mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal Cancer
Colorectal cancer is a major global health problem. The aim of the current study is to determine the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase -1 (MMP-1), in colorectal cancer patients using in situ hybridization technique, formalin-fixed and paraffin embe...
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2016-12-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
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| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/111 |
| _version_ | 1797223901459120128 |
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| author | Noor Hashim Ismail |
| author_facet | Noor Hashim Ismail |
| author_sort | Noor Hashim Ismail |
| collection | DOAJ |
| description |
Colorectal cancer is a major global health problem. The aim of the current study is to determine the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase -1 (MMP-1), in colorectal cancer patients using in situ hybridization technique, formalin-fixed and paraffin embedded tissue sections.
Thirty colorectal cancer patients were obtained from the archive of the pathology laboratory of Al-Yarmouk Teaching Hospital from January 2013 to July 2014. In addition to, formalin-fixed, paraffin embedded tissue sections of twenty normal colon tissues were collected and used as a control group. Each tissue block was subjected to cut as serial thin sections of (4μm) thickness and then fixed on positive charged slides, to be used for In situ hybridization for the evaluation of MMP-1 and VEGF expression.
A highly significant difference (p<0.01) was noted in the mean percentage of MMP-1 and VEGF mRNA in situ expression respectively between the colorectal cancer group and control group. The study revealed a highly significant positive correlation (P<0.01) between MMP-1 and VEGF expression in colorectal cancer group. In conclusion, abnormal high expressions of VEGF and MMP-1 may represent the early molecular changes in the development of colorectal cancer
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| first_indexed | 2024-04-24T13:44:35Z |
| format | Article |
| id | doaj.art-0c53f066f85743d29481ef07185b629b |
| institution | Directory Open Access Journal |
| issn | 1815-0993 2959-183X |
| language | English |
| last_indexed | 2024-04-24T13:44:35Z |
| publishDate | 2016-12-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj.art-0c53f066f85743d29481ef07185b629b2024-04-04T06:58:17ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2016-12-0116210.32947/ajps.v16i2.111mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal CancerNoor Hashim Ismail0Department of Cancer Research, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq. Colorectal cancer is a major global health problem. The aim of the current study is to determine the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase -1 (MMP-1), in colorectal cancer patients using in situ hybridization technique, formalin-fixed and paraffin embedded tissue sections. Thirty colorectal cancer patients were obtained from the archive of the pathology laboratory of Al-Yarmouk Teaching Hospital from January 2013 to July 2014. In addition to, formalin-fixed, paraffin embedded tissue sections of twenty normal colon tissues were collected and used as a control group. Each tissue block was subjected to cut as serial thin sections of (4μm) thickness and then fixed on positive charged slides, to be used for In situ hybridization for the evaluation of MMP-1 and VEGF expression. A highly significant difference (p<0.01) was noted in the mean percentage of MMP-1 and VEGF mRNA in situ expression respectively between the colorectal cancer group and control group. The study revealed a highly significant positive correlation (P<0.01) between MMP-1 and VEGF expression in colorectal cancer group. In conclusion, abnormal high expressions of VEGF and MMP-1 may represent the early molecular changes in the development of colorectal cancer https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/111Colorectal cancer, in situ hybridization, vascular endothelial growth factor, matrix metalloproteinase -1. |
| spellingShingle | Noor Hashim Ismail mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal Cancer Al-Mustansiriyah Journal of Pharmaceutical Sciences Colorectal cancer, in situ hybridization, vascular endothelial growth factor, matrix metalloproteinase -1. |
| title | mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal Cancer |
| title_full | mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal Cancer |
| title_fullStr | mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal Cancer |
| title_full_unstemmed | mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal Cancer |
| title_short | mRNA in situ Hybridization Analysis of Vascular Endothelial Growth Factor and Matrix Metalloproteinase-1 in Colorectal Cancer |
| title_sort | mrna in situ hybridization analysis of vascular endothelial growth factor and matrix metalloproteinase 1 in colorectal cancer |
| topic | Colorectal cancer, in situ hybridization, vascular endothelial growth factor, matrix metalloproteinase -1. |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/111 |
| work_keys_str_mv | AT noorhashimismail mrnainsituhybridizationanalysisofvascularendothelialgrowthfactorandmatrixmetalloproteinase1incolorectalcancer |