miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways
Yan Yu,1,* Niu Xiang,2,* Min Lin,2 Jin-Wen Huang,2 Jing Zhang,2 Bo Cheng,2 Chao Ji2 1Department of Dermatology, First Hospital of Jilin University, Changchun, Jilin 130021, People’s Republic of China; 2Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, F...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-10-01
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| Series: | Drug Design, Development and Therapy |
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| Online Access: | https://www.dovepress.com/mir--26a-sensitizes-melanoma-cells-to-dabrafenib-via-targeting-hmgb1-d-peer-reviewed-article-DDDT |
| _version_ | 1831760598281486336 |
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| author | Yu Y Xiang N Lin M Huang JW Zhang J Cheng B Ji C |
| author_facet | Yu Y Xiang N Lin M Huang JW Zhang J Cheng B Ji C |
| author_sort | Yu Y |
| collection | DOAJ |
| description | Yan Yu,1,* Niu Xiang,2,* Min Lin,2 Jin-Wen Huang,2 Jing Zhang,2 Bo Cheng,2 Chao Ji2 1Department of Dermatology, First Hospital of Jilin University, Changchun, Jilin 130021, People’s Republic of China; 2Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chao JiDepartment of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, People’s Republic of ChinaEmail surpassing.ji@gmail.comBo ChengDepartment of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, People’s Republic of ChinaEmail chengbo_fjmu1@163.comBackground: Melanoma is known as the most aggressive and lethal type of cutaneous cancer due to its rapid development of drug resistance to chemotherapy drugs.Methods: In our study, we conducted a variety of studies, including quantitative PCR, Western blot, and autophagy and apoptosis assays to investigate the involvement of miR-26a and HMGB1 in modulation of dabrafenib sensitivity in human melanoma cell lines.Results: Our studies revealed that the expressions of miR-26a and HMGB1 were altered in two melanoma cell lines after dabrafenib treatment. Additionally, dabrafenib caused autophagy in melanoma and this autophagic process was regulated by miR-26a via modifying HMGB1 expression. Furthermore, silencing HMGB1-inhibited autophagy induced by dabrafenib in melanoma cells. Last, we verified that treatment with a miR-26a mimic and HMGB1 shRNA could increase the efficacy of dabrafenib in melanoma cells.Conclusion: Taken together, we showed that miR-26a is involved in the regulation of dabrafenib efficacy via a HMGB1-dependent autophagy pathway in melanoma cells. These results shed light on a novel treatment for conventional dabrafenib-based chemotherapy for melanoma.Keywords: melanoma, miR-26a, HMGB1, dabrafenib, autophagy, apoptosis |
| first_indexed | 2024-12-22T04:33:12Z |
| format | Article |
| id | doaj.art-0c4e62bf46944701866037156d47f1e6 |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-22T04:33:12Z |
| publishDate | 2019-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-0c4e62bf46944701866037156d47f1e62022-12-21T18:38:57ZengDove Medical PressDrug Design, Development and Therapy1177-88812019-10-01Volume 133717372649359miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy PathwaysYu YXiang NLin MHuang JWZhang JCheng BJi CYan Yu,1,* Niu Xiang,2,* Min Lin,2 Jin-Wen Huang,2 Jing Zhang,2 Bo Cheng,2 Chao Ji2 1Department of Dermatology, First Hospital of Jilin University, Changchun, Jilin 130021, People’s Republic of China; 2Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chao JiDepartment of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, People’s Republic of ChinaEmail surpassing.ji@gmail.comBo ChengDepartment of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, People’s Republic of ChinaEmail chengbo_fjmu1@163.comBackground: Melanoma is known as the most aggressive and lethal type of cutaneous cancer due to its rapid development of drug resistance to chemotherapy drugs.Methods: In our study, we conducted a variety of studies, including quantitative PCR, Western blot, and autophagy and apoptosis assays to investigate the involvement of miR-26a and HMGB1 in modulation of dabrafenib sensitivity in human melanoma cell lines.Results: Our studies revealed that the expressions of miR-26a and HMGB1 were altered in two melanoma cell lines after dabrafenib treatment. Additionally, dabrafenib caused autophagy in melanoma and this autophagic process was regulated by miR-26a via modifying HMGB1 expression. Furthermore, silencing HMGB1-inhibited autophagy induced by dabrafenib in melanoma cells. Last, we verified that treatment with a miR-26a mimic and HMGB1 shRNA could increase the efficacy of dabrafenib in melanoma cells.Conclusion: Taken together, we showed that miR-26a is involved in the regulation of dabrafenib efficacy via a HMGB1-dependent autophagy pathway in melanoma cells. These results shed light on a novel treatment for conventional dabrafenib-based chemotherapy for melanoma.Keywords: melanoma, miR-26a, HMGB1, dabrafenib, autophagy, apoptosishttps://www.dovepress.com/mir--26a-sensitizes-melanoma-cells-to-dabrafenib-via-targeting-hmgb1-d-peer-reviewed-article-DDDTmelanomamir-26ahmgb1dabrafenibautophagyapoptosis |
| spellingShingle | Yu Y Xiang N Lin M Huang JW Zhang J Cheng B Ji C miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways Drug Design, Development and Therapy melanoma mir-26a hmgb1 dabrafenib autophagy apoptosis |
| title | miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways |
| title_full | miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways |
| title_fullStr | miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways |
| title_full_unstemmed | miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways |
| title_short | miR- 26a Sensitizes Melanoma Cells To Dabrafenib Via Targeting HMGB1-Dependent Autophagy Pathways |
| title_sort | mir 26a sensitizes melanoma cells to dabrafenib via targeting hmgb1 dependent autophagy pathways |
| topic | melanoma mir-26a hmgb1 dabrafenib autophagy apoptosis |
| url | https://www.dovepress.com/mir--26a-sensitizes-melanoma-cells-to-dabrafenib-via-targeting-hmgb1-d-peer-reviewed-article-DDDT |
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