Development of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animal
Abstract Spiral ligament fibrocytes generate potassium gradients, which hair cells require to convert mechanical sound waves into electrical palsy. Together with the stria vascularis, they regulate endolymph electrolyte homeostasis. Developing spiral ligament fibrocytes and generating endocochlear p...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39003-x |
| _version_ | 1797774195997802496 |
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| author | Makoto Hosoya Kaho Iwabu Tsubasa Kitama Takanori Nishiyama Naoki Oishi Hideyuki Okano Hiroyuki Ozawa |
| author_facet | Makoto Hosoya Kaho Iwabu Tsubasa Kitama Takanori Nishiyama Naoki Oishi Hideyuki Okano Hiroyuki Ozawa |
| author_sort | Makoto Hosoya |
| collection | DOAJ |
| description | Abstract Spiral ligament fibrocytes generate potassium gradients, which hair cells require to convert mechanical sound waves into electrical palsy. Together with the stria vascularis, they regulate endolymph electrolyte homeostasis. Developing spiral ligament fibrocytes and generating endocochlear potential with an appropriate endolymph ion composition are essential for hearing. Understanding spiral ligament fibrocyte development is useful for studying age-related and genetic hearing loss, as well as for regenerative therapy and cochlear immunology. Despite interspecies differences, most studies of cochlear development have been conducted in rodent models due to the difficulty of using human fetal samples. This study investigated the cochlear development of spiral ligament fibrocytes in a small New World monkey species, the common marmoset (Callithrix jacchus). We examined the developmental expression of specific genes in spiral ligament fibrocytes, including those essential for the generation of endolymphatic potential. Our results showed that this animal model of spiral ligament fibrocyte development is similar to that of humans and is a suitable alternative for the analysis of human cochlear development. The time course established in this study will be useful for studying the primate-specific developmental biology of the inner ear, which may lead to novel treatment strategies for human hearing loss. |
| first_indexed | 2024-03-12T22:17:29Z |
| format | Article |
| id | doaj.art-0c48e04f0408447483cdb404b67811f1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:17:29Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0c48e04f0408447483cdb404b67811f12023-07-23T11:13:43ZengNature PortfolioScientific Reports2045-23222023-07-0113111210.1038/s41598-023-39003-xDevelopment of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animalMakoto Hosoya0Kaho Iwabu1Tsubasa Kitama2Takanori Nishiyama3Naoki Oishi4Hideyuki Okano5Hiroyuki Ozawa6Department of Otolaryngology, Head and Neck Surgery, Keio University School of MedicineDepartment of Otolaryngology, Head and Neck Surgery, Keio University School of MedicineDepartment of Otolaryngology, Head and Neck Surgery, Keio University School of MedicineDepartment of Otolaryngology, Head and Neck Surgery, Keio University School of MedicineDepartment of Otolaryngology, Head and Neck Surgery, Keio University School of MedicineDepartment of Physiology, Keio University School of MedicineDepartment of Otolaryngology, Head and Neck Surgery, Keio University School of MedicineAbstract Spiral ligament fibrocytes generate potassium gradients, which hair cells require to convert mechanical sound waves into electrical palsy. Together with the stria vascularis, they regulate endolymph electrolyte homeostasis. Developing spiral ligament fibrocytes and generating endocochlear potential with an appropriate endolymph ion composition are essential for hearing. Understanding spiral ligament fibrocyte development is useful for studying age-related and genetic hearing loss, as well as for regenerative therapy and cochlear immunology. Despite interspecies differences, most studies of cochlear development have been conducted in rodent models due to the difficulty of using human fetal samples. This study investigated the cochlear development of spiral ligament fibrocytes in a small New World monkey species, the common marmoset (Callithrix jacchus). We examined the developmental expression of specific genes in spiral ligament fibrocytes, including those essential for the generation of endolymphatic potential. Our results showed that this animal model of spiral ligament fibrocyte development is similar to that of humans and is a suitable alternative for the analysis of human cochlear development. The time course established in this study will be useful for studying the primate-specific developmental biology of the inner ear, which may lead to novel treatment strategies for human hearing loss.https://doi.org/10.1038/s41598-023-39003-x |
| spellingShingle | Makoto Hosoya Kaho Iwabu Tsubasa Kitama Takanori Nishiyama Naoki Oishi Hideyuki Okano Hiroyuki Ozawa Development of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animal Scientific Reports |
| title | Development of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animal |
| title_full | Development of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animal |
| title_fullStr | Development of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animal |
| title_full_unstemmed | Development of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animal |
| title_short | Development of cochlear spiral ligament fibrocytes of the common marmoset, a nonhuman model animal |
| title_sort | development of cochlear spiral ligament fibrocytes of the common marmoset a nonhuman model animal |
| url | https://doi.org/10.1038/s41598-023-39003-x |
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