Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement Strategies
Selenium-enriched yeast possesses the unique ability of transforming chemical selenium, such as sodium selenite, into a biologically active form, which mitigates its toxic effects on the human body. The transformation product of this process, selenomethionine, can be safely and effectively absorbed...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/29/1/235 |
| _version_ | 1797358372418224128 |
|---|---|
| author | Xiao Li Ling Shi Panshu Song Wei Cai Ximing Luo Bo Zhao |
| author_facet | Xiao Li Ling Shi Panshu Song Wei Cai Ximing Luo Bo Zhao |
| author_sort | Xiao Li |
| collection | DOAJ |
| description | Selenium-enriched yeast possesses the unique ability of transforming chemical selenium, such as sodium selenite, into a biologically active form, which mitigates its toxic effects on the human body. The transformation product of this process, selenomethionine, can be safely and effectively absorbed and utilized by the human body; hence, it has been spiked into a selenium-enriched supplement. This study employs two distinct measurement strategies to determine the selenomethionine content in two candidate reference materials, a selenium-enriched yeast powder and supplement, using both organic and inorganic mass spectrometry. The concentrations of selenomethionine in the selenium-enriched yeast were determined using HPLC-ICP-MS and HPLC- ESI-MS/MS, with mass fractions measured at 718 mg SeMet kg<sup>−1</sup> and 715 mg SeMet kg<sup>−1</sup>, respectively. Notably, both methods yielded consistent results for the selenium supplement, with a selenomethionine mass fraction of 59 mg SeMet kg<sup>−1</sup>. Ultimately, the certified values of these candidate reference materials were determined as 716 mg kg<sup>−1</sup> and 59 mg SeMet kg<sup>−1</sup> with expanded uncertainties of 36 mg SeMet kg<sup>−1</sup> (<i>k</i> = 2) and 5 mg SeMet kg<sup>−1</sup> (<i>k</i> = 2), respectively. The development of these candidate reference materials serves as a valuable reference for diverse methods aiming to determine the value of organic selenium speciation in complex food substrates. |
| first_indexed | 2024-03-08T15:01:02Z |
| format | Article |
| id | doaj.art-0c7c5bb7962f4baa984c8a6c917fbb34 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T15:01:02Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0c7c5bb7962f4baa984c8a6c917fbb342024-01-10T15:04:35ZengMDPI AGMolecules1420-30492024-01-0129123510.3390/molecules29010235Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement StrategiesXiao Li0Ling Shi1Panshu Song2Wei Cai3Ximing Luo4Bo Zhao5School of Ocean Sciences, China University of Geosciences (Beijing), Beijing 100083, ChinaDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, ChinaDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, ChinaDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, ChinaSchool of Ocean Sciences, China University of Geosciences (Beijing), Beijing 100083, ChinaDivision of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, ChinaSelenium-enriched yeast possesses the unique ability of transforming chemical selenium, such as sodium selenite, into a biologically active form, which mitigates its toxic effects on the human body. The transformation product of this process, selenomethionine, can be safely and effectively absorbed and utilized by the human body; hence, it has been spiked into a selenium-enriched supplement. This study employs two distinct measurement strategies to determine the selenomethionine content in two candidate reference materials, a selenium-enriched yeast powder and supplement, using both organic and inorganic mass spectrometry. The concentrations of selenomethionine in the selenium-enriched yeast were determined using HPLC-ICP-MS and HPLC- ESI-MS/MS, with mass fractions measured at 718 mg SeMet kg<sup>−1</sup> and 715 mg SeMet kg<sup>−1</sup>, respectively. Notably, both methods yielded consistent results for the selenium supplement, with a selenomethionine mass fraction of 59 mg SeMet kg<sup>−1</sup>. Ultimately, the certified values of these candidate reference materials were determined as 716 mg kg<sup>−1</sup> and 59 mg SeMet kg<sup>−1</sup> with expanded uncertainties of 36 mg SeMet kg<sup>−1</sup> (<i>k</i> = 2) and 5 mg SeMet kg<sup>−1</sup> (<i>k</i> = 2), respectively. The development of these candidate reference materials serves as a valuable reference for diverse methods aiming to determine the value of organic selenium speciation in complex food substrates.https://www.mdpi.com/1420-3049/29/1/235selenium speciationselenomethioninecertified reference materialHPLC-ICP-MSHPLC-ESI-MS/MS |
| spellingShingle | Xiao Li Ling Shi Panshu Song Wei Cai Ximing Luo Bo Zhao Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement Strategies Molecules selenium speciation selenomethionine certified reference material HPLC-ICP-MS HPLC-ESI-MS/MS |
| title | Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement Strategies |
| title_full | Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement Strategies |
| title_fullStr | Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement Strategies |
| title_full_unstemmed | Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement Strategies |
| title_short | Certification of New Selenium-Enriched Yeast and Supplement Reference Materials for Selenomethionine Using Two Independent Measurement Strategies |
| title_sort | certification of new selenium enriched yeast and supplement reference materials for selenomethionine using two independent measurement strategies |
| topic | selenium speciation selenomethionine certified reference material HPLC-ICP-MS HPLC-ESI-MS/MS |
| url | https://www.mdpi.com/1420-3049/29/1/235 |
| work_keys_str_mv | AT xiaoli certificationofnewseleniumenrichedyeastandsupplementreferencematerialsforselenomethionineusingtwoindependentmeasurementstrategies AT lingshi certificationofnewseleniumenrichedyeastandsupplementreferencematerialsforselenomethionineusingtwoindependentmeasurementstrategies AT panshusong certificationofnewseleniumenrichedyeastandsupplementreferencematerialsforselenomethionineusingtwoindependentmeasurementstrategies AT weicai certificationofnewseleniumenrichedyeastandsupplementreferencematerialsforselenomethionineusingtwoindependentmeasurementstrategies AT ximingluo certificationofnewseleniumenrichedyeastandsupplementreferencematerialsforselenomethionineusingtwoindependentmeasurementstrategies AT bozhao certificationofnewseleniumenrichedyeastandsupplementreferencematerialsforselenomethionineusingtwoindependentmeasurementstrategies |