Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in Water
In this study, a new colistin-functionalized silica gel material (SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST) was synthesized after carboxylation on the surface of amino-modified silica. The main factors affecting the adsorptive properties of the material, such as the types of l...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/23/8292 |
| _version_ | 1797462628153425920 |
|---|---|
| author | Jingli Qiu Jianli Li Xiaoxi Du Tong Zhou Bingbing Xie Limin He |
| author_facet | Jingli Qiu Jianli Li Xiaoxi Du Tong Zhou Bingbing Xie Limin He |
| author_sort | Jingli Qiu |
| collection | DOAJ |
| description | In this study, a new colistin-functionalized silica gel material (SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST) was synthesized after carboxylation on the surface of amino-modified silica. The main factors affecting the adsorptive properties of the material, such as the types of linkers, the linking methods, the reaction buffers and the particle sizes of carriers, were systematically investigated. The SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST was characterized by means of electron microscopy, Fourier-transform infrared spectroscopy, zeta potential measurements, etc. We demonstrated that the sorbent showed good adsorption of Gram-negative bacteria. The adsorption efficiency of <i>E</i>. <i>coli</i> on SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST was 5.2 × 10<sup>11</sup> CFU/g, which was 3.5 times higher than that on SiO<sub>2</sub>@NH<sub>2</sub>@COOH, suggesting that electrostatic interactions between SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST and <i>E</i>. <i>coli</i> played a key role. The adsorption was quick, and was reached in 5 min. Both pseudo-first-order and pseudo-second-order kinetic models fit well with the dynamic adsorption process of SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST, indicating that physical adsorption and chemisorption might occur simultaneously during the adsorption process. SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST was successfully applied for the rapid capture of bacteria from water. The synthesized material could be used as a potential means of bacterial isolation and detection. |
| first_indexed | 2024-03-09T17:39:19Z |
| format | Article |
| id | doaj.art-0c6ac4ca70a246f5b112c98697d80694 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:39:19Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0c6ac4ca70a246f5b112c98697d806942023-11-24T11:39:50ZengMDPI AGMolecules1420-30492022-11-012723829210.3390/molecules27238292Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in WaterJingli Qiu0Jianli Li1Xiaoxi Du2Tong Zhou3Bingbing Xie4Limin He5Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaNational Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, ChinaIn this study, a new colistin-functionalized silica gel material (SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST) was synthesized after carboxylation on the surface of amino-modified silica. The main factors affecting the adsorptive properties of the material, such as the types of linkers, the linking methods, the reaction buffers and the particle sizes of carriers, were systematically investigated. The SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST was characterized by means of electron microscopy, Fourier-transform infrared spectroscopy, zeta potential measurements, etc. We demonstrated that the sorbent showed good adsorption of Gram-negative bacteria. The adsorption efficiency of <i>E</i>. <i>coli</i> on SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST was 5.2 × 10<sup>11</sup> CFU/g, which was 3.5 times higher than that on SiO<sub>2</sub>@NH<sub>2</sub>@COOH, suggesting that electrostatic interactions between SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST and <i>E</i>. <i>coli</i> played a key role. The adsorption was quick, and was reached in 5 min. Both pseudo-first-order and pseudo-second-order kinetic models fit well with the dynamic adsorption process of SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST, indicating that physical adsorption and chemisorption might occur simultaneously during the adsorption process. SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST was successfully applied for the rapid capture of bacteria from water. The synthesized material could be used as a potential means of bacterial isolation and detection.https://www.mdpi.com/1420-3049/27/23/8292colistin modifierSiO<sub>2</sub>@NH<sub>2</sub>@COOH@CSTcharacterizationrapid capturebacteriawater |
| spellingShingle | Jingli Qiu Jianli Li Xiaoxi Du Tong Zhou Bingbing Xie Limin He Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in Water Molecules colistin modifier SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST characterization rapid capture bacteria water |
| title | Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in Water |
| title_full | Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in Water |
| title_fullStr | Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in Water |
| title_full_unstemmed | Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in Water |
| title_short | Synthesis and Characterization of Colistin-Functionalized Silica Materials for Rapid Capture of Bacteria in Water |
| title_sort | synthesis and characterization of colistin functionalized silica materials for rapid capture of bacteria in water |
| topic | colistin modifier SiO<sub>2</sub>@NH<sub>2</sub>@COOH@CST characterization rapid capture bacteria water |
| url | https://www.mdpi.com/1420-3049/27/23/8292 |
| work_keys_str_mv | AT jingliqiu synthesisandcharacterizationofcolistinfunctionalizedsilicamaterialsforrapidcaptureofbacteriainwater AT jianlili synthesisandcharacterizationofcolistinfunctionalizedsilicamaterialsforrapidcaptureofbacteriainwater AT xiaoxidu synthesisandcharacterizationofcolistinfunctionalizedsilicamaterialsforrapidcaptureofbacteriainwater AT tongzhou synthesisandcharacterizationofcolistinfunctionalizedsilicamaterialsforrapidcaptureofbacteriainwater AT bingbingxie synthesisandcharacterizationofcolistinfunctionalizedsilicamaterialsforrapidcaptureofbacteriainwater AT liminhe synthesisandcharacterizationofcolistinfunctionalizedsilicamaterialsforrapidcaptureofbacteriainwater |