Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis
Purpose: A rapid and efficient diagnostic test was developed for the detection of Mycobacterium tuberculosis antigens in serum samples of active tuberculosis (TB) and extrapulmonary TB patients via a liposomal agglutination-based method. Methods: A rapid card test has been developed to facilitate th...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118215007690 |
| _version_ | 1819058047438290944 |
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| author | Dileep Tiwari Shafiul Haque Ram P. Tiwari Arshad Jawed Thavendran Govender Hendrik G. Kruger |
| author_facet | Dileep Tiwari Shafiul Haque Ram P. Tiwari Arshad Jawed Thavendran Govender Hendrik G. Kruger |
| author_sort | Dileep Tiwari |
| collection | DOAJ |
| description | Purpose: A rapid and efficient diagnostic test was developed for the detection of Mycobacterium tuberculosis antigens in serum samples of active tuberculosis (TB) and extrapulmonary TB patients via a liposomal agglutination-based method.
Methods: A rapid card test has been developed to facilitate the recognition of high-affinity binding rabbit raised purified culture filtrate protein antibodies coupled on the surface of activated liposomal preparation. In the presence of TB antigens, the polyclonal antibodies bound to the liposomal particles demonstrate a visible agglutination reaction.
Results: The developed assay was simple, rapid, reliable, sensitive, and specific as a diagnostic test for the detection of antigens in serum samples of clinically confirmed cases of TB within 4–5 minutes' duration. The test was evaluated at different hospitals, medical colleges, and pathology centers, and involved 1483 participants. This investigation was conducted to detect the presence of these antigens during the period of active growth of the microorganism in serum samples for pulmonary TB and processed tissue biopsy for other extrapulmonary TB. Results obtained using this test were compared with acid-fast bacilli smear and culture results.
Conclusion: Our study demonstrated that the newly developed liposome tuberculosis antigen card test detected antigens in our study population with approximately 97.48% sensitivity and 95.79% specificity. This is the first study to report the liposomal encapsulation of culture filtrate proteins from M. tuberculosis for diagnostic application. |
| first_indexed | 2024-12-21T13:48:59Z |
| format | Article |
| id | doaj.art-0c6e6ba03966406f89ef6e9ef0dd4c6d |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-21T13:48:59Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-0c6e6ba03966406f89ef6e9ef0dd4c6d2022-12-21T19:01:47ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822017-04-0150218919810.1016/j.jmii.2015.05.014Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosisDileep Tiwari0Shafiul Haque1Ram P. Tiwari2Arshad Jawed3Thavendran Govender4Hendrik G. Kruger5Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South AfricaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Biotechnology, Immunodiagnostic Division, Vanguard Diagnostic Pvt. Ltd., Delhi 110020, IndiaResearch and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan 45142, Saudi ArabiaCatalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South AfricaCatalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South AfricaPurpose: A rapid and efficient diagnostic test was developed for the detection of Mycobacterium tuberculosis antigens in serum samples of active tuberculosis (TB) and extrapulmonary TB patients via a liposomal agglutination-based method. Methods: A rapid card test has been developed to facilitate the recognition of high-affinity binding rabbit raised purified culture filtrate protein antibodies coupled on the surface of activated liposomal preparation. In the presence of TB antigens, the polyclonal antibodies bound to the liposomal particles demonstrate a visible agglutination reaction. Results: The developed assay was simple, rapid, reliable, sensitive, and specific as a diagnostic test for the detection of antigens in serum samples of clinically confirmed cases of TB within 4–5 minutes' duration. The test was evaluated at different hospitals, medical colleges, and pathology centers, and involved 1483 participants. This investigation was conducted to detect the presence of these antigens during the period of active growth of the microorganism in serum samples for pulmonary TB and processed tissue biopsy for other extrapulmonary TB. Results obtained using this test were compared with acid-fast bacilli smear and culture results. Conclusion: Our study demonstrated that the newly developed liposome tuberculosis antigen card test detected antigens in our study population with approximately 97.48% sensitivity and 95.79% specificity. This is the first study to report the liposomal encapsulation of culture filtrate proteins from M. tuberculosis for diagnostic application.http://www.sciencedirect.com/science/article/pii/S1684118215007690Active tuberculosisAntigenLiposomeMycobacterium tuberculosis H37RvSecretory protein antibody |
| spellingShingle | Dileep Tiwari Shafiul Haque Ram P. Tiwari Arshad Jawed Thavendran Govender Hendrik G. Kruger Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis Journal of Microbiology, Immunology and Infection Active tuberculosis Antigen Liposome Mycobacterium tuberculosis H37Rv Secretory protein antibody |
| title | Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis |
| title_full | Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis |
| title_fullStr | Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis |
| title_full_unstemmed | Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis |
| title_short | Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis |
| title_sort | fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of mycobacterium tuberculosis |
| topic | Active tuberculosis Antigen Liposome Mycobacterium tuberculosis H37Rv Secretory protein antibody |
| url | http://www.sciencedirect.com/science/article/pii/S1684118215007690 |
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