Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface
Background: The purpose of this study was to investigate the anti-oral microbial activity and anti-inflammatory effects of rosmarinic acid (RA) in lipopolysaccharide (LPS)-stimulated MC3T3-E1 osteoblastic cells on a titanium (Ti) surface during osseointegration, and to confirm the possibility of usi...
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| Format: | Article |
| Language: | English |
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The Korean Society of Dental Hygiene Science
2020-12-01
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| Series: | 치위생과학회지 |
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| _version_ | 1818323218473156608 |
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| author | Moon-Jin Jeong Do-Seon Lim Kyungwon Heo Soon-Jeong Jeong |
| author_facet | Moon-Jin Jeong Do-Seon Lim Kyungwon Heo Soon-Jeong Jeong |
| author_sort | Moon-Jin Jeong |
| collection | DOAJ |
| description | Background: The purpose of this study was to investigate the anti-oral microbial activity and anti-inflammatory effects of rosmarinic acid (RA) in lipopolysaccharide (LPS)-stimulated MC3T3-E1 osteoblastic cells on a titanium (Ti) surface during osseointegration, and to confirm the possibility of using RA as a safe natural substance for the control of peri-implantitis (PI) in Ti-based dental implants.Methods: A disk diffusion test was conducted to confirm the antimicrobial activity of RA against oral microorganisms. In order to confirm the anti-inflammatory effects of RA, inflammatory conditions were induced with 100 ng/ml of LPS in MC3T3-E1 osteoblastic cells on the Ti surface treated with or without 14 μg/ml of RA. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface was confirmed using an NO assay kit and PGE2 enzyme-linked immunosorbent assay kit. Reverse transcription polymerase chain reaction and western blot analysis were performed to confirm the expression of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in total RNA and protein. Results: RA showed weak antimicrobial effects against Streptococcus mutans and Escherichia coli, but no antimicrobial activity against the bacteria Aggregatibacter actinomycetemcomitans and the fungus Candida albicans. RA reduced the production of pro-inflammatory mediators, NO and PGE2, and proinflammatory cytokines, TNF-α and IL-1β, in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface at the protein and mRNA levels.Conclusion: RA not only has anti-oral microbial activity, but also anti-inflammatory effects in LPS-stimulated MC3T3-E1 osteoblasts on the Ti surface, therefore, it can be used as a safe functional substance derived from plants for the prevention and control of PI for successful Ti-based implants. |
| first_indexed | 2024-12-13T11:09:12Z |
| format | Article |
| id | doaj.art-0c4afe1e10db487e87b7ff271c57abd2 |
| institution | Directory Open Access Journal |
| issn | 2233-7679 |
| language | English |
| last_indexed | 2024-12-13T11:09:12Z |
| publishDate | 2020-12-01 |
| publisher | The Korean Society of Dental Hygiene Science |
| record_format | Article |
| series | 치위생과학회지 |
| spelling | doaj.art-0c4afe1e10db487e87b7ff271c57abd22022-12-21T23:48:48ZengThe Korean Society of Dental Hygiene Science치위생과학회지2233-76792020-12-0120422122910.17135/jdhs.2020.20.4.221Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium SurfaceMoon-Jin Jeong0https://orcid.org/0000-0002-5547-898XDo-Seon Lim1https://orcid.org/0000-0003-4602-3323Kyungwon Heo2https://orcid.org/0000-0003-0214-2216Soon-Jeong Jeong3https://orcid.org/0000-0002-8959-4663Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 61452, KoreaDepartment of Dental Hygiene, Graduate School of Public Health Science, Eulji University, Seongnam 13135, KoreaDepartment of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 61452, KoreaDepartment of Dental Hygiene & Institute of Basic Science for Well-Aging, Youngsan University, Yangsan 50510, KoreaBackground: The purpose of this study was to investigate the anti-oral microbial activity and anti-inflammatory effects of rosmarinic acid (RA) in lipopolysaccharide (LPS)-stimulated MC3T3-E1 osteoblastic cells on a titanium (Ti) surface during osseointegration, and to confirm the possibility of using RA as a safe natural substance for the control of peri-implantitis (PI) in Ti-based dental implants.Methods: A disk diffusion test was conducted to confirm the antimicrobial activity of RA against oral microorganisms. In order to confirm the anti-inflammatory effects of RA, inflammatory conditions were induced with 100 ng/ml of LPS in MC3T3-E1 osteoblastic cells on the Ti surface treated with or without 14 μg/ml of RA. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface was confirmed using an NO assay kit and PGE2 enzyme-linked immunosorbent assay kit. Reverse transcription polymerase chain reaction and western blot analysis were performed to confirm the expression of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in total RNA and protein. Results: RA showed weak antimicrobial effects against Streptococcus mutans and Escherichia coli, but no antimicrobial activity against the bacteria Aggregatibacter actinomycetemcomitans and the fungus Candida albicans. RA reduced the production of pro-inflammatory mediators, NO and PGE2, and proinflammatory cytokines, TNF-α and IL-1β, in LPS-stimulated MC3T3-E1 osteoblastic cells on the Ti surface at the protein and mRNA levels.Conclusion: RA not only has anti-oral microbial activity, but also anti-inflammatory effects in LPS-stimulated MC3T3-E1 osteoblasts on the Ti surface, therefore, it can be used as a safe functional substance derived from plants for the prevention and control of PI for successful Ti-based implants.anti-inflammatory effectanti-microbial activitymc3t3-e1 osteoblastic cellrosmarinic acidtitanium |
| spellingShingle | Moon-Jin Jeong Do-Seon Lim Kyungwon Heo Soon-Jeong Jeong Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface 치위생과학회지 anti-inflammatory effect anti-microbial activity mc3t3-e1 osteoblastic cell rosmarinic acid titanium |
| title | Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface |
| title_full | Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface |
| title_fullStr | Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface |
| title_full_unstemmed | Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface |
| title_short | Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface |
| title_sort | anti oral microbial activity and anti inflammatory effects of rosmarinic acid in lipopolysaccharide stimulated mc3t3 e1 osteoblastic cells on a titanium surface |
| topic | anti-inflammatory effect anti-microbial activity mc3t3-e1 osteoblastic cell rosmarinic acid titanium |
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