Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i>
Bumble bees are key pollinators for wild and managed plants and serve as a model system in various research fields, largely due to their commercial availability. Despite their extensive use, laboratory rearing of bumble bees is often challenging, particularly during the solitary phase queens undergo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/8/673 |
| _version_ | 1797523411576029184 |
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| author | Erin Treanore Katherine Barie Nathan Derstine Kaitlin Gadebusch Margarita Orlova Monique Porter Frederick Purnell Etya Amsalem |
| author_facet | Erin Treanore Katherine Barie Nathan Derstine Kaitlin Gadebusch Margarita Orlova Monique Porter Frederick Purnell Etya Amsalem |
| author_sort | Erin Treanore |
| collection | DOAJ |
| description | Bumble bees are key pollinators for wild and managed plants and serve as a model system in various research fields, largely due to their commercial availability. Despite their extensive use, laboratory rearing of bumble bees is often challenging, particularly during the solitary phase queens undergo before founding a colony. Using a literature survey, we demonstrate that most studies rely on commercially available species that are provided during the colony’s social phase, limiting study on early phases of the life cycle and the ability to control for colony age and relatedness. Laboratory rearing is challenging since the queen solitary phase is less understood compared to the social phase. To overcome this barrier, we examined several aspects related to the queen solitary phase: the effect of age on likelihood of mating, how the timing of CO<sub>2</sub> narcosis post-mating (a technique to bypass diapause) affects egg-laying, and whether different social cues affect the success of colony initiation. Our data show an optimum age for mating in both sexuals and decreased egg-laying latency in the presence of workers and pupae. The timing of CO<sub>2</sub> narcosis did not significantly affect egg laying in queens. These findings can be incorporated to improve bumble bee rearing for research purposes. |
| first_indexed | 2024-03-10T08:42:34Z |
| format | Article |
| id | doaj.art-0c516e9f0ca0403bbf10ebbce3eadd9f |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T08:42:34Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-0c516e9f0ca0403bbf10ebbce3eadd9f2023-11-22T08:06:50ZengMDPI AGInsects2075-44502021-07-0112867310.3390/insects12080673Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i>Erin Treanore0Katherine Barie1Nathan Derstine2Kaitlin Gadebusch3Margarita Orlova4Monique Porter5Frederick Purnell6Etya Amsalem7Department of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USADepartment of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USADepartment of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USADepartment of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USADepartment of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USADepartment of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USADepartment of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USADepartment of Entomology, Center for Chemical Ecology, Center for Pollinator Research, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USABumble bees are key pollinators for wild and managed plants and serve as a model system in various research fields, largely due to their commercial availability. Despite their extensive use, laboratory rearing of bumble bees is often challenging, particularly during the solitary phase queens undergo before founding a colony. Using a literature survey, we demonstrate that most studies rely on commercially available species that are provided during the colony’s social phase, limiting study on early phases of the life cycle and the ability to control for colony age and relatedness. Laboratory rearing is challenging since the queen solitary phase is less understood compared to the social phase. To overcome this barrier, we examined several aspects related to the queen solitary phase: the effect of age on likelihood of mating, how the timing of CO<sub>2</sub> narcosis post-mating (a technique to bypass diapause) affects egg-laying, and whether different social cues affect the success of colony initiation. Our data show an optimum age for mating in both sexuals and decreased egg-laying latency in the presence of workers and pupae. The timing of CO<sub>2</sub> narcosis did not significantly affect egg laying in queens. These findings can be incorporated to improve bumble bee rearing for research purposes.https://www.mdpi.com/2075-4450/12/8/673bumble beesmatingCO<sub>2</sub> narcosissocial insectssocial cuessolitary phase |
| spellingShingle | Erin Treanore Katherine Barie Nathan Derstine Kaitlin Gadebusch Margarita Orlova Monique Porter Frederick Purnell Etya Amsalem Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i> Insects bumble bees mating CO<sub>2</sub> narcosis social insects social cues solitary phase |
| title | Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i> |
| title_full | Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i> |
| title_fullStr | Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i> |
| title_full_unstemmed | Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i> |
| title_short | Optimizing Laboratory Rearing of a Key Pollinator, <i>Bombus impatiens</i> |
| title_sort | optimizing laboratory rearing of a key pollinator i bombus impatiens i |
| topic | bumble bees mating CO<sub>2</sub> narcosis social insects social cues solitary phase |
| url | https://www.mdpi.com/2075-4450/12/8/673 |
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