Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes
Abstract Background Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome (WNS), has led to the deaths of millions of hibernating bats in the United States of America (USA) and Canada. Efficient strategies are needed to decontaminate Pd from the bat hibernacula to inte...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Microbiome |
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| Online Access: | http://link.springer.com/article/10.1186/s40168-018-0512-6 |
| _version_ | 1818320531267518464 |
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| author | Amanpreet Singh Erica Lasek-Nesselquist Vishnu Chaturvedi Sudha Chaturvedi |
| author_facet | Amanpreet Singh Erica Lasek-Nesselquist Vishnu Chaturvedi Sudha Chaturvedi |
| author_sort | Amanpreet Singh |
| collection | DOAJ |
| description | Abstract Background Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome (WNS), has led to the deaths of millions of hibernating bats in the United States of America (USA) and Canada. Efficient strategies are needed to decontaminate Pd from the bat hibernacula to interrupt the disease transmission cycle without affecting the native microbes. Previously, we discovered a novel Trichoderma polysporum (Tp) strain (WPM 39143), which inhibited the growth of Pd in autoclaved soil samples. In the present investigation, we used culture-based approaches to determine Tp-induced killing of native and enriched Pd in the natural soil of two bat hibernacula. We also assessed the impact of Tp treatment on native microbial communities by metagenomics. Results Our results demonstrated that Tp at the concentration of 105 conidia/g soil caused 100% killing of native Pd in culture within 5 weeks of incubation. A 10-fold higher concentration of Tp (106 conidia/g soil) killed an enriched Pd population (105 conidia/g soil). The 12,507 fungal operational taxonomic units (OTUs, dominated by Ascomycota and Basidiomycota) and 27,427 bacterial OTUs (dominated by Acidobacteria and Proteobacteria) comprised the native soil microbes of the two bat hibernacula. No significant differences in fungal and bacterial relative abundances were observed between untreated and Tp-treated soil (105 Tp conidia/g soil, p ≤ 0.05). Conclusions Our results suggest that Tp-induced killing of Pd is highly specific, with minimal to no impact on the indigenous microbes present in the soil samples. These findings provide the scientific rationale for the field trials of Tp in the WNS-affected hibernacula for the effective decontamination of Pd and the control of WNS. |
| first_indexed | 2024-12-13T10:26:29Z |
| format | Article |
| id | doaj.art-0c69e36d48524a758c900c1e66dc0d7e |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-12-13T10:26:29Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-0c69e36d48524a758c900c1e66dc0d7e2022-12-21T23:51:00ZengBMCMicrobiome2049-26182018-08-016111110.1186/s40168-018-0512-6Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbesAmanpreet Singh0Erica Lasek-Nesselquist1Vishnu Chaturvedi2Sudha Chaturvedi3Mycology Laboratory, Wadsworth Center, New York State Department of HealthBioinformatics Core, Wadsworth Center, New York State Department of HealthMycology Laboratory, Wadsworth Center, New York State Department of HealthMycology Laboratory, Wadsworth Center, New York State Department of HealthAbstract Background Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome (WNS), has led to the deaths of millions of hibernating bats in the United States of America (USA) and Canada. Efficient strategies are needed to decontaminate Pd from the bat hibernacula to interrupt the disease transmission cycle without affecting the native microbes. Previously, we discovered a novel Trichoderma polysporum (Tp) strain (WPM 39143), which inhibited the growth of Pd in autoclaved soil samples. In the present investigation, we used culture-based approaches to determine Tp-induced killing of native and enriched Pd in the natural soil of two bat hibernacula. We also assessed the impact of Tp treatment on native microbial communities by metagenomics. Results Our results demonstrated that Tp at the concentration of 105 conidia/g soil caused 100% killing of native Pd in culture within 5 weeks of incubation. A 10-fold higher concentration of Tp (106 conidia/g soil) killed an enriched Pd population (105 conidia/g soil). The 12,507 fungal operational taxonomic units (OTUs, dominated by Ascomycota and Basidiomycota) and 27,427 bacterial OTUs (dominated by Acidobacteria and Proteobacteria) comprised the native soil microbes of the two bat hibernacula. No significant differences in fungal and bacterial relative abundances were observed between untreated and Tp-treated soil (105 Tp conidia/g soil, p ≤ 0.05). Conclusions Our results suggest that Tp-induced killing of Pd is highly specific, with minimal to no impact on the indigenous microbes present in the soil samples. These findings provide the scientific rationale for the field trials of Tp in the WNS-affected hibernacula for the effective decontamination of Pd and the control of WNS.http://link.springer.com/article/10.1186/s40168-018-0512-6White-nose syndromePseudogymnoascus destructansTrichoderma polysporumBiological decontaminationBiocontrol agentSelective inhibition |
| spellingShingle | Amanpreet Singh Erica Lasek-Nesselquist Vishnu Chaturvedi Sudha Chaturvedi Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes Microbiome White-nose syndrome Pseudogymnoascus destructans Trichoderma polysporum Biological decontamination Biocontrol agent Selective inhibition |
| title | Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes |
| title_full | Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes |
| title_fullStr | Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes |
| title_full_unstemmed | Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes |
| title_short | Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes |
| title_sort | trichoderma polysporum selectively inhibits white nose syndrome fungal pathogen pseudogymnoascus destructans amidst soil microbes |
| topic | White-nose syndrome Pseudogymnoascus destructans Trichoderma polysporum Biological decontamination Biocontrol agent Selective inhibition |
| url | http://link.springer.com/article/10.1186/s40168-018-0512-6 |
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