Deproteinization of Chitin Extracted with the Help of Ionic Liquids
The isolation of chitin utilizing ionic liquid 1-ethyl-3-methylimidazolium acetate has been determined to result in polymer contaminated with proteins. For the first time, the proteins in chitin extracted with ionic liquid have been quantified; the protein content was found to vary from 1.3 to 1.9%...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/3983 |
| _version_ | 1797442681749635072 |
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| author | Douglas R. Lyon Bryan R. Smith Noureddine Abidi Julia L. Shamshina |
| author_facet | Douglas R. Lyon Bryan R. Smith Noureddine Abidi Julia L. Shamshina |
| author_sort | Douglas R. Lyon |
| collection | DOAJ |
| description | The isolation of chitin utilizing ionic liquid 1-ethyl-3-methylimidazolium acetate has been determined to result in polymer contaminated with proteins. For the first time, the proteins in chitin extracted with ionic liquid have been quantified; the protein content was found to vary from 1.3 to 1.9% of the total weight. These proteins were identified and include allergenic proteins such as tropomyosin. In order to avoid ‘traditional’ hydroxide-based deproteinization of chitin, which could reduce the molecular weight of the final product, alternative deproteinization strategies were attempted. Testing of the previously reported deproteinization method using aqueous K<sub>3</sub>PO<sub>4</sub> resulted in protein reduction by factors varying from 2 to 10, but resulted in significant phosphate salt contamination of the final product. Contrarily, the incorporation of GRAS (Generally Recognized as Safe) compound Polysorbate 80 into the polymer washing step provided the polymer of comparable purity with no contaminants. This study presents new options for the deproteinization of chitin that can replace traditional approaches with methods that are environmentally friendly and can produce high purity polymer. |
| first_indexed | 2024-03-09T12:45:30Z |
| format | Article |
| id | doaj.art-0c45fb23264645a49195ca1402efcb49 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T12:45:30Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0c45fb23264645a49195ca1402efcb492023-11-30T22:12:41ZengMDPI AGMolecules1420-30492022-06-012713398310.3390/molecules27133983Deproteinization of Chitin Extracted with the Help of Ionic LiquidsDouglas R. Lyon0Bryan R. Smith1Noureddine Abidi2Julia L. Shamshina3Lummus Technology, Houston, TX 77086, USAInBio, Charlottesville, VA 22903, USAFiber and Biopolymer Research Institute, Texas Tech University, Lubbock, TX 79409, USAFiber and Biopolymer Research Institute, Texas Tech University, Lubbock, TX 79409, USAThe isolation of chitin utilizing ionic liquid 1-ethyl-3-methylimidazolium acetate has been determined to result in polymer contaminated with proteins. For the first time, the proteins in chitin extracted with ionic liquid have been quantified; the protein content was found to vary from 1.3 to 1.9% of the total weight. These proteins were identified and include allergenic proteins such as tropomyosin. In order to avoid ‘traditional’ hydroxide-based deproteinization of chitin, which could reduce the molecular weight of the final product, alternative deproteinization strategies were attempted. Testing of the previously reported deproteinization method using aqueous K<sub>3</sub>PO<sub>4</sub> resulted in protein reduction by factors varying from 2 to 10, but resulted in significant phosphate salt contamination of the final product. Contrarily, the incorporation of GRAS (Generally Recognized as Safe) compound Polysorbate 80 into the polymer washing step provided the polymer of comparable purity with no contaminants. This study presents new options for the deproteinization of chitin that can replace traditional approaches with methods that are environmentally friendly and can produce high purity polymer.https://www.mdpi.com/1420-3049/27/13/3983ionic liquidchitindeproteinizationPolysorbate 80 (Tween<sup>®</sup> 80) |
| spellingShingle | Douglas R. Lyon Bryan R. Smith Noureddine Abidi Julia L. Shamshina Deproteinization of Chitin Extracted with the Help of Ionic Liquids Molecules ionic liquid chitin deproteinization Polysorbate 80 (Tween<sup>®</sup> 80) |
| title | Deproteinization of Chitin Extracted with the Help of Ionic Liquids |
| title_full | Deproteinization of Chitin Extracted with the Help of Ionic Liquids |
| title_fullStr | Deproteinization of Chitin Extracted with the Help of Ionic Liquids |
| title_full_unstemmed | Deproteinization of Chitin Extracted with the Help of Ionic Liquids |
| title_short | Deproteinization of Chitin Extracted with the Help of Ionic Liquids |
| title_sort | deproteinization of chitin extracted with the help of ionic liquids |
| topic | ionic liquid chitin deproteinization Polysorbate 80 (Tween<sup>®</sup> 80) |
| url | https://www.mdpi.com/1420-3049/27/13/3983 |
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