| Summary: | The paper presents the results of the studies performed to establish the effect of the mixtures between limonene and clotrimazole against microbial pathogens involved in dermatological diseases, such as <i>Candida albicans</i>, <i>Staphyloccocus aureus</i>, and <i>Escherichia coli</i>. Preliminary data obtained from the studies performed in microplates revealed a possible synergism between the mixture of clotrimazole and limonene for <i>Staphylococcus aureus</i>. Studies performed “in silico” with programs such as CLC Drug Discovery Workbench and MOLEGRO Virtual Docker, gave favorable scores for docking each compound on a specific binding site for each microorganism. The tests performed for validation, with the clotrimazole (0.1%) and different sources of limonene (1.9% citrus essential oils), showed a synergistic effect on <i>Staphylococcus aureus</i> in the case of the mixtures between clotrimazole and the essential oils of <i>Citrus reticulata</i> or <i>Citrus paradisi</i>. The studies performed on <i>Staphylococcus aureus</i> MRSA showed a synergistic effect between clotrimazole and the essential oils obtained from <i>Citrus bergamia</i>, <i>Citrus aurantium</i>, or <i>Citrus paradisi</i>. In the case of <i>Pseudomonas aeruginosa</i>, essential oils and clotrimazole used alone did not exhibit antimicrobial activities, but the mixtures between clotrimazole and the essential oils of <i>Citrus bergamia</i> or <i>Citrus sinensis</i> exhibited a synergistic antimicrobial effect.
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