The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and Bioinformatics
Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial lung disease. Currently, therapeutic options are limited for this fatal disease. Curcumin, with its pleiotropic effects, has been studied for its potential therapeutic utilities in various diseases, including pulmonary fib...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/22/5458 |
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| author | Wei-An Chang Chia-Min Chen Chau-Chyun Sheu Ssu-Hui Liao Ya-Ling Hsu Ming-Ju Tsai Po-Lin Kuo |
| author_facet | Wei-An Chang Chia-Min Chen Chau-Chyun Sheu Ssu-Hui Liao Ya-Ling Hsu Ming-Ju Tsai Po-Lin Kuo |
| author_sort | Wei-An Chang |
| collection | DOAJ |
| description | Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial lung disease. Currently, therapeutic options are limited for this fatal disease. Curcumin, with its pleiotropic effects, has been studied for its potential therapeutic utilities in various diseases, including pulmonary fibrosis. However, the detailed mechanisms have not been studied comprehensively. We conducted a next-generation sequencing and bioinformatics study to investigate changes in the profiles of mRNA and microRNA after curcumin treatment in IPF fibroblasts. We identified 23 downregulated and 8 upregulated protein-coding genes in curcumin-treated IPF fibroblasts. Using STRING and IPA, we identified that suppression of cell cycle progression was the main cellular function associated with these differentially expressed genes. We also identified 13 downregulated and 57 upregulated microRNAs in curcumin-treated IPF fibroblasts. Further analysis identified a potential microRNA-mediated gene expression alteration in curcumin-treated IPF fibroblasts, namely, downregulated hsa-miR-6724-5p and upregulated <i>KLF10</i>. Therefore, curcumin might decrease the level of hsa-miR-6724-5p, leading to increased <i>KLF10</i> expression, resulting in cell cycle arrest in curcumin-treated IPF fibroblasts. In conclusion, our findings might support the potential role of curcumin in the treatment of IPF, but further in-depth study is warranted to confirm our findings. |
| first_indexed | 2024-03-10T14:40:34Z |
| format | Article |
| id | doaj.art-0c47ec66a38c4c1b814a197d08d1bfe7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:40:34Z |
| publishDate | 2020-11-01 |
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| series | Molecules |
| spelling | doaj.art-0c47ec66a38c4c1b814a197d08d1bfe72023-11-20T21:48:06ZengMDPI AGMolecules1420-30492020-11-012522545810.3390/molecules25225458The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and BioinformaticsWei-An Chang0Chia-Min Chen1Chau-Chyun Sheu2Ssu-Hui Liao3Ya-Ling Hsu4Ming-Ju Tsai5Po-Lin Kuo6Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanGraduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanIdiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial lung disease. Currently, therapeutic options are limited for this fatal disease. Curcumin, with its pleiotropic effects, has been studied for its potential therapeutic utilities in various diseases, including pulmonary fibrosis. However, the detailed mechanisms have not been studied comprehensively. We conducted a next-generation sequencing and bioinformatics study to investigate changes in the profiles of mRNA and microRNA after curcumin treatment in IPF fibroblasts. We identified 23 downregulated and 8 upregulated protein-coding genes in curcumin-treated IPF fibroblasts. Using STRING and IPA, we identified that suppression of cell cycle progression was the main cellular function associated with these differentially expressed genes. We also identified 13 downregulated and 57 upregulated microRNAs in curcumin-treated IPF fibroblasts. Further analysis identified a potential microRNA-mediated gene expression alteration in curcumin-treated IPF fibroblasts, namely, downregulated hsa-miR-6724-5p and upregulated <i>KLF10</i>. Therefore, curcumin might decrease the level of hsa-miR-6724-5p, leading to increased <i>KLF10</i> expression, resulting in cell cycle arrest in curcumin-treated IPF fibroblasts. In conclusion, our findings might support the potential role of curcumin in the treatment of IPF, but further in-depth study is warranted to confirm our findings.https://www.mdpi.com/1420-3049/25/22/5458bioinformaticscurcuminfibroblastsidiopathic pulmonary fibrosismicroRNAnext-generation sequencing |
| spellingShingle | Wei-An Chang Chia-Min Chen Chau-Chyun Sheu Ssu-Hui Liao Ya-Ling Hsu Ming-Ju Tsai Po-Lin Kuo The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and Bioinformatics Molecules bioinformatics curcumin fibroblasts idiopathic pulmonary fibrosis microRNA next-generation sequencing |
| title | The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and Bioinformatics |
| title_full | The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and Bioinformatics |
| title_fullStr | The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and Bioinformatics |
| title_full_unstemmed | The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and Bioinformatics |
| title_short | The Potential Effects of Curcumin on Pulmonary Fibroblasts of Idiopathic Pulmonary Fibrosis (IPF)—Approaching with Next-Generation Sequencing and Bioinformatics |
| title_sort | potential effects of curcumin on pulmonary fibroblasts of idiopathic pulmonary fibrosis ipf approaching with next generation sequencing and bioinformatics |
| topic | bioinformatics curcumin fibroblasts idiopathic pulmonary fibrosis microRNA next-generation sequencing |
| url | https://www.mdpi.com/1420-3049/25/22/5458 |
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