Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术)
以天目铁木嫩茎尖为外植体，应用均匀设计法筛选其基部愈伤组织诱导和愈伤组织再分化芽苗的最适合培养基.结果表明，最适合的嫩茎尖基部愈伤组织诱导培养基为1/2DR+TDZ 2.30 mg • L-1 +2，4-D 0.55 mg• L-1，诱导率为93.5%；愈伤组织芽苗再分化培养基为1/2DR+TDZ 3.30 mg • L-1+KT 0.70 mg • L-1，分化率达99.8%以上.成功建立了天目铁木嫩茎尖离体诱导愈伤组织和芽苗再分化体系，且再生芽苗与野生植株染色体数目相同....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2013-03-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2013.02.020 |
| _version_ | 1797235991677763584 |
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| author | GUDi-zhou(顾地周) LUShuang(陆爽) BAChun-ying(巴春影) LIYuan-yuan(李媛媛) |
| author_facet | GUDi-zhou(顾地周) LUShuang(陆爽) BAChun-ying(巴春影) LIYuan-yuan(李媛媛) |
| author_sort | GUDi-zhou(顾地周) |
| collection | DOAJ |
| description | 以天目铁木嫩茎尖为外植体，应用均匀设计法筛选其基部愈伤组织诱导和愈伤组织再分化芽苗的最适合培养基.结果表明，最适合的嫩茎尖基部愈伤组织诱导培养基为1/2DR+TDZ 2.30 mg • L-1 +2，4-D 0.55 mg• L-1，诱导率为93.5%；愈伤组织芽苗再分化培养基为1/2DR+TDZ 3.30 mg • L-1+KT 0.70 mg • L-1，分化率达99.8%以上.成功建立了天目铁木嫩茎尖离体诱导愈伤组织和芽苗再分化体系，且再生芽苗与野生植株染色体数目相同. |
| first_indexed | 2024-04-24T16:56:45Z |
| format | Article |
| id | doaj.art-0c7b4dc81906471bb66d1d0a8f98ddb4 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:56:45Z |
| publishDate | 2013-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-0c7b4dc81906471bb66d1d0a8f98ddb42024-03-29T01:58:31ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972013-03-0140221622010.3785/j.issn.1008-9497.2013.02.020Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术)GUDi-zhou(顾地周)0LUShuang(陆爽)1BAChun-ying(巴春影)2LIYuan-yuan(李媛媛)3Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin Province, China(通化师范学院 生物系，吉林 通化 134002)Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin Province, China(通化师范学院 生物系，吉林 通化 134002)Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin Province, China(通化师范学院 生物系，吉林 通化 134002)Department of Biology, Tonghua Normal University, Tonghua 134002, Jilin Province, China(通化师范学院 生物系，吉林 通化 134002)以天目铁木嫩茎尖为外植体，应用均匀设计法筛选其基部愈伤组织诱导和愈伤组织再分化芽苗的最适合培养基.结果表明，最适合的嫩茎尖基部愈伤组织诱导培养基为1/2DR+TDZ 2.30 mg • L-1 +2，4-D 0.55 mg• L-1，诱导率为93.5%；愈伤组织芽苗再分化培养基为1/2DR+TDZ 3.30 mg • L-1+KT 0.70 mg • L-1，分化率达99.8%以上.成功建立了天目铁木嫩茎尖离体诱导愈伤组织和芽苗再分化体系，且再生芽苗与野生植株染色体数目相同.https://doi.org/10.3785/j.issn.1008-9497.2013.02.020天目铁木愈伤组织诱导再分化均匀设计 |
| spellingShingle | GUDi-zhou(顾地周) LUShuang(陆爽) BAChun-ying(巴春影) LIYuan-yuan(李媛媛) Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术) Zhejiang Daxue xuebao. Lixue ban 天目铁木 愈伤组织 诱导 再分化 均匀设计 |
| title | Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术) |
| title_full | Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术) |
| title_fullStr | Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术) |
| title_full_unstemmed | Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术) |
| title_short | Technique of callus induction and bud seedling of Ostrya rehderiana Chun(天目铁木愈伤组织和芽苗诱导技术) |
| title_sort | technique of callus induction and bud seedling of ostrya rehderiana chun 天目铁木愈伤组织和芽苗诱导技术 |
| topic | 天目铁木 愈伤组织 诱导 再分化 均匀设计 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2013.02.020 |
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