Assaying <i>Paenibacillus alvei</i> CsaB-Catalysed Ketalpyruvyltransfer to Saccharides by Measurement of Phosphate Release

Assaying <i>Paenibacillus alvei</i> CsaB-Catalysed Ketalpyruvyltransfer to Saccharides by Measurement of Phosphate Release

Ketalpyruvyltransferases belong to a widespread but little investigated class of enzymes, which utilise phosphoenolpyruvate (PEP) for the pyruvylation of saccharides. Pyruvylated saccharides play pivotal biological roles, ranging from protein binding to virulence. Limiting factors for the characteri...

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Bibliographic Details
Main Authors: Fiona F. Hager-Mair, Cordula Stefanović, Charlie Lim, Katharina Webhofer, Simon Krauter, Markus Blaukopf, Roland Ludwig, Paul Kosma, Christina Schäffer
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:Biomolecules
Subjects:
cell wall glycopolymer
enzyme assay
kinetic constants
pyruvyltransferase
substrate synthesis
Online Access:https://www.mdpi.com/2218-273X/11/11/1732

Internet

https://www.mdpi.com/2218-273X/11/11/1732

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