FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTS
ABSTRACT BACKGROUND: Colorectal bleeding is a warning sign that may be identified by fecal occult blood testing. A positive fecal occult blood test result requires a subsequent colonoscopy, a costly and invasive examination. Therefore, the use of diagnostic tests with optimal sensitivity and speci...
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| Format: | Article |
| Language: | English |
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Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE)
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| Series: | Arquivos de Gastroenterologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032018000200128&lng=en&tlng=en |
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| author | Luana Vilarinho BORGES Rejane MATTAR Joyce Matie Kinoshita da SILVA Ana Luiza Werneck da SILVA Flair José CARRILHO Cláudio Lyoiti HASHIMOTO |
| author_facet | Luana Vilarinho BORGES Rejane MATTAR Joyce Matie Kinoshita da SILVA Ana Luiza Werneck da SILVA Flair José CARRILHO Cláudio Lyoiti HASHIMOTO |
| author_sort | Luana Vilarinho BORGES |
| collection | DOAJ |
| description | ABSTRACT BACKGROUND: Colorectal bleeding is a warning sign that may be identified by fecal occult blood testing. A positive fecal occult blood test result requires a subsequent colonoscopy, a costly and invasive examination. Therefore, the use of diagnostic tests with optimal sensitivity and specificity is warranted. In this study, we evaluated four different fecal occult blood tests in 176 patients undergoing colonoscopy and compared their results. OBJECTIVE: To assess the sensitivity, specificity and predictive values of chemical and immunochemical fecal occult blood tests in patients undergoing colonoscopy and to evaluate the degree of concordance between the tests and colonoscopy. METHODS: Patients with indications for colonoscopy also underwent fecal occult blood testing by chemical (toluidine test) and immunochemical methods, employing three commercially available kits. Based on the endoscopic findings, the colonoscopy was rated as positive or negative for colorectal bleeding. The degree of concordance between the fecal occult blood tests and the colonoscopy was evaluated by the kappa index. RESULTS: Forty-four (25%) colonoscopies were categorized as positive for colorectal bleeding. The toluidine test presented lower concordance than the immunochemical tests, which showed moderate concordance with the colonoscopy. The toluidine test had the least sensitivity, specificity, and positive and negative predictive values. CONCLUSION: The immunochemical fecal occult blood tests showed greater sensitivity, specificity and predictive values in detecting colorectal bleeding. The immunochemical tests had superior indexes of agreement with colonoscopy compared to the toluidine test. |
| first_indexed | 2024-04-13T23:41:22Z |
| format | Article |
| id | doaj.art-0c80c8284c8843dd914606ddb5430bd2 |
| institution | Directory Open Access Journal |
| issn | 1678-4219 |
| language | English |
| last_indexed | 2024-04-13T23:41:22Z |
| publisher | Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE) |
| record_format | Article |
| series | Arquivos de Gastroenterologia |
| spelling | doaj.art-0c80c8284c8843dd914606ddb5430bd22022-12-22T02:24:29ZengInstituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE)Arquivos de Gastroenterologia1678-421955212813210.1590/s0004-2803.201800000-22S0004-28032018000200128FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTSLuana Vilarinho BORGESRejane MATTARJoyce Matie Kinoshita da SILVAAna Luiza Werneck da SILVAFlair José CARRILHOCláudio Lyoiti HASHIMOTOABSTRACT BACKGROUND: Colorectal bleeding is a warning sign that may be identified by fecal occult blood testing. A positive fecal occult blood test result requires a subsequent colonoscopy, a costly and invasive examination. Therefore, the use of diagnostic tests with optimal sensitivity and specificity is warranted. In this study, we evaluated four different fecal occult blood tests in 176 patients undergoing colonoscopy and compared their results. OBJECTIVE: To assess the sensitivity, specificity and predictive values of chemical and immunochemical fecal occult blood tests in patients undergoing colonoscopy and to evaluate the degree of concordance between the tests and colonoscopy. METHODS: Patients with indications for colonoscopy also underwent fecal occult blood testing by chemical (toluidine test) and immunochemical methods, employing three commercially available kits. Based on the endoscopic findings, the colonoscopy was rated as positive or negative for colorectal bleeding. The degree of concordance between the fecal occult blood tests and the colonoscopy was evaluated by the kappa index. RESULTS: Forty-four (25%) colonoscopies were categorized as positive for colorectal bleeding. The toluidine test presented lower concordance than the immunochemical tests, which showed moderate concordance with the colonoscopy. The toluidine test had the least sensitivity, specificity, and positive and negative predictive values. CONCLUSION: The immunochemical fecal occult blood tests showed greater sensitivity, specificity and predictive values in detecting colorectal bleeding. The immunochemical tests had superior indexes of agreement with colonoscopy compared to the toluidine test.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032018000200128&lng=en&tlng=enOccult bloodToluidinesImmunologic testsColonoscopySensitivity and specificityPredictive value of tests |
| spellingShingle | Luana Vilarinho BORGES Rejane MATTAR Joyce Matie Kinoshita da SILVA Ana Luiza Werneck da SILVA Flair José CARRILHO Cláudio Lyoiti HASHIMOTO FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTS Arquivos de Gastroenterologia Occult blood Toluidines Immunologic tests Colonoscopy Sensitivity and specificity Predictive value of tests |
| title | FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTS |
| title_full | FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTS |
| title_fullStr | FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTS |
| title_full_unstemmed | FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTS |
| title_short | FECAL OCCULT BLOOD: A COMPARISON OF CHEMICAL AND IMMUNOCHEMICAL TESTS |
| title_sort | fecal occult blood a comparison of chemical and immunochemical tests |
| topic | Occult blood Toluidines Immunologic tests Colonoscopy Sensitivity and specificity Predictive value of tests |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032018000200128&lng=en&tlng=en |
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