishment of the root-knot nematode in its host plant, it causes dramatic morphological and physiological changes in plant cells. The expression of some plant genes is altered by the nematode as it establishes its feeding site.</p> <p>Results</p> <p>We examined the expression of soybean (<it>Glycine max</it>) genes in galls formed in roots by the root-knot nematode, <it>Meloidogyne incognita</it>, 12 days and 10 weeks after infection to understand the effects of infection of roots by <it>M. incognita</it>. Gene expression was monitored using the Affymetrix Soybean GeneChip containing 37,500 <it>G. max </it>probe sets. Gene expression patterns were integrated with biochemical pathways from the Kyoto Encyclopedia of Genes and Genomes using PAICE software. Genes encoding enzymes involved in carbohydrate and cell wall metabolism, cell cycle control and plant defense were altered.</p> <p>Conclusions</p> <p>A number of different soybean genes were identified that were differentially expressed which provided insights into the interaction between <it>M. incognita </it>and soybean and into the formation and maintenance of giant cells. Some of these genes may be candidates for broadening plants resistance to root-knot nematode through over-expression or silencing and require further examination.</p> |
| first_indexed | 2024-12-17T00:30:46Z |
| format | Article |
| id | doaj.art-0c4059a2235741b79c2fe50ef2ff820b |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T00:30:46Z |
| publishDate | 2011-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-0c4059a2235741b79c2fe50ef2ff820b2022-12-21T22:10:19ZengBMCBMC Genomics1471-21642011-05-0112122010.1186/1471-2164-12-220Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathwaysGamal El-Din Abd El Kader YHussein Ebtissam HAAlkharouf Nadim WHosseini ParsaIbrahim Heba MMAly Mohammed AMMatthews Benjamin F<p>Abstract</p> <p>Background</p> <p>Root-knot nematodes are sedentary endoparasites that can infect more than 3000 plant species. Root-knot nematodes cause an estimated $100 billion annual loss worldwide. For successful establishment of the root-knot nematode in its host plant, it causes dramatic morphological and physiological changes in plant cells. The expression of some plant genes is altered by the nematode as it establishes its feeding site.</p> <p>Results</p> <p>We examined the expression of soybean (<it>Glycine max</it>) genes in galls formed in roots by the root-knot nematode, <it>Meloidogyne incognita</it>, 12 days and 10 weeks after infection to understand the effects of infection of roots by <it>M. incognita</it>. Gene expression was monitored using the Affymetrix Soybean GeneChip containing 37,500 <it>G. max </it>probe sets. Gene expression patterns were integrated with biochemical pathways from the Kyoto Encyclopedia of Genes and Genomes using PAICE software. Genes encoding enzymes involved in carbohydrate and cell wall metabolism, cell cycle control and plant defense were altered.</p> <p>Conclusions</p> <p>A number of different soybean genes were identified that were differentially expressed which provided insights into the interaction between <it>M. incognita </it>and soybean and into the formation and maintenance of giant cells. Some of these genes may be candidates for broadening plants resistance to root-knot nematode through over-expression or silencing and require further examination.</p>http://www.biomedcentral.com/1471-2164/12/220 |
| spellingShingle | Gamal El-Din Abd El Kader Y Hussein Ebtissam HA Alkharouf Nadim W Hosseini Parsa Ibrahim Heba MM Aly Mohammed AM Matthews Benjamin F Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathways BMC Genomics |
| title | Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathways |
| title_full | Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathways |
| title_fullStr | Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathways |
| title_full_unstemmed | Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathways |
| title_short | Analysis of Gene expression in soybean (<it>Glycine max</it>) roots in response to the root knot nematode <it>Meloidogyne incognita </it>using microarrays and KEGG pathways |
| title_sort | analysis of gene expression in soybean it glycine max it roots in response to the root knot nematode it meloidogyne incognita it using microarrays and kegg pathways |
| url | http://www.biomedcentral.com/1471-2164/12/220 |
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