Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying Hens
The principal purpose of this research was to study the effects of glycerol monolaurate (GML) on the production performance; egg quality; health state of the oviduct, ovary and ileum; and gut microbiota of laying hens in the later stage. The laying hens were randomly assigned to two groups: a contro...
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MDPI AG
2023-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/2/215 |
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| author | Zhenchuan Cui Ruiqiang Zhang Bing Dai Chunsheng Fu Guiling Zhao Yinglei Xu Caimei Yang |
| author_facet | Zhenchuan Cui Ruiqiang Zhang Bing Dai Chunsheng Fu Guiling Zhao Yinglei Xu Caimei Yang |
| author_sort | Zhenchuan Cui |
| collection | DOAJ |
| description | The principal purpose of this research was to study the effects of glycerol monolaurate (GML) on the production performance; egg quality; health state of the oviduct, ovary and ileum; and gut microbiota of laying hens in the later stage. The laying hens were randomly assigned to two groups: a control group and an experiment group, for which 1000 mg/kg of GML was added to a control diet. The results showed that GML increased the laying rate, average egg weight, albumen height, yolk color and Haugh unit and decreased the feed conversion ratio and defective eggs (<i>p</i> < 0.05). GML increased the intestinal villi height and the ratio of villus height to crypt depth (<i>p</i> < 0.05). Moreover, GML improved the contents of cytokines in the oviduct, ovary and ileum mucosa; ameliorated the expression of <i>TLR2</i>, <i>TLR4</i>, <i>MyD88</i>, <i>IL-4</i>, <i>IL-1β</i> and <i>TNF-α</i>; and increased the expression of <i>Occludin</i> and <i>Muc-2</i> in the ileal mucosa. The supplementation of GML increased the volatile fatty acids in the cecal contents, such as acetic acid and propionic acid, and up-regulated <i>Bacteroides</i> (<i>p</i> < 0.01) and <i>Alistipes</i> (<i>p</i> < 0.05) richness in the cecal contents. In summary, GML improved production performance, egg quality and immunity; ameliorated the health status of the oviduct, ovary and ileum; enhanced the intestinal barrier function; improved the content of intestinal volatile fatty acids; and regulated the abundance of cecal flora. |
| first_indexed | 2024-03-09T13:48:44Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T13:48:44Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-0c52e3b1583f495ebbc47d67020acbe72023-11-30T20:52:49ZengMDPI AGAnimals2076-26152023-01-0113221510.3390/ani13020215Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying HensZhenchuan Cui0Ruiqiang Zhang1Bing Dai2Chunsheng Fu3Guiling Zhao4Yinglei Xu5Caimei Yang6College of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, ChinaCollege of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, ChinaCollege of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, ChinaCollege of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, ChinaCollege of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, ChinaCollege of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, ChinaCollege of Animal Science and Technology, College of Animal Medicine, Zhejiang Agriculture & Forestry University, Hangzhou 311300, ChinaThe principal purpose of this research was to study the effects of glycerol monolaurate (GML) on the production performance; egg quality; health state of the oviduct, ovary and ileum; and gut microbiota of laying hens in the later stage. The laying hens were randomly assigned to two groups: a control group and an experiment group, for which 1000 mg/kg of GML was added to a control diet. The results showed that GML increased the laying rate, average egg weight, albumen height, yolk color and Haugh unit and decreased the feed conversion ratio and defective eggs (<i>p</i> < 0.05). GML increased the intestinal villi height and the ratio of villus height to crypt depth (<i>p</i> < 0.05). Moreover, GML improved the contents of cytokines in the oviduct, ovary and ileum mucosa; ameliorated the expression of <i>TLR2</i>, <i>TLR4</i>, <i>MyD88</i>, <i>IL-4</i>, <i>IL-1β</i> and <i>TNF-α</i>; and increased the expression of <i>Occludin</i> and <i>Muc-2</i> in the ileal mucosa. The supplementation of GML increased the volatile fatty acids in the cecal contents, such as acetic acid and propionic acid, and up-regulated <i>Bacteroides</i> (<i>p</i> < 0.01) and <i>Alistipes</i> (<i>p</i> < 0.05) richness in the cecal contents. In summary, GML improved production performance, egg quality and immunity; ameliorated the health status of the oviduct, ovary and ileum; enhanced the intestinal barrier function; improved the content of intestinal volatile fatty acids; and regulated the abundance of cecal flora.https://www.mdpi.com/2076-2615/13/2/215glycerol monolauratelaying rateegg qualityoviduct cytokinesintestinal villi morphologyintestinal microflora |
| spellingShingle | Zhenchuan Cui Ruiqiang Zhang Bing Dai Chunsheng Fu Guiling Zhao Yinglei Xu Caimei Yang Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying Hens Animals glycerol monolaurate laying rate egg quality oviduct cytokines intestinal villi morphology intestinal microflora |
| title | Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying Hens |
| title_full | Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying Hens |
| title_fullStr | Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying Hens |
| title_full_unstemmed | Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying Hens |
| title_short | Effects of Glyceryl Monolaurate on Production Performance, Egg Quality, Oviduct Cytokines and Intestinal Microflora of 66 Weeks Old Laying Hens |
| title_sort | effects of glyceryl monolaurate on production performance egg quality oviduct cytokines and intestinal microflora of 66 weeks old laying hens |
| topic | glycerol monolaurate laying rate egg quality oviduct cytokines intestinal villi morphology intestinal microflora |
| url | https://www.mdpi.com/2076-2615/13/2/215 |
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