NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON
The aim of the research is to increase the survivability of reproductive cells of sturgeon at cryopreservation and developing reliable technology suitable for use on an industrial scale.Methods. We have used standard methods of freezing, thawing reproductive cells, fertilization and incubation of eg...
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| Format: | Article |
| Language: | Russian |
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Kamerton
2016-03-01
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| Series: | Юг России: экология, развитие |
| Subjects: | |
| Online Access: | https://ecodag.elpub.ru/ugro/article/view/804 |
| _version_ | 1797880639666520064 |
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| author | E. N. Ponomareva A. A. Krasilnikova A. M. Tikhomirov A. V. Firsova |
| author_facet | E. N. Ponomareva A. A. Krasilnikova A. M. Tikhomirov A. V. Firsova |
| author_sort | E. N. Ponomareva |
| collection | DOAJ |
| description | The aim of the research is to increase the survivability of reproductive cells of sturgeon at cryopreservation and developing reliable technology suitable for use on an industrial scale.Methods. We have used standard methods of freezing, thawing reproductive cells, fertilization and incubation of eggs and larval rearing of sturgeon. Fundamentally new is cryoprotective composition: for sperm we have adjusted the composition of cryoprotective medium (for beluga 3% of dimethyl sulfoxide, for Russian sturgeon 4% of dimethyl sulfoxide); for freezing the eggs we have used cryoprotective mixture of unrefined vegetable and animal oils.Results. Survivability of defrosted sperm sturgeon has been increased: for Beluga it is up to 20%, for Russian sturgeon - 47%. At insemination of cryopreserved eggs of Russian sturgeon with native sperm the fertilization rate has made 41%.Main conclusions. The research proves the effectiveness of reducing the toxic effect of cryoprotective substances, thus leading to increased survivability of reproductive cells of sturgeon. During the insemination of eggs, stored in liquid nitrogen, the resulting offspring were viable and by the reactivity of the central nervous system and receptor complex it does not differ from the young obtained by conventional technology. |
| first_indexed | 2024-04-10T03:06:25Z |
| format | Article |
| id | doaj.art-0c689c377a384a98a0ca2546338ec681 |
| institution | Directory Open Access Journal |
| issn | 1992-1098 2413-0958 |
| language | Russian |
| last_indexed | 2024-04-10T03:06:25Z |
| publishDate | 2016-03-01 |
| publisher | Kamerton |
| record_format | Article |
| series | Юг России: экология, развитие |
| spelling | doaj.art-0c689c377a384a98a0ca2546338ec6812023-03-13T07:35:59ZrusKamertonЮг России: экология, развитие1992-10982413-09582016-03-01111596810.18470/1992-1098-2016-1-59-68804NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEONE. N. Ponomareva0A. A. Krasilnikova1A. M. Tikhomirov2A. V. Firsova3Южный научный центр Российской академии наукЮжный научный центр Российской академии наукАстраханский государственный технический университетЮжный научный центр Российской академии наукThe aim of the research is to increase the survivability of reproductive cells of sturgeon at cryopreservation and developing reliable technology suitable for use on an industrial scale.Methods. We have used standard methods of freezing, thawing reproductive cells, fertilization and incubation of eggs and larval rearing of sturgeon. Fundamentally new is cryoprotective composition: for sperm we have adjusted the composition of cryoprotective medium (for beluga 3% of dimethyl sulfoxide, for Russian sturgeon 4% of dimethyl sulfoxide); for freezing the eggs we have used cryoprotective mixture of unrefined vegetable and animal oils.Results. Survivability of defrosted sperm sturgeon has been increased: for Beluga it is up to 20%, for Russian sturgeon - 47%. At insemination of cryopreserved eggs of Russian sturgeon with native sperm the fertilization rate has made 41%.Main conclusions. The research proves the effectiveness of reducing the toxic effect of cryoprotective substances, thus leading to increased survivability of reproductive cells of sturgeon. During the insemination of eggs, stored in liquid nitrogen, the resulting offspring were viable and by the reactivity of the central nervous system and receptor complex it does not differ from the young obtained by conventional technology.https://ecodag.elpub.ru/ugro/article/view/804криоконсервациясперма рыбяйцеклеткикриопротекторосетровые рыбывыживаемостьсохранение генофондаискусственное воспроизводство |
| spellingShingle | E. N. Ponomareva A. A. Krasilnikova A. M. Tikhomirov A. V. Firsova NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON Юг России: экология, развитие криоконсервация сперма рыб яйцеклетки криопротектор осетровые рыбы выживаемость сохранение генофонда искусственное воспроизводство |
| title | NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON |
| title_full | NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON |
| title_fullStr | NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON |
| title_full_unstemmed | NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON |
| title_short | NEW BIOTECHNOLOGICAL METHODS FOR CRYOPRESERVATION OF REPRODUCTIVE CELLS OF STURGEON |
| title_sort | new biotechnological methods for cryopreservation of reproductive cells of sturgeon |
| topic | криоконсервация сперма рыб яйцеклетки криопротектор осетровые рыбы выживаемость сохранение генофонда искусственное воспроизводство |
| url | https://ecodag.elpub.ru/ugro/article/view/804 |
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