Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development
Abstract Background Root hydraulic conductance is primarily determined by the conductance of living tissues to radial water flow. Plasma membrane intrinsic proteins (PIPs) in root cortical cells are important for plants to take up water and are believed to be directly involved in cell growth. Result...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03017-2 |
| _version_ | 1818431440299229184 |
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| author | Huani Leng Cheng Jiang Xueqin Song Mengzhu Lu Xianchong Wan |
| author_facet | Huani Leng Cheng Jiang Xueqin Song Mengzhu Lu Xianchong Wan |
| author_sort | Huani Leng |
| collection | DOAJ |
| description | Abstract Background Root hydraulic conductance is primarily determined by the conductance of living tissues to radial water flow. Plasma membrane intrinsic proteins (PIPs) in root cortical cells are important for plants to take up water and are believed to be directly involved in cell growth. Results In this study, we found that constitutive overexpression of the poplar root-specific gene PtoPIP1;1 in Arabidopsis accelerated bolting and flowering. At the early stage of the developmental process, PtoPIP1;1 OE Arabidopsis exhibited faster cell growth in both leaves and roots. The turgor pressure of plants was correspondingly increased in PtoPIP1;1 OE Arabidopsis, and the water status was changed. At the same time, the expression levels of flowering-related genes (CRY1, CRY2 and FCA) and hub genes in the regulatory networks underlying floral timing (FT and SOC1) were significantly upregulated in OE plants, while the floral repressor FLC gene was significantly downregulated. Conclusions Taken together, the results of our study indicate that constitutive overexpression of PtoPIP1;1 in Arabidopsis accelerates bolting and flowering through faster cell growth in both the leaf and root at an early stage of the developmental process. The autonomous pathway of flowering regulation may be executed by monitoring developmental age. The increase in turgor and changes in water status with PtoPIP1;1 overexpression play a role in promoting cell growth. |
| first_indexed | 2024-12-14T15:49:20Z |
| format | Article |
| id | doaj.art-0c70e34a805e467e848cd06b6bf56294 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-14T15:49:20Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-0c70e34a805e467e848cd06b6bf562942022-12-21T22:55:24ZengBMCBMC Plant Biology1471-22292021-06-0121111210.1186/s12870-021-03017-2Poplar aquaporin PIP1;1 promotes Arabidopsis growth and developmentHuani Leng0Cheng Jiang1Xueqin Song2Mengzhu Lu3Xianchong Wan4Institute of New Forestry Technology, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of ForestryInstitute of New Forestry Technology, Chinese Academy of ForestryAbstract Background Root hydraulic conductance is primarily determined by the conductance of living tissues to radial water flow. Plasma membrane intrinsic proteins (PIPs) in root cortical cells are important for plants to take up water and are believed to be directly involved in cell growth. Results In this study, we found that constitutive overexpression of the poplar root-specific gene PtoPIP1;1 in Arabidopsis accelerated bolting and flowering. At the early stage of the developmental process, PtoPIP1;1 OE Arabidopsis exhibited faster cell growth in both leaves and roots. The turgor pressure of plants was correspondingly increased in PtoPIP1;1 OE Arabidopsis, and the water status was changed. At the same time, the expression levels of flowering-related genes (CRY1, CRY2 and FCA) and hub genes in the regulatory networks underlying floral timing (FT and SOC1) were significantly upregulated in OE plants, while the floral repressor FLC gene was significantly downregulated. Conclusions Taken together, the results of our study indicate that constitutive overexpression of PtoPIP1;1 in Arabidopsis accelerates bolting and flowering through faster cell growth in both the leaf and root at an early stage of the developmental process. The autonomous pathway of flowering regulation may be executed by monitoring developmental age. The increase in turgor and changes in water status with PtoPIP1;1 overexpression play a role in promoting cell growth.https://doi.org/10.1186/s12870-021-03017-2PtoPIP1;1Flowering timeAutonomous pathwayGrowthTurgor pressure |
| spellingShingle | Huani Leng Cheng Jiang Xueqin Song Mengzhu Lu Xianchong Wan Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development BMC Plant Biology PtoPIP1;1 Flowering time Autonomous pathway Growth Turgor pressure |
| title | Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development |
| title_full | Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development |
| title_fullStr | Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development |
| title_full_unstemmed | Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development |
| title_short | Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development |
| title_sort | poplar aquaporin pip1 1 promotes arabidopsis growth and development |
| topic | PtoPIP1;1 Flowering time Autonomous pathway Growth Turgor pressure |
| url | https://doi.org/10.1186/s12870-021-03017-2 |
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