Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo Studies
<i>Litsea glutinosa</i> (<i>L. glutinosa</i>) is considered an evidence-based medicinal plant for the treatment of cancer, the leading cause of death worldwide. In our study, the in vitro antioxidant and in vivo anticancer properties of an essential ethno-medicinal plant, <...
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| author | Shafia Shafiq Ronok Zahan Samina Yesmin Alam Khan Md. Sabbir Mahmud Md Abu Reza Sarah M. Albogami Mohammed Alorabi Michel De Waard Hebatallah M. Saad Jean-Marc Sabatier Tarannum Naz Gaber El-Saber Batiha |
| author_facet | Shafia Shafiq Ronok Zahan Samina Yesmin Alam Khan Md. Sabbir Mahmud Md Abu Reza Sarah M. Albogami Mohammed Alorabi Michel De Waard Hebatallah M. Saad Jean-Marc Sabatier Tarannum Naz Gaber El-Saber Batiha |
| author_sort | Shafia Shafiq |
| collection | DOAJ |
| description | <i>Litsea glutinosa</i> (<i>L. glutinosa</i>) is considered an evidence-based medicinal plant for the treatment of cancer, the leading cause of death worldwide. In our study, the in vitro antioxidant and in vivo anticancer properties of an essential ethno-medicinal plant, <i>L. glutinosa</i>, were examined using non-toxic doses and a phytochemical analysis was executed using gas-chromatography–mass-spectrometry. The in vitro antioxidant study of the <i>L. glutinosa</i> methanolic extract (LGBME) revealed a concentration-dependent antioxidant property. The bark extract showed promising antioxidant effects in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. The strongest antioxidant activity was demonstrated at the maximum concentration (50 µg/mL). The IC<sub>50</sub> values of the LGBME and BHT were 5.51 and 5.01 µg/mL, respectively. At the same concentration, the total antioxidant capacity of the LGBME was 0.161 µg/mL and the ferric reducing antioxidant power assay result of the LGBME was 1.783 µg/mL. In the cytotoxicity study, the LD<sub>50</sub> of the LGBME and gallic acid were 24.93 µg/mL and 7.23 µg/mL, respectively. In the in vivo anticancer-activity studies, the LGBME, particularly at a dose of 150 mg/kg/bw, showed significant cell-growth inhibition, decreased tumor weight, increased mean survival rate, and upregulated the reduced hematological parameters in EAC (Ehrlich’s ascites carcinoma)-induced Swiss albino mice. The highest cell-growth inhibition, 85.76%, was observed with the dose of 150 mg/kg/bw. Furthermore, the upregulation of pro-apoptotic genes (p53, Bax) and the downregulation of anti-apoptotic Bcl-2 were observed. In conclusion, LGBME extract has several bioactive phytoconstituents, which confirms the antioxidant and anticancer properties of <i>L. glutinosa.</i> |
| first_indexed | 2024-03-09T19:42:03Z |
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| id | doaj.art-0c602a69c8f74007ae9c2389ca3260fc |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T19:42:03Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-0c602a69c8f74007ae9c2389ca3260fc2023-11-24T01:34:41ZengMDPI AGMolecules1420-30492022-10-012720696410.3390/molecules27206964Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo StudiesShafia Shafiq0Ronok Zahan1Samina Yesmin2Alam Khan3Md. Sabbir Mahmud4Md Abu Reza5Sarah M. Albogami6Mohammed Alorabi7Michel De Waard8Hebatallah M. Saad9Jean-Marc Sabatier10Tarannum Naz11Gaber El-Saber Batiha12Department of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaSmartox Biotechnology, 6 rue des Platanes, 38120 Saint-Egrève, FranceDepartment of Pathology, Faculty of Veterinary Medicine, Matrouh University, Mersa Matruh 51744, EgyptInstitut de Neurophysiopathologie (INP), CNRS UMR 7051, Faculté des Scienes Médicales et Paramédicales, Aix-Marseille Université, 27 Bd Jean Moulin, 13005 Marseille, FranceDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt<i>Litsea glutinosa</i> (<i>L. glutinosa</i>) is considered an evidence-based medicinal plant for the treatment of cancer, the leading cause of death worldwide. In our study, the in vitro antioxidant and in vivo anticancer properties of an essential ethno-medicinal plant, <i>L. glutinosa</i>, were examined using non-toxic doses and a phytochemical analysis was executed using gas-chromatography–mass-spectrometry. The in vitro antioxidant study of the <i>L. glutinosa</i> methanolic extract (LGBME) revealed a concentration-dependent antioxidant property. The bark extract showed promising antioxidant effects in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. The strongest antioxidant activity was demonstrated at the maximum concentration (50 µg/mL). The IC<sub>50</sub> values of the LGBME and BHT were 5.51 and 5.01 µg/mL, respectively. At the same concentration, the total antioxidant capacity of the LGBME was 0.161 µg/mL and the ferric reducing antioxidant power assay result of the LGBME was 1.783 µg/mL. In the cytotoxicity study, the LD<sub>50</sub> of the LGBME and gallic acid were 24.93 µg/mL and 7.23 µg/mL, respectively. In the in vivo anticancer-activity studies, the LGBME, particularly at a dose of 150 mg/kg/bw, showed significant cell-growth inhibition, decreased tumor weight, increased mean survival rate, and upregulated the reduced hematological parameters in EAC (Ehrlich’s ascites carcinoma)-induced Swiss albino mice. The highest cell-growth inhibition, 85.76%, was observed with the dose of 150 mg/kg/bw. Furthermore, the upregulation of pro-apoptotic genes (p53, Bax) and the downregulation of anti-apoptotic Bcl-2 were observed. In conclusion, LGBME extract has several bioactive phytoconstituents, which confirms the antioxidant and anticancer properties of <i>L. glutinosa.</i>https://www.mdpi.com/1420-3049/27/20/6964antioxidantDPPHanticancerapoptosisgas-chromatography–mass-spectrometryethnomedicinal plants |
| spellingShingle | Shafia Shafiq Ronok Zahan Samina Yesmin Alam Khan Md. Sabbir Mahmud Md Abu Reza Sarah M. Albogami Mohammed Alorabi Michel De Waard Hebatallah M. Saad Jean-Marc Sabatier Tarannum Naz Gaber El-Saber Batiha Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo Studies Molecules antioxidant DPPH anticancer apoptosis gas-chromatography–mass-spectrometry ethnomedicinal plants |
| title | Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo Studies |
| title_full | Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo Studies |
| title_fullStr | Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo Studies |
| title_full_unstemmed | Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo Studies |
| title_short | Phytochemical Analysis and Understanding the Antioxidant and Anticancer Properties of Methanol Extract from <i>Litsea glutinosa</i>: In Vitro and In Vivo Studies |
| title_sort | phytochemical analysis and understanding the antioxidant and anticancer properties of methanol extract from i litsea glutinosa i in vitro and in vivo studies |
| topic | antioxidant DPPH anticancer apoptosis gas-chromatography–mass-spectrometry ethnomedicinal plants |
| url | https://www.mdpi.com/1420-3049/27/20/6964 |
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