Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i>
Many plants show significant biological activity against pests due to their unique chemical constituents. It is important to identify effective constituents for their development and utilization as botanical pesticides. Our previous study showed that <i>Artemisia lavandulaefolia</i> esse...
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MDPI AG
2021-11-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/12/842 |
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| author | Xing Huang Yulin Huang Chunyue Yang Tiantian Liu Xing Liu Haibin Yuan |
| author_facet | Xing Huang Yulin Huang Chunyue Yang Tiantian Liu Xing Liu Haibin Yuan |
| author_sort | Xing Huang |
| collection | DOAJ |
| description | Many plants show significant biological activity against pests due to their unique chemical constituents. It is important to identify effective constituents for their development and utilization as botanical pesticides. Our previous study showed that <i>Artemisia lavandulaefolia</i> essential oil had biological activity against <i>Plutella xylostella</i>. Here, we isolated and identified the constituents of essential oil from <i>A</i>. <i>lavandulaefolia</i> by silica gel column chromatography. The main constituents identified were eucalyptol and caryophyllene oxide, and they were confirmed by gas chromatography–mass spectrometry (GC–MS). Eucalyptol and caryophyllene oxide showed strong contact toxicity against <i>P. xylostella</i> larvae after 24 h of application (Median lethal dose, LD<sub>50</sub> = 76.97 μL/mL and 20.71 mg/mL. Furthermore, the two active constituents against <i>P. xylostella</i> adults showed significant fumigant activity (Mmedian lethal concentration, LC<sub>50</sub> = 3.25 μL/L and 1.06 mg/L, respectively. Finally, we measured the detoxification enzymes and acetylcholinesterase of the larvae treated with active constituents. The eucalyptol-treated larvae displayed enhanced carboxylesterase (CarE) and glutathione-S-transferase (GST) activities in an in vivo experiment, but it was lower for acetylcholinesterase (AchE) activity. The activities of the CarE and GST significantly decreased when exposed to caryophyllene oxide. In general, the two active constituents, eucalyptol and caryophyllene oxide, showed high insecticidal activity, which demonstrates their potential to be used as natural insecticides. |
| first_indexed | 2024-03-10T03:57:40Z |
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| language | English |
| last_indexed | 2024-03-10T03:57:40Z |
| publishDate | 2021-11-01 |
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| record_format | Article |
| series | Toxins |
| spelling | doaj.art-0c7b80fe4d364a60b61d284f25d7410b2023-11-23T10:50:18ZengMDPI AGToxins2072-66512021-11-01131284210.3390/toxins13120842Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i>Xing Huang0Yulin Huang1Chunyue Yang2Tiantian Liu3Xing Liu4Haibin Yuan5College of Plant Protection, Jilin Agricultural University, Changchun 130118, ChinaCollege of Plant Protection, Jilin Agricultural University, Changchun 130118, ChinaCollege of Plant Protection, Jilin Agricultural University, Changchun 130118, ChinaCollege of Plant Protection, Jilin Agricultural University, Changchun 130118, ChinaCollege of Plant Protection, Jilin Agricultural University, Changchun 130118, ChinaCollege of Plant Protection, Jilin Agricultural University, Changchun 130118, ChinaMany plants show significant biological activity against pests due to their unique chemical constituents. It is important to identify effective constituents for their development and utilization as botanical pesticides. Our previous study showed that <i>Artemisia lavandulaefolia</i> essential oil had biological activity against <i>Plutella xylostella</i>. Here, we isolated and identified the constituents of essential oil from <i>A</i>. <i>lavandulaefolia</i> by silica gel column chromatography. The main constituents identified were eucalyptol and caryophyllene oxide, and they were confirmed by gas chromatography–mass spectrometry (GC–MS). Eucalyptol and caryophyllene oxide showed strong contact toxicity against <i>P. xylostella</i> larvae after 24 h of application (Median lethal dose, LD<sub>50</sub> = 76.97 μL/mL and 20.71 mg/mL. Furthermore, the two active constituents against <i>P. xylostella</i> adults showed significant fumigant activity (Mmedian lethal concentration, LC<sub>50</sub> = 3.25 μL/L and 1.06 mg/L, respectively. Finally, we measured the detoxification enzymes and acetylcholinesterase of the larvae treated with active constituents. The eucalyptol-treated larvae displayed enhanced carboxylesterase (CarE) and glutathione-S-transferase (GST) activities in an in vivo experiment, but it was lower for acetylcholinesterase (AchE) activity. The activities of the CarE and GST significantly decreased when exposed to caryophyllene oxide. In general, the two active constituents, eucalyptol and caryophyllene oxide, showed high insecticidal activity, which demonstrates their potential to be used as natural insecticides.https://www.mdpi.com/2072-6651/13/12/842<i>Artemisia lavandulaefolia</i> DC<i>Plutella xylostella</i>fractionationinsecticidal activity |
| spellingShingle | Xing Huang Yulin Huang Chunyue Yang Tiantian Liu Xing Liu Haibin Yuan Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i> Toxins <i>Artemisia lavandulaefolia</i> DC <i>Plutella xylostella</i> fractionation insecticidal activity |
| title | Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i> |
| title_full | Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i> |
| title_fullStr | Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i> |
| title_full_unstemmed | Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i> |
| title_short | Isolation and Insecticidal Activity of Essential Oil from <i>Artemisia lavandulaefolia</i> DC. against <i>Plutella xylostella</i> |
| title_sort | isolation and insecticidal activity of essential oil from i artemisia lavandulaefolia i dc against i plutella xylostella i |
| topic | <i>Artemisia lavandulaefolia</i> DC <i>Plutella xylostella</i> fractionation insecticidal activity |
| url | https://www.mdpi.com/2072-6651/13/12/842 |
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