Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells
<p>Abstract</p> <p>Background</p> <p>Electrospun nanofibers have been widely used as substrata for mammalian cell culture owing to their structural similarity to natural extracellular matrices. Structurally consistent electrospun nanofibers can be produced with syntheti...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-06-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://www.jnanobiotechnology.com/content/10/1/23 |
| _version_ | 1798006431585140736 |
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| author | Jung Dongju Minami Itsunari Patel Sahishnu Lee Jonghwan Jiang Bin Yuan Qinghua Li Liu Kobayashi Sachiko Chen Yong Lee Ki-Bum Nakatsuji Norio |
| author_facet | Jung Dongju Minami Itsunari Patel Sahishnu Lee Jonghwan Jiang Bin Yuan Qinghua Li Liu Kobayashi Sachiko Chen Yong Lee Ki-Bum Nakatsuji Norio |
| author_sort | Jung Dongju |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Electrospun nanofibers have been widely used as substrata for mammalian cell culture owing to their structural similarity to natural extracellular matrices. Structurally consistent electrospun nanofibers can be produced with synthetic polymers but require chemical modification to graft cell-adhesive molecules to make the nanofibers functional. Development of a facile method of grafting functional molecules on the nanofibers will contribute to the production of diverse cell type-specific nanofiber substrata.</p> <p>Results</p> <p>Small molecules, peptides, and functionalized gold nanoparticles were successfully incorporated with polymethylglutarimide (PMGI) nanofibers through electrospinning. The PMGI nanofibers functionalized by the grafted AuNPs, which were labeled with cell-adhesive peptides, enhanced HeLa cell attachment and potentiated cardiomyocyte differentiation of human pluripotent stem cells.</p> <p>Conclusions</p> <p>PMGI nanofibers can be functionalized simply by co-electrospinning with the grafting materials. In addition, grafting functionalized AuNPs enable high-density localization of the cell-adhesive peptides on the nanofiber. The results of the present study suggest that more cell type-specific synthetic substrata can be fabricated with molecule-doped nanofibers, in which diverse functional molecules are grafted alone or in combination with other molecules at different concentrations.</p> |
| first_indexed | 2024-04-11T12:55:44Z |
| format | Article |
| id | doaj.art-0c4ad3a4895e4f3bbdb836ea7e37473d |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T12:55:44Z |
| publishDate | 2012-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-0c4ad3a4895e4f3bbdb836ea7e37473d2022-12-22T04:23:05ZengBMCJournal of Nanobiotechnology1477-31552012-06-011012310.1186/1477-3155-10-23Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cellsJung DongjuMinami ItsunariPatel SahishnuLee JonghwanJiang BinYuan QinghuaLi LiuKobayashi SachikoChen YongLee Ki-BumNakatsuji Norio<p>Abstract</p> <p>Background</p> <p>Electrospun nanofibers have been widely used as substrata for mammalian cell culture owing to their structural similarity to natural extracellular matrices. Structurally consistent electrospun nanofibers can be produced with synthetic polymers but require chemical modification to graft cell-adhesive molecules to make the nanofibers functional. Development of a facile method of grafting functional molecules on the nanofibers will contribute to the production of diverse cell type-specific nanofiber substrata.</p> <p>Results</p> <p>Small molecules, peptides, and functionalized gold nanoparticles were successfully incorporated with polymethylglutarimide (PMGI) nanofibers through electrospinning. The PMGI nanofibers functionalized by the grafted AuNPs, which were labeled with cell-adhesive peptides, enhanced HeLa cell attachment and potentiated cardiomyocyte differentiation of human pluripotent stem cells.</p> <p>Conclusions</p> <p>PMGI nanofibers can be functionalized simply by co-electrospinning with the grafting materials. In addition, grafting functionalized AuNPs enable high-density localization of the cell-adhesive peptides on the nanofiber. The results of the present study suggest that more cell type-specific synthetic substrata can be fabricated with molecule-doped nanofibers, in which diverse functional molecules are grafted alone or in combination with other molecules at different concentrations.</p>http://www.jnanobiotechnology.com/content/10/1/23Molecule-doped nanofiberSynthetic nanofiber substrataFunctionalized gold nanoparticles |
| spellingShingle | Jung Dongju Minami Itsunari Patel Sahishnu Lee Jonghwan Jiang Bin Yuan Qinghua Li Liu Kobayashi Sachiko Chen Yong Lee Ki-Bum Nakatsuji Norio Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells Journal of Nanobiotechnology Molecule-doped nanofiber Synthetic nanofiber substrata Functionalized gold nanoparticles |
| title | Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells |
| title_full | Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells |
| title_fullStr | Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells |
| title_full_unstemmed | Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells |
| title_short | Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells |
| title_sort | incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells |
| topic | Molecule-doped nanofiber Synthetic nanofiber substrata Functionalized gold nanoparticles |
| url | http://www.jnanobiotechnology.com/content/10/1/23 |
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