Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype
Common Variable Immunodeficiency (CVID) is the most frequent symptomatic immune disorder characterized by reduced serum immunoglobulins. Patients often suffer from infectious and serious non-infectious complications which impact their life tremendously. The monogenic cause has been revealed in a min...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01914/full |
| _version_ | 1819160870600572928 |
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| author | Kristýna Fiedorová Kristýna Fiedorová Kristýna Fiedorová Matěj Radvanský Juraj Bosák Hana Grombiříková Hana Grombiříková Eva Němcová Pavlína Králíčková Michaela Černochová Iva Kotásková Iva Kotásková Iva Kotásková Matej Lexa Jiří Litzman Jiří Litzman David Šmajs Tomáš Freiberger Tomáš Freiberger Tomáš Freiberger |
| author_facet | Kristýna Fiedorová Kristýna Fiedorová Kristýna Fiedorová Matěj Radvanský Juraj Bosák Hana Grombiříková Hana Grombiříková Eva Němcová Pavlína Králíčková Michaela Černochová Iva Kotásková Iva Kotásková Iva Kotásková Matej Lexa Jiří Litzman Jiří Litzman David Šmajs Tomáš Freiberger Tomáš Freiberger Tomáš Freiberger |
| author_sort | Kristýna Fiedorová |
| collection | DOAJ |
| description | Common Variable Immunodeficiency (CVID) is the most frequent symptomatic immune disorder characterized by reduced serum immunoglobulins. Patients often suffer from infectious and serious non-infectious complications which impact their life tremendously. The monogenic cause has been revealed in a minority of patients so far, indicating the role of multiple genes and environmental factors in CVID etiology. Using 16S and ITS rRNA amplicon sequencing, we analyzed the bacterial and fungal gut microbiota, respectively, in a group of 55 participants constituting of CVID patients and matched healthy controls including 16 case-control pairs living in the same household, to explore possible associations between gut microbiota composition and disease phenotype. We revealed less diverse and significantly altered bacterial but not fungal gut microbiota in CVID patients, which additionally appeared to be associated with a more severe disease phenotype. The factor of sharing the same household impacted both bacterial and fungal microbiome data significantly, although not as strongly as CVID diagnosis in bacterial assessment. Overall, our results suggest that gut bacterial microbiota is altered in CVID patients and may be one of the missing environmental drivers contributing to some of the symptoms and disease severity. Paired samples serving as controls will provide a better resolution between disease-related dysbiosis and other environmental confounders in future studies. |
| first_indexed | 2024-12-22T17:03:19Z |
| format | Article |
| id | doaj.art-0c8058a555d748fd9995566c1270d85b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T17:03:19Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-0c8058a555d748fd9995566c1270d85b2022-12-21T18:19:17ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-08-011010.3389/fimmu.2019.01914473430Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) PhenotypeKristýna Fiedorová0Kristýna Fiedorová1Kristýna Fiedorová2Matěj Radvanský3Juraj Bosák4Hana Grombiříková5Hana Grombiříková6Eva Němcová7Pavlína Králíčková8Michaela Černochová9Iva Kotásková10Iva Kotásková11Iva Kotásková12Matej Lexa13Jiří Litzman14Jiří Litzman15David Šmajs16Tomáš Freiberger17Tomáš Freiberger18Tomáš Freiberger19Centre for Cardiovascular Surgery and Transplantation, Brno, CzechiaCentral European Institute of Technology, Masaryk University, Brno, CzechiaDepartment of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaFaculty of Informatics, Masaryk University, Brno, CzechiaDepartment of Biology, Faculty of Medicine, Masaryk University, Brno, CzechiaCentre for Cardiovascular Surgery and Transplantation, Brno, CzechiaDepartment of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaCentre for Cardiovascular Surgery and Transplantation, Brno, CzechiaDepartment of Allergology and Clinical Immunology, Faculty of Medicine, Charles University and University Hospital in Hradec Kralove, Hradec Kralove, CzechiaCentre for Cardiovascular Surgery and Transplantation, Brno, CzechiaCentre for Cardiovascular Surgery and Transplantation, Brno, CzechiaCentral European Institute of Technology, Masaryk University, Brno, CzechiaDepartment of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaFaculty of Informatics, Masaryk University, Brno, CzechiaDepartment of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Clinical Immunology and Allergology, St. Anne's University Hospital in Brno, Brno, CzechiaDepartment of Biology, Faculty of Medicine, Masaryk University, Brno, CzechiaCentre for Cardiovascular Surgery and Transplantation, Brno, CzechiaCentral European Institute of Technology, Masaryk University, Brno, CzechiaDepartment of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, CzechiaCommon Variable Immunodeficiency (CVID) is the most frequent symptomatic immune disorder characterized by reduced serum immunoglobulins. Patients often suffer from infectious and serious non-infectious complications which impact their life tremendously. The monogenic cause has been revealed in a minority of patients so far, indicating the role of multiple genes and environmental factors in CVID etiology. Using 16S and ITS rRNA amplicon sequencing, we analyzed the bacterial and fungal gut microbiota, respectively, in a group of 55 participants constituting of CVID patients and matched healthy controls including 16 case-control pairs living in the same household, to explore possible associations between gut microbiota composition and disease phenotype. We revealed less diverse and significantly altered bacterial but not fungal gut microbiota in CVID patients, which additionally appeared to be associated with a more severe disease phenotype. The factor of sharing the same household impacted both bacterial and fungal microbiome data significantly, although not as strongly as CVID diagnosis in bacterial assessment. Overall, our results suggest that gut bacterial microbiota is altered in CVID patients and may be one of the missing environmental drivers contributing to some of the symptoms and disease severity. Paired samples serving as controls will provide a better resolution between disease-related dysbiosis and other environmental confounders in future studies.https://www.frontiersin.org/article/10.3389/fimmu.2019.01914/fullCVIDIgAgut microbiotagut microbiomegut mycobiotagut mycobiome |
| spellingShingle | Kristýna Fiedorová Kristýna Fiedorová Kristýna Fiedorová Matěj Radvanský Juraj Bosák Hana Grombiříková Hana Grombiříková Eva Němcová Pavlína Králíčková Michaela Černochová Iva Kotásková Iva Kotásková Iva Kotásková Matej Lexa Jiří Litzman Jiří Litzman David Šmajs Tomáš Freiberger Tomáš Freiberger Tomáš Freiberger Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype Frontiers in Immunology CVID IgA gut microbiota gut microbiome gut mycobiota gut mycobiome |
| title | Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype |
| title_full | Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype |
| title_fullStr | Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype |
| title_full_unstemmed | Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype |
| title_short | Bacterial but Not Fungal Gut Microbiota Alterations Are Associated With Common Variable Immunodeficiency (CVID) Phenotype |
| title_sort | bacterial but not fungal gut microbiota alterations are associated with common variable immunodeficiency cvid phenotype |
| topic | CVID IgA gut microbiota gut microbiome gut mycobiota gut mycobiome |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01914/full |
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