Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle
This study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were u...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/6/370 |
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| author | Mona Al-Sharif Mohamed Abdo Omnia El Shabrawy Eman M. Abu El-Naga Liana Fericean Ioan Banatean-Dunea Ahmed Ateya |
| author_facet | Mona Al-Sharif Mohamed Abdo Omnia El Shabrawy Eman M. Abu El-Naga Liana Fericean Ioan Banatean-Dunea Ahmed Ateya |
| author_sort | Mona Al-Sharif |
| collection | DOAJ |
| description | This study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were used. Nucleotide sequence variations between healthy and endometritis-affected cows were revealed using PCR-DNA sequencing for immune (<i>TLR4</i>, <i>TLR7</i>, <i>TNF-α</i>, <i>IL10</i>, <i>NCF4</i>, and <i>LITAF</i>), antioxidant (<i>ATOX1</i>, <i>GST</i>, and <i>OXSR1</i>), and erythritol-related (<i>TKT</i>, <i>RPIA</i>, and <i>AMPD1</i>) genes. Chi-square investigation exposed a noteworthy variance amongst cow groups with and without endometritis in likelihood of dispersal of all distinguished nucleotide variants (<i>p</i> < 0.05). The <i>IL10</i>, <i>ATOX1</i>, and <i>GST</i> genes were expressed at substantially lower levels in endometritis-affected cows. Gene expression levels were considerably higher in endometritis-affected cows than in resistant ones for the genes <i>TLR4</i>, <i>TLR7</i>, <i>TNF-α</i>, <i>NCF4</i>, <i>LITAF</i>, <i>OXSR1</i>, <i>TKT</i>, <i>RPIA</i>, and <i>AMPD1</i>. The sort of marker and vulnerability or resistance to endometritis had a significant impact on the transcript levels of the studied indicators. The outcomes might confirm the importance of nucleotide variants along with gene expression patterns as markers of postparturient endometritis susceptibility/resistance and provide a workable control plan for Holstein dairy cows. |
| first_indexed | 2024-03-11T01:50:40Z |
| format | Article |
| id | doaj.art-0c67e0774802407296a4cace0e0b6afb |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T01:50:40Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj.art-0c67e0774802407296a4cace0e0b6afb2023-11-18T12:59:57ZengMDPI AGVeterinary Sciences2306-73812023-05-0110637010.3390/vetsci10060370Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein CattleMona Al-Sharif0Mohamed Abdo1Omnia El Shabrawy2Eman M. Abu El-Naga3Liana Fericean4Ioan Banatean-Dunea5Ahmed Ateya6Department of Biology, College of Science, University of Jeddah, Jeddah 21589, Saudi ArabiaDepartment of Animal Histology and Anatomy, School of Veterinary Medicine, Badr University in Cairo (BUC), Cairo 11829, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Menofia University, Menofia 32951, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, EgyptDepartment of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, RomaniaDepartment of Biology and Plant Protection, Faculty of Agricultural Sciences, University of Life Sciences King Michael I, 300645 Timisoara, RomaniaDepartment of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptThis study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were used. Nucleotide sequence variations between healthy and endometritis-affected cows were revealed using PCR-DNA sequencing for immune (<i>TLR4</i>, <i>TLR7</i>, <i>TNF-α</i>, <i>IL10</i>, <i>NCF4</i>, and <i>LITAF</i>), antioxidant (<i>ATOX1</i>, <i>GST</i>, and <i>OXSR1</i>), and erythritol-related (<i>TKT</i>, <i>RPIA</i>, and <i>AMPD1</i>) genes. Chi-square investigation exposed a noteworthy variance amongst cow groups with and without endometritis in likelihood of dispersal of all distinguished nucleotide variants (<i>p</i> < 0.05). The <i>IL10</i>, <i>ATOX1</i>, and <i>GST</i> genes were expressed at substantially lower levels in endometritis-affected cows. Gene expression levels were considerably higher in endometritis-affected cows than in resistant ones for the genes <i>TLR4</i>, <i>TLR7</i>, <i>TNF-α</i>, <i>NCF4</i>, <i>LITAF</i>, <i>OXSR1</i>, <i>TKT</i>, <i>RPIA</i>, and <i>AMPD1</i>. The sort of marker and vulnerability or resistance to endometritis had a significant impact on the transcript levels of the studied indicators. The outcomes might confirm the importance of nucleotide variants along with gene expression patterns as markers of postparturient endometritis susceptibility/resistance and provide a workable control plan for Holstein dairy cows.https://www.mdpi.com/2306-7381/10/6/370Holstein cattlegenetic polymorphismstranscript levelpostparturient endometritis |
| spellingShingle | Mona Al-Sharif Mohamed Abdo Omnia El Shabrawy Eman M. Abu El-Naga Liana Fericean Ioan Banatean-Dunea Ahmed Ateya Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle Veterinary Sciences Holstein cattle genetic polymorphisms transcript level postparturient endometritis |
| title | Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle |
| title_full | Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle |
| title_fullStr | Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle |
| title_full_unstemmed | Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle |
| title_short | Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle |
| title_sort | investigating polymorphisms and expression profile of immune antioxidant and erythritol related genes for limiting postparturient endometritis in holstein cattle |
| topic | Holstein cattle genetic polymorphisms transcript level postparturient endometritis |
| url | https://www.mdpi.com/2306-7381/10/6/370 |
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