Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. Extract
Despite the fact that several analytical methodologies have been reported for the determination of curcumin (CCM) in a wide range of sample matrices, the greener liquid chromatographic approaches to determine CCM are scarce in the literature. Therefore, this research is designed to develop and valid...
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MDPI AG
2023-02-01
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| author | Nazrul Haq Faiyaz Shakeel Mohammed M. Ghoneim Syed Mohammed Basheeruddin Asdaq Prawez Alam Saleh A. Alanazi Sultan Alshehri |
| author_facet | Nazrul Haq Faiyaz Shakeel Mohammed M. Ghoneim Syed Mohammed Basheeruddin Asdaq Prawez Alam Saleh A. Alanazi Sultan Alshehri |
| author_sort | Nazrul Haq |
| collection | DOAJ |
| description | Despite the fact that several analytical methodologies have been reported for the determination of curcumin (CCM) in a wide range of sample matrices, the greener liquid chromatographic approaches to determine CCM are scarce in the literature. Therefore, this research is designed to develop and validate a greener stability-indicating “high-performance liquid chromatography (HPLC)” methodology to determine CCM in an in-house developed nanoemulsion, <i>Curcuma longa</i> L. extract, and commercial tablets. CCM was measured on a Nucleodur (150 mm × 4.6 mm) RP C<sub>18</sub> column with 5 µm-sized particles. Ethanol and ethyl acetate (83:17 <i>v/v</i>) made up the greener eluent system, which was pumped at a flow speed of 1.0 mL/min. At a wavelength of 425 nm, CCM was detected. The greener HPLC methodology was linear in the 1–100 µg/mL range, with a determination coefficient of 0.9983. The greener HPLC methodology for CCM estimation was also rapid (R<sub>t</sub> = 3.57 min), accurate (%recoveries = 98.90–101.85), precise (%CV = 0.90–1.11), and sensitive (LOD = 0.39 µg/mL and LOQ = 1.17 µg/mL). The AGREE approach predicted the AGREE score of 0.81 for the established HPLC technique, indicating an outstanding greenness profile. The utility of the greener HPLC methodology was demonstrated by determining CCM in the in-house developed nanoemulsion, <i>Curcuma longa</i> extract, and commercial tablets. The % amount of CCM in the in-house developed nanoemulsion, <i>Curcuma longa</i> extract, and commercial tablets was found to be 101.24%, 81.15%, and 78.41%, respectively. The greener HPLC methodology was able to detect its degradation product under various stress conditions, suggesting its stability-indication characteristics. These results suggested that CCM in developed nanoemulsion, plant extract samples, and commercial tablets may be routinely determined using the greener HPLC methodology. |
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| language | English |
| last_indexed | 2024-03-11T08:09:31Z |
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| spelling | doaj.art-0c675c1cbd28498fb8ecbd003710b4b32023-11-16T23:14:19ZengMDPI AGSeparations2297-87392023-02-011029810.3390/separations10020098Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. ExtractNazrul Haq0Faiyaz Shakeel1Mohammed M. Ghoneim2Syed Mohammed Basheeruddin Asdaq3Prawez Alam4Saleh A. Alanazi5Sultan Alshehri6Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Ad Diriyah 13713, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Ad Diriyah 13713, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaKing Abdullah International Medical Research Center, College of Pharmacy, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 11481, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, AlMaarefa University, Ad Diriyah 13713, Saudi ArabiaDespite the fact that several analytical methodologies have been reported for the determination of curcumin (CCM) in a wide range of sample matrices, the greener liquid chromatographic approaches to determine CCM are scarce in the literature. Therefore, this research is designed to develop and validate a greener stability-indicating “high-performance liquid chromatography (HPLC)” methodology to determine CCM in an in-house developed nanoemulsion, <i>Curcuma longa</i> L. extract, and commercial tablets. CCM was measured on a Nucleodur (150 mm × 4.6 mm) RP C<sub>18</sub> column with 5 µm-sized particles. Ethanol and ethyl acetate (83:17 <i>v/v</i>) made up the greener eluent system, which was pumped at a flow speed of 1.0 mL/min. At a wavelength of 425 nm, CCM was detected. The greener HPLC methodology was linear in the 1–100 µg/mL range, with a determination coefficient of 0.9983. The greener HPLC methodology for CCM estimation was also rapid (R<sub>t</sub> = 3.57 min), accurate (%recoveries = 98.90–101.85), precise (%CV = 0.90–1.11), and sensitive (LOD = 0.39 µg/mL and LOQ = 1.17 µg/mL). The AGREE approach predicted the AGREE score of 0.81 for the established HPLC technique, indicating an outstanding greenness profile. The utility of the greener HPLC methodology was demonstrated by determining CCM in the in-house developed nanoemulsion, <i>Curcuma longa</i> extract, and commercial tablets. The % amount of CCM in the in-house developed nanoemulsion, <i>Curcuma longa</i> extract, and commercial tablets was found to be 101.24%, 81.15%, and 78.41%, respectively. The greener HPLC methodology was able to detect its degradation product under various stress conditions, suggesting its stability-indication characteristics. These results suggested that CCM in developed nanoemulsion, plant extract samples, and commercial tablets may be routinely determined using the greener HPLC methodology.https://www.mdpi.com/2297-8739/10/2/98AGREE<i>Curcuma longa</i>curcuminnanoemulsiongreener HPLCvalidation |
| spellingShingle | Nazrul Haq Faiyaz Shakeel Mohammed M. Ghoneim Syed Mohammed Basheeruddin Asdaq Prawez Alam Saleh A. Alanazi Sultan Alshehri Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. Extract Separations AGREE <i>Curcuma longa</i> curcumin nanoemulsion greener HPLC validation |
| title | Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. Extract |
| title_full | Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. Extract |
| title_fullStr | Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. Extract |
| title_full_unstemmed | Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. Extract |
| title_short | Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and <i>Curcuma longa</i> L. Extract |
| title_sort | greener stability indicating hplc approach for the determination of curcumin in in house developed nanoemulsion and i curcuma longa i l extract |
| topic | AGREE <i>Curcuma longa</i> curcumin nanoemulsion greener HPLC validation |
| url | https://www.mdpi.com/2297-8739/10/2/98 |
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