Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i>
Yeast <i>S. cerevisiae</i> has been shown to suppress a sterol biosynthesis as a response to hyperosmotic stress. In the case of sodium stress, the failure to suppress biosynthesis leads to an increase in cytosolic sodium. The major yeast sterol, ergosterol, is known to regulate function...
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MDPI AG
2022-12-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/12/1278 |
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| author | Svyatoslav S. Sokolov Marina M. Popova Peter Pohl Andreas Horner Sergey A. Akimov Natalia A. Kireeva Dmitry A. Knorre Oleg V. Batishchev Fedor F. Severin |
| author_facet | Svyatoslav S. Sokolov Marina M. Popova Peter Pohl Andreas Horner Sergey A. Akimov Natalia A. Kireeva Dmitry A. Knorre Oleg V. Batishchev Fedor F. Severin |
| author_sort | Svyatoslav S. Sokolov |
| collection | DOAJ |
| description | Yeast <i>S. cerevisiae</i> has been shown to suppress a sterol biosynthesis as a response to hyperosmotic stress. In the case of sodium stress, the failure to suppress biosynthesis leads to an increase in cytosolic sodium. The major yeast sterol, ergosterol, is known to regulate functioning of plasma membrane proteins. Therefore, it has been suggested that the suppression of its biosynthesis is needed to adjust the activity of the plasma membrane sodium pumps and channels. However, as the sterol concentration is in the range of thirty to forty percent of total plasma membrane lipids, it is believed that its primary biological role is not regulatory but structural. Here we studied how lowering the sterol content affects the response of a lipid bilayer to an osmotic stress. In accordance with previous observations, we found that a decrease of the sterol fraction increases a water permeability of the liposomal membranes. Yet, we also found that sterol-free giant unilamellar vesicles reduced their volume during transient application of the hyperosmotic stress to a greater extent than the sterol-rich ones. Furthermore, our data suggest that lowering the sterol content in yeast cells allows the shrinkage to prevent the osmotic pressure-induced plasma membrane rupture. We also found that mutant yeast cells with the elevated level of sterol accumulated propidium iodide when exposed to mild hyperosmotic conditions followed by hypoosmotic stress. It is likely that the decrease in a plasma membrane sterol content stimulates a drop in cell volume under hyperosmotic stress, which is beneficial in the case of a subsequent hypo-osmotic one. |
| first_indexed | 2024-03-09T16:06:44Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T16:06:44Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-0c78cdd056db4d9fae5e6bc35eea38ec2023-11-24T16:36:30ZengMDPI AGMembranes2077-03752022-12-011212127810.3390/membranes12121278Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i>Svyatoslav S. Sokolov0Marina M. Popova1Peter Pohl2Andreas Horner3Sergey A. Akimov4Natalia A. Kireeva5Dmitry A. Knorre6Oleg V. Batishchev7Fedor F. Severin8Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskie Gory, 119991 Moscow, RussiaFrumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4 Leninskiyprospekt, 119071 Moscow, RussiaInstitute of Biophysics, Johannes Kepler University Linz, Gruberstraße 40, 4020 Linz, AustriaInstitute of Biophysics, Johannes Kepler University Linz, Gruberstraße 40, 4020 Linz, AustriaFrumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4 Leninskiyprospekt, 119071 Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskie Gory, 119991 Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskie Gory, 119991 Moscow, RussiaFrumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4 Leninskiyprospekt, 119071 Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 1-40 Leninskie Gory, 119991 Moscow, RussiaYeast <i>S. cerevisiae</i> has been shown to suppress a sterol biosynthesis as a response to hyperosmotic stress. In the case of sodium stress, the failure to suppress biosynthesis leads to an increase in cytosolic sodium. The major yeast sterol, ergosterol, is known to regulate functioning of plasma membrane proteins. Therefore, it has been suggested that the suppression of its biosynthesis is needed to adjust the activity of the plasma membrane sodium pumps and channels. However, as the sterol concentration is in the range of thirty to forty percent of total plasma membrane lipids, it is believed that its primary biological role is not regulatory but structural. Here we studied how lowering the sterol content affects the response of a lipid bilayer to an osmotic stress. In accordance with previous observations, we found that a decrease of the sterol fraction increases a water permeability of the liposomal membranes. Yet, we also found that sterol-free giant unilamellar vesicles reduced their volume during transient application of the hyperosmotic stress to a greater extent than the sterol-rich ones. Furthermore, our data suggest that lowering the sterol content in yeast cells allows the shrinkage to prevent the osmotic pressure-induced plasma membrane rupture. We also found that mutant yeast cells with the elevated level of sterol accumulated propidium iodide when exposed to mild hyperosmotic conditions followed by hypoosmotic stress. It is likely that the decrease in a plasma membrane sterol content stimulates a drop in cell volume under hyperosmotic stress, which is beneficial in the case of a subsequent hypo-osmotic one.https://www.mdpi.com/2077-0375/12/12/1278sterolhyperosmotic stresshypoosmotic stressyeastgiant unilamellar vesiclelarge unilamellar vesicle |
| spellingShingle | Svyatoslav S. Sokolov Marina M. Popova Peter Pohl Andreas Horner Sergey A. Akimov Natalia A. Kireeva Dmitry A. Knorre Oleg V. Batishchev Fedor F. Severin Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i> Membranes sterol hyperosmotic stress hypoosmotic stress yeast giant unilamellar vesicle large unilamellar vesicle |
| title | Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i> |
| title_full | Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i> |
| title_short | Structural Role of Plasma Membrane Sterols in Osmotic Stress Tolerance of Yeast <i>Saccharomyces cerevisiae</i> |
| title_sort | structural role of plasma membrane sterols in osmotic stress tolerance of yeast i saccharomyces cerevisiae i |
| topic | sterol hyperosmotic stress hypoosmotic stress yeast giant unilamellar vesicle large unilamellar vesicle |
| url | https://www.mdpi.com/2077-0375/12/12/1278 |
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