Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental Variations
Seed dormancy and timing of its release is an important developmental transition determining the survival of individuals, populations, and species in variable environments. <i>Medicago truncatula</i> was used as a model to study physical seed dormancy at the ecological and genetics level...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/4/503 |
| _version_ | 1827718671773990912 |
|---|---|
| author | Juan Pablo Renzi Martin Duchoslav Jan Brus Iveta Hradilová Vilém Pechanec Tadeáš Václavek Jitka Machalová Karel Hron Jerome Verdier Petr Smýkal |
| author_facet | Juan Pablo Renzi Martin Duchoslav Jan Brus Iveta Hradilová Vilém Pechanec Tadeáš Václavek Jitka Machalová Karel Hron Jerome Verdier Petr Smýkal |
| author_sort | Juan Pablo Renzi |
| collection | DOAJ |
| description | Seed dormancy and timing of its release is an important developmental transition determining the survival of individuals, populations, and species in variable environments. <i>Medicago truncatula</i> was used as a model to study physical seed dormancy at the ecological and genetics level. The effect of alternating temperatures, as one of the causes releasing physical seed dormancy, was tested in 178 <i>M. truncatula</i> accessions over three years. Several coefficients of dormancy release were related to environmental variables. Dormancy varied greatly (4–100%) across accessions as well as year of experiment. We observed overall higher physical dormancy release under more alternating temperatures (35/15 °C) in comparison with less alternating ones (25/15 °C). Accessions from more arid climates released dormancy under higher experimental temperature alternations more than accessions originating from less arid environments. The plasticity of physical dormancy can probably distribute the germination through the year and act as a bet-hedging strategy in arid environments. On the other hand, a slight increase in physical dormancy was observed in accessions from environments with higher among-season temperature variation. Genome-wide association analysis identified 136 candidate genes related to secondary metabolite synthesis, hormone regulation, and modification of the cell wall. The activity of these genes might mediate seed coat permeability and, ultimately, imbibition and germination. |
| first_indexed | 2024-03-10T20:27:44Z |
| format | Article |
| id | doaj.art-0c7e68ff039646859119c0338046608e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:27:44Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-0c7e68ff039646859119c0338046608e2023-11-19T21:36:50ZengMDPI AGPlants2223-77472020-04-019450310.3390/plants9040503Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental VariationsJuan Pablo Renzi0Martin Duchoslav1Jan Brus2Iveta Hradilová3Vilém Pechanec4Tadeáš Václavek5Jitka Machalová6Karel Hron7Jerome Verdier8Petr Smýkal9Instituto Nacional de Tecnología Agropecuaria, Hilario Ascasubi 8142, ArgentinaDepartment of Botany, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech RepublicDepartment of Geoinformatics, Palacký University, 17. listopadu 50, 771 46 Olomouc, Czech RepublicDepartment of Botany, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech RepublicDepartment of Geoinformatics, Palacký University, 17. listopadu 50, 771 46 Olomouc, Czech RepublicDepartment of Mathematical Analysis and Applications of Mathematics, Palacký University, 17. listopadu 12, 771 46 Olomouc, Czech RepublicDepartment of Mathematical Analysis and Applications of Mathematics, Palacký University, 17. listopadu 12, 771 46 Olomouc, Czech RepublicDepartment of Mathematical Analysis and Applications of Mathematics, Palacký University, 17. listopadu 12, 771 46 Olomouc, Czech RepublicUMR 1345 Institut de Recherche en Horticulture et Semences, Agrocampus Ouest, INRA, Université d’Angers, SFR 4207 QUASAV, 49070 Beaucouzé, FranceDepartment of Botany, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech RepublicSeed dormancy and timing of its release is an important developmental transition determining the survival of individuals, populations, and species in variable environments. <i>Medicago truncatula</i> was used as a model to study physical seed dormancy at the ecological and genetics level. The effect of alternating temperatures, as one of the causes releasing physical seed dormancy, was tested in 178 <i>M. truncatula</i> accessions over three years. Several coefficients of dormancy release were related to environmental variables. Dormancy varied greatly (4–100%) across accessions as well as year of experiment. We observed overall higher physical dormancy release under more alternating temperatures (35/15 °C) in comparison with less alternating ones (25/15 °C). Accessions from more arid climates released dormancy under higher experimental temperature alternations more than accessions originating from less arid environments. The plasticity of physical dormancy can probably distribute the germination through the year and act as a bet-hedging strategy in arid environments. On the other hand, a slight increase in physical dormancy was observed in accessions from environments with higher among-season temperature variation. Genome-wide association analysis identified 136 candidate genes related to secondary metabolite synthesis, hormone regulation, and modification of the cell wall. The activity of these genes might mediate seed coat permeability and, ultimately, imbibition and germination.https://www.mdpi.com/2223-7747/9/4/503association mappingclimate adaptationgerminationgenomicslegumes<i>Medicago</i> |
| spellingShingle | Juan Pablo Renzi Martin Duchoslav Jan Brus Iveta Hradilová Vilém Pechanec Tadeáš Václavek Jitka Machalová Karel Hron Jerome Verdier Petr Smýkal Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental Variations Plants association mapping climate adaptation germination genomics legumes <i>Medicago</i> |
| title | Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental Variations |
| title_full | Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental Variations |
| title_fullStr | Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental Variations |
| title_full_unstemmed | Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental Variations |
| title_short | Physical Dormancy Release in <i>Medicago truncatula</i> Seeds Is Related to Environmental Variations |
| title_sort | physical dormancy release in i medicago truncatula i seeds is related to environmental variations |
| topic | association mapping climate adaptation germination genomics legumes <i>Medicago</i> |
| url | https://www.mdpi.com/2223-7747/9/4/503 |
| work_keys_str_mv | AT juanpablorenzi physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT martinduchoslav physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT janbrus physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT ivetahradilova physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT vilempechanec physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT tadeasvaclavek physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT jitkamachalova physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT karelhron physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT jeromeverdier physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations AT petrsmykal physicaldormancyreleaseinimedicagotruncatulaiseedsisrelatedtoenvironmentalvariations |