Postconditioning attenuates acute intestinal ischemia–reperfusion injury
The aim of this study was to test the hypothesis that postconditioning (POC) would reduce the detrimental effects of the acute intestinal ischemia–reperfusion (I/R) compared to those of the abrupt onset of reperfusion. POC has a protective effect on intestinal I/R injury by inhibiting events in the...
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| Format: | Article |
| Language: | English |
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Wiley
2013-03-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X12002227 |
| _version_ | 1831724368335470592 |
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| author | Ilker Sengul Demet Sengul Osman Guler Adnan Hasanoglu Mustafa Kemal Urhan Ahmet Sukru Taner Jakob Vinten-Johansen |
| author_facet | Ilker Sengul Demet Sengul Osman Guler Adnan Hasanoglu Mustafa Kemal Urhan Ahmet Sukru Taner Jakob Vinten-Johansen |
| author_sort | Ilker Sengul |
| collection | DOAJ |
| description | The aim of this study was to test the hypothesis that postconditioning (POC) would reduce the detrimental effects of the acute intestinal ischemia–reperfusion (I/R) compared to those of the abrupt onset of reperfusion. POC has a protective effect on intestinal I/R injury by inhibiting events in the early minutes of reperfusion in rats. Twenty-four Wistar–Albino rats were subjected to the occlusion of superior mesenteric artery for 30 minutes, then reperfused for 120 minutes, and randomized to the four different modalities of POC: (1) control (no intervention); (2) POC-3 (three cycles of 10 seconds of reperfusion–reocclusion, 1 minute total intervention); (3) POC-6 (six cycles of 10 seconds of reperfusion–reocclusion, 2 minutes total intervention); and (4) sham operation (laparotomy only). The arterial blood samples [0.3 mL total creatine kinase (CK) and 0.6 mL malondialdehyde (MDA)] and the intestinal mucosal MDA were collected from each after reperfusion. POC, especially POC-6, was effective in attenuating postischemic pathology by decreasing the intestinal tissue MDA levels, serum total CK activity, inflammation, and total histopathological injury scores. POC exerted a protective effect on the intestinal mucosa by reducing the mesenteric oxidant generation, lipid peroxidation, and neutrophil accumulation. The six-cycle algorithm demonstrated the best protection. |
| first_indexed | 2024-12-21T04:20:38Z |
| format | Article |
| id | doaj.art-0c5292d927b343f6b94b9ec933c6fbf7 |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-12-21T04:20:38Z |
| publishDate | 2013-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-0c5292d927b343f6b94b9ec933c6fbf72022-12-21T19:16:11ZengWileyKaohsiung Journal of Medical Sciences1607-551X2013-03-0129311912710.1016/j.kjms.2012.08.021Postconditioning attenuates acute intestinal ischemia–reperfusion injuryIlker Sengul0Demet Sengul1Osman Guler2Adnan Hasanoglu3Mustafa Kemal Urhan4Ahmet Sukru Taner5Jakob Vinten-Johansen6Department of General Surgery, Giresun University Faculty of Medicine, Giresun, TurkeyDepartment of Pathology, Giresun University Faculty of Medicine, Giresun, TurkeyDepartment of 1st General Surgery, Ankara Education and Research Hospital, Ankara, TurkeyDepartment of 1st General Surgery, Ankara Education and Research Hospital, Ankara, TurkeyDepartment of 1st General Surgery, Ankara Education and Research Hospital, Ankara, TurkeyDepartment of 1st General Surgery, Ankara Education and Research Hospital, Ankara, TurkeyDepartment of Surgery (Cardiothoracic), Cardiothoracic Research Laboratory of Emory University, Carlyle Fraser Heart Center, Atlanta, GA, USAThe aim of this study was to test the hypothesis that postconditioning (POC) would reduce the detrimental effects of the acute intestinal ischemia–reperfusion (I/R) compared to those of the abrupt onset of reperfusion. POC has a protective effect on intestinal I/R injury by inhibiting events in the early minutes of reperfusion in rats. Twenty-four Wistar–Albino rats were subjected to the occlusion of superior mesenteric artery for 30 minutes, then reperfused for 120 minutes, and randomized to the four different modalities of POC: (1) control (no intervention); (2) POC-3 (three cycles of 10 seconds of reperfusion–reocclusion, 1 minute total intervention); (3) POC-6 (six cycles of 10 seconds of reperfusion–reocclusion, 2 minutes total intervention); and (4) sham operation (laparotomy only). The arterial blood samples [0.3 mL total creatine kinase (CK) and 0.6 mL malondialdehyde (MDA)] and the intestinal mucosal MDA were collected from each after reperfusion. POC, especially POC-6, was effective in attenuating postischemic pathology by decreasing the intestinal tissue MDA levels, serum total CK activity, inflammation, and total histopathological injury scores. POC exerted a protective effect on the intestinal mucosa by reducing the mesenteric oxidant generation, lipid peroxidation, and neutrophil accumulation. The six-cycle algorithm demonstrated the best protection.http://www.sciencedirect.com/science/article/pii/S1607551X12002227Creatine kinaseIntestinal ischemia–reperfusionIschemia–reperfusion injuryMalondialdehydePostconditioning |
| spellingShingle | Ilker Sengul Demet Sengul Osman Guler Adnan Hasanoglu Mustafa Kemal Urhan Ahmet Sukru Taner Jakob Vinten-Johansen Postconditioning attenuates acute intestinal ischemia–reperfusion injury Kaohsiung Journal of Medical Sciences Creatine kinase Intestinal ischemia–reperfusion Ischemia–reperfusion injury Malondialdehyde Postconditioning |
| title | Postconditioning attenuates acute intestinal ischemia–reperfusion injury |
| title_full | Postconditioning attenuates acute intestinal ischemia–reperfusion injury |
| title_fullStr | Postconditioning attenuates acute intestinal ischemia–reperfusion injury |
| title_full_unstemmed | Postconditioning attenuates acute intestinal ischemia–reperfusion injury |
| title_short | Postconditioning attenuates acute intestinal ischemia–reperfusion injury |
| title_sort | postconditioning attenuates acute intestinal ischemia reperfusion injury |
| topic | Creatine kinase Intestinal ischemia–reperfusion Ischemia–reperfusion injury Malondialdehyde Postconditioning |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X12002227 |
| work_keys_str_mv | AT ilkersengul postconditioningattenuatesacuteintestinalischemiareperfusioninjury AT demetsengul postconditioningattenuatesacuteintestinalischemiareperfusioninjury AT osmanguler postconditioningattenuatesacuteintestinalischemiareperfusioninjury AT adnanhasanoglu postconditioningattenuatesacuteintestinalischemiareperfusioninjury AT mustafakemalurhan postconditioningattenuatesacuteintestinalischemiareperfusioninjury AT ahmetsukrutaner postconditioningattenuatesacuteintestinalischemiareperfusioninjury AT jakobvintenjohansen postconditioningattenuatesacuteintestinalischemiareperfusioninjury |