Intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photons
Abstract In this work, an intercomparison of sensitization effects produced by gold (GNP) and dextran-coated iron oxide (SPION-DX) nanoparticles in M059J and U87 human glioblastoma cells was performed using 6 MV-photons. Three variables were mapped: the nanoparticle material, treatment concentration...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13368-x |
| _version_ | 1818207579221786624 |
|---|---|
| author | Danieli B. Guerra Elisa M. N. Oliveira Amanda R. Sonntag Patricia Sbaraine Andre P. Fay Fernanda B. Morrone Ricardo M. Papaléo |
| author_facet | Danieli B. Guerra Elisa M. N. Oliveira Amanda R. Sonntag Patricia Sbaraine Andre P. Fay Fernanda B. Morrone Ricardo M. Papaléo |
| author_sort | Danieli B. Guerra |
| collection | DOAJ |
| description | Abstract In this work, an intercomparison of sensitization effects produced by gold (GNP) and dextran-coated iron oxide (SPION-DX) nanoparticles in M059J and U87 human glioblastoma cells was performed using 6 MV-photons. Three variables were mapped: the nanoparticle material, treatment concentration, and cell radiosensitivity. For U87, GNP treatments resulted in high sensitization enhancement ratios (SER $$_{10\%}$$ 10 % up to 2.04). More modest effects were induced by SPION-DX, but still significant reductions in survival were achieved (maximum SER $$_{10\%}=1.61$$ 10 % = 1.61 ). For the radiosensitive M059J, sensitization by both NPs was poor. SER $$_{10\%}$$ 10 % increased with the degree of elemental uptake in the cells, but not necessarily with treatment concentration. For GNP, where exposure concentration and elemental uptake were found to be proportional, SER $$_{10\%}$$ 10 % increased linearly with concentration in both cell lines. For SPION-DX, saturation of sensitization enhancement and metal uptake occurred at high exposures. Fold change in the $$\alpha /\beta$$ α / β ratios extracted from survival curves are reduced by the presence of SPION-DX but strongly increased by GNPs , suggesting that sensitization by GNPs occurs mainly via promotion of lethal damage, while for SPION-DX repairable damage dominates. The NPs were more effective in eliminating the radioresistant glioblastoma cells, an interesting finding, as resistant cells are key targets to improve treatment outcome. |
| first_indexed | 2024-12-12T04:31:10Z |
| format | Article |
| id | doaj.art-0c6cc2841f3348fd881508c1d996a4c4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T04:31:10Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0c6cc2841f3348fd881508c1d996a4c42022-12-22T00:38:05ZengNature PortfolioScientific Reports2045-23222022-06-0112111110.1038/s41598-022-13368-xIntercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photonsDanieli B. Guerra0Elisa M. N. Oliveira1Amanda R. Sonntag2Patricia Sbaraine3Andre P. Fay4Fernanda B. Morrone5Ricardo M. Papaléo6Interdisciplinary Center of Nanoscience and Micro-Nanotechnology, School of Technology, Pontifical Catholic University of Rio Grande do SulInterdisciplinary Center of Nanoscience and Micro-Nanotechnology, School of Technology, Pontifical Catholic University of Rio Grande do SulInterdisciplinary Center of Nanoscience and Micro-Nanotechnology, School of Technology, Pontifical Catholic University of Rio Grande do SulDivision of Radiotherapy, São Lucas Hospital of PUCRSSchool of Medicine, Pontifical Catholic University of Rio Grande do SulSchool of Health and Life Sciences, Pontifical Catholic University of Rio Grande do SulInterdisciplinary Center of Nanoscience and Micro-Nanotechnology, School of Technology, Pontifical Catholic University of Rio Grande do SulAbstract In this work, an intercomparison of sensitization effects produced by gold (GNP) and dextran-coated iron oxide (SPION-DX) nanoparticles in M059J and U87 human glioblastoma cells was performed using 6 MV-photons. Three variables were mapped: the nanoparticle material, treatment concentration, and cell radiosensitivity. For U87, GNP treatments resulted in high sensitization enhancement ratios (SER $$_{10\%}$$ 10 % up to 2.04). More modest effects were induced by SPION-DX, but still significant reductions in survival were achieved (maximum SER $$_{10\%}=1.61$$ 10 % = 1.61 ). For the radiosensitive M059J, sensitization by both NPs was poor. SER $$_{10\%}$$ 10 % increased with the degree of elemental uptake in the cells, but not necessarily with treatment concentration. For GNP, where exposure concentration and elemental uptake were found to be proportional, SER $$_{10\%}$$ 10 % increased linearly with concentration in both cell lines. For SPION-DX, saturation of sensitization enhancement and metal uptake occurred at high exposures. Fold change in the $$\alpha /\beta$$ α / β ratios extracted from survival curves are reduced by the presence of SPION-DX but strongly increased by GNPs , suggesting that sensitization by GNPs occurs mainly via promotion of lethal damage, while for SPION-DX repairable damage dominates. The NPs were more effective in eliminating the radioresistant glioblastoma cells, an interesting finding, as resistant cells are key targets to improve treatment outcome.https://doi.org/10.1038/s41598-022-13368-x |
| spellingShingle | Danieli B. Guerra Elisa M. N. Oliveira Amanda R. Sonntag Patricia Sbaraine Andre P. Fay Fernanda B. Morrone Ricardo M. Papaléo Intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photons Scientific Reports |
| title | Intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photons |
| title_full | Intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photons |
| title_fullStr | Intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photons |
| title_full_unstemmed | Intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photons |
| title_short | Intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 MV photons |
| title_sort | intercomparison of radiosensitization induced by gold and iron oxide nanoparticles in human glioblastoma cells irradiated by 6 mv photons |
| url | https://doi.org/10.1038/s41598-022-13368-x |
| work_keys_str_mv | AT danielibguerra intercomparisonofradiosensitizationinducedbygoldandironoxidenanoparticlesinhumanglioblastomacellsirradiatedby6mvphotons AT elisamnoliveira intercomparisonofradiosensitizationinducedbygoldandironoxidenanoparticlesinhumanglioblastomacellsirradiatedby6mvphotons AT amandarsonntag intercomparisonofradiosensitizationinducedbygoldandironoxidenanoparticlesinhumanglioblastomacellsirradiatedby6mvphotons AT patriciasbaraine intercomparisonofradiosensitizationinducedbygoldandironoxidenanoparticlesinhumanglioblastomacellsirradiatedby6mvphotons AT andrepfay intercomparisonofradiosensitizationinducedbygoldandironoxidenanoparticlesinhumanglioblastomacellsirradiatedby6mvphotons AT fernandabmorrone intercomparisonofradiosensitizationinducedbygoldandironoxidenanoparticlesinhumanglioblastomacellsirradiatedby6mvphotons AT ricardompapaleo intercomparisonofradiosensitizationinducedbygoldandironoxidenanoparticlesinhumanglioblastomacellsirradiatedby6mvphotons |