A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High Temperatures
High temperatures delay tuberization and decrease potato (<i>Solanum tuberosum</i> L.) yields. However, the molecular mechanisms and regulatory networks underlying tuberization under high temperatures remain largely unknown. Here, we performed the mRNA and miRNA sequencing of leaves and...
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MDPI AG
2024-03-01
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| Online Access: | https://www.mdpi.com/2223-7747/13/7/998 |
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| author | Ming He Ju Liu Jie Tan Yinqiao Jian Jiangang Liu Yanfeng Duan Guangcun Li Liping Jin Jianfei Xu |
| author_facet | Ming He Ju Liu Jie Tan Yinqiao Jian Jiangang Liu Yanfeng Duan Guangcun Li Liping Jin Jianfei Xu |
| author_sort | Ming He |
| collection | DOAJ |
| description | High temperatures delay tuberization and decrease potato (<i>Solanum tuberosum</i> L.) yields. However, the molecular mechanisms and regulatory networks underlying tuberization under high temperatures remain largely unknown. Here, we performed the mRNA and miRNA sequencing of leaves and stems to identify genes and regulatory networks involved in tuberization under high temperatures. A total of 2804 and 5001 differentially expressed genes (DEGs) under high-temperature stress were identified in leaves and stems, respectively. These genes were significantly enriched in gene ontology terms regarding meristem development, the sucrose biosynthetic process, and response to heat. Meanwhile, 101 and 75 differentially expressed miRNAs (DEmiRNAs) were identified in leaves and stems, respectively. We constructed an interaction network between DEmiRNAs and DEGs, identifying 118 and 150 DEmiRNA–DEG pairs in leaves and stems, respectively. We found three miRNA–mRNA candidate modules involved in tuberization under high temperatures, including <i>stu-miR8030-5p</i>/<i>StCPY714</i>, <i>stu-miR7981f-p5</i>/<i>StAGL8a</i>, and <i>stu-miR10532A</i>/<i>StAGL8b</i>. Our study constructed an interaction network between miRNAs and target genes and proposes candidate miRNA–gene modules that regulate tuber formation under high temperatures. Our study provides new insights for revealing the regulatory mechanism of the high-temperature inhibition of tuberization and also provides gene resources for improving the heat tolerance in potatoes. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-24T10:36:22Z |
| publishDate | 2024-03-01 |
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| series | Plants |
| spelling | doaj.art-0c460326cf754040a6465f0934b0e1e52024-04-12T13:24:49ZengMDPI AGPlants2223-77472024-03-0113799810.3390/plants13070998A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High TemperaturesMing He0Ju Liu1Jie Tan2Yinqiao Jian3Jiangang Liu4Yanfeng Duan5Guangcun Li6Liping Jin7Jianfei Xu8Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaHigh temperatures delay tuberization and decrease potato (<i>Solanum tuberosum</i> L.) yields. However, the molecular mechanisms and regulatory networks underlying tuberization under high temperatures remain largely unknown. Here, we performed the mRNA and miRNA sequencing of leaves and stems to identify genes and regulatory networks involved in tuberization under high temperatures. A total of 2804 and 5001 differentially expressed genes (DEGs) under high-temperature stress were identified in leaves and stems, respectively. These genes were significantly enriched in gene ontology terms regarding meristem development, the sucrose biosynthetic process, and response to heat. Meanwhile, 101 and 75 differentially expressed miRNAs (DEmiRNAs) were identified in leaves and stems, respectively. We constructed an interaction network between DEmiRNAs and DEGs, identifying 118 and 150 DEmiRNA–DEG pairs in leaves and stems, respectively. We found three miRNA–mRNA candidate modules involved in tuberization under high temperatures, including <i>stu-miR8030-5p</i>/<i>StCPY714</i>, <i>stu-miR7981f-p5</i>/<i>StAGL8a</i>, and <i>stu-miR10532A</i>/<i>StAGL8b</i>. Our study constructed an interaction network between miRNAs and target genes and proposes candidate miRNA–gene modules that regulate tuber formation under high temperatures. Our study provides new insights for revealing the regulatory mechanism of the high-temperature inhibition of tuberization and also provides gene resources for improving the heat tolerance in potatoes.https://www.mdpi.com/2223-7747/13/7/998potatotuberizationhigh temperatureRNA sequencingmiRNA sequencing |
| spellingShingle | Ming He Ju Liu Jie Tan Yinqiao Jian Jiangang Liu Yanfeng Duan Guangcun Li Liping Jin Jianfei Xu A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High Temperatures Plants potato tuberization high temperature RNA sequencing miRNA sequencing |
| title | A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High Temperatures |
| title_full | A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High Temperatures |
| title_fullStr | A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High Temperatures |
| title_full_unstemmed | A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High Temperatures |
| title_short | A Comprehensive Interaction Network Constructed Using miRNAs and mRNAs Provides New Insights into Potato Tuberization under High Temperatures |
| title_sort | comprehensive interaction network constructed using mirnas and mrnas provides new insights into potato tuberization under high temperatures |
| topic | potato tuberization high temperature RNA sequencing miRNA sequencing |
| url | https://www.mdpi.com/2223-7747/13/7/998 |
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