Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA
Medium chain esters produced from fruits and flowering plants have a number of commercial applications including use as flavour and fragrance ingredients, biofuels, and in pharmaceutical formulations. These esters are typically made via the activity of an alcohol acyl transferase (AAT) enzyme which...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2019-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/6971.pdf |
| _version_ | 1797418228403666944 |
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| author | Micaela G. Chacón Emanuele G. Kendrick David J. Leak |
| author_facet | Micaela G. Chacón Emanuele G. Kendrick David J. Leak |
| author_sort | Micaela G. Chacón |
| collection | DOAJ |
| description | Medium chain esters produced from fruits and flowering plants have a number of commercial applications including use as flavour and fragrance ingredients, biofuels, and in pharmaceutical formulations. These esters are typically made via the activity of an alcohol acyl transferase (AAT) enzyme which catalyses the condensation of an alcohol and an acyl-CoA. Developing a microbial platform for medium chain ester production using AAT activity presents several obstacles, including the low product specificity of these enzymes for the desired ester and/or low endogenous substrate availability. In this study, we engineered Escherichia coli for the production of butyl octanoate from endogenously produced octanoyl-CoA. This was achieved through rational protein engineering of an AAT enzyme from Actinidia chinensis for improved octanoyl-CoA substrate specificity and metabolic engineering of E. coli fatty acid metabolism for increased endogenous octanoyl-CoA availability. This resulted in accumulation of 3.3 + 0.1 mg/L butyl octanoate as the sole product from E. coli after 48 h. This study represents a preliminary examination of the feasibility of developing E. coli platforms for the synthesis single medium chain esters from endogenous fatty acids. |
| first_indexed | 2024-03-09T06:30:40Z |
| format | Article |
| id | doaj.art-0c51666e92a3495d84d9092789700da9 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:30:40Z |
| publishDate | 2019-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-0c51666e92a3495d84d9092789700da92023-12-03T11:05:40ZengPeerJ Inc.PeerJ2167-83592019-07-017e697110.7717/peerj.6971Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoAMicaela G. ChacónEmanuele G. KendrickDavid J. LeakMedium chain esters produced from fruits and flowering plants have a number of commercial applications including use as flavour and fragrance ingredients, biofuels, and in pharmaceutical formulations. These esters are typically made via the activity of an alcohol acyl transferase (AAT) enzyme which catalyses the condensation of an alcohol and an acyl-CoA. Developing a microbial platform for medium chain ester production using AAT activity presents several obstacles, including the low product specificity of these enzymes for the desired ester and/or low endogenous substrate availability. In this study, we engineered Escherichia coli for the production of butyl octanoate from endogenously produced octanoyl-CoA. This was achieved through rational protein engineering of an AAT enzyme from Actinidia chinensis for improved octanoyl-CoA substrate specificity and metabolic engineering of E. coli fatty acid metabolism for increased endogenous octanoyl-CoA availability. This resulted in accumulation of 3.3 + 0.1 mg/L butyl octanoate as the sole product from E. coli after 48 h. This study represents a preliminary examination of the feasibility of developing E. coli platforms for the synthesis single medium chain esters from endogenous fatty acids.https://peerj.com/articles/6971.pdfButyl octanoateAlcohol acyltranferaseMetabolic engineeringMicrobial productionFatty acid metabolism |
| spellingShingle | Micaela G. Chacón Emanuele G. Kendrick David J. Leak Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA PeerJ Butyl octanoate Alcohol acyltranferase Metabolic engineering Microbial production Fatty acid metabolism |
| title | Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA |
| title_full | Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA |
| title_fullStr | Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA |
| title_full_unstemmed | Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA |
| title_short | Engineering Escherichia coli for the production of butyl octanoate from endogenous octanoyl-CoA |
| title_sort | engineering escherichia coli for the production of butyl octanoate from endogenous octanoyl coa |
| topic | Butyl octanoate Alcohol acyltranferase Metabolic engineering Microbial production Fatty acid metabolism |
| url | https://peerj.com/articles/6971.pdf |
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