Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry
The research presents a comparative metabolomic study of extracts of <i>Vigna unguiculata</i> seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/16/2147 |
| _version_ | 1827598589812015104 |
|---|---|
| author | Mayya P. Razgonova Marina O. Burlyaeva Yulia N. Zinchenko Ekaterina A. Krylova Olga A. Chunikhina Natalia M. Ivanova Alexander M. Zakharenko Kirill S. Golokhvast |
| author_facet | Mayya P. Razgonova Marina O. Burlyaeva Yulia N. Zinchenko Ekaterina A. Krylova Olga A. Chunikhina Natalia M. Ivanova Alexander M. Zakharenko Kirill S. Golokhvast |
| author_sort | Mayya P. Razgonova |
| collection | DOAJ |
| description | The research presents a comparative metabolomic study of extracts of <i>Vigna unguiculata</i> seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups <i>sesquipedalis</i> (vegetable accessions) and <i>unguiculata</i> (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in <i>V. unguiculata</i> seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group <i>sesquipedalis</i>, and 24 in <i>unguiculata</i>. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China. |
| first_indexed | 2024-03-09T03:56:11Z |
| format | Article |
| id | doaj.art-0c87ac9448a0494db4b7ffed05017ac6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:56:11Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-0c87ac9448a0494db4b7ffed05017ac62023-12-03T14:19:18ZengMDPI AGPlants2223-77472022-08-011116214710.3390/plants11162147Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass SpectrometryMayya P. Razgonova0Marina O. Burlyaeva1Yulia N. Zinchenko2Ekaterina A. Krylova3Olga A. Chunikhina4Natalia M. Ivanova5Alexander M. Zakharenko6Kirill S. Golokhvast7N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, RussiaDepartment of Botany, Saint-Petersburg State University, 199034 Saint-Petersburg, RussiaSiberian Federal Scientific Centre of Agrobiotechnology RAS, 633501 Krasnoobsk, RussiaN.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Saint-Petersburg, RussiaThe research presents a comparative metabolomic study of extracts of <i>Vigna unguiculata</i> seed samples from the collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Analyzed samples related to different areas of use in agricultural production, belonging to different cultivar groups <i>sesquipedalis</i> (vegetable accessions) and <i>unguiculata</i> (grain accessions). Metabolome analysis was performed by liquid chromatography combined with ion trap mass spectrometry. Substances were localized in seeds using confocal and laser microscopy. As a result, 49 bioactive compounds were identified: flavonols, flavones, flavan-3-ols, anthocyanidin, phenolic acids, amino acids, monocarboxylic acids, aminobenzoic acids, fatty acids, lignans, carotenoid, sapogenins, steroids, etc. Steroidal alkaloids were identified in <i>V. unguiculata</i> seeds for the first time. The seed coat (palisade epidermis and parenchyma) is the richest in phenolic compounds. Comparison of seeds of varieties of different directions of use in terms of the number of bioactive substances identified revealed a significant superiority of vegetable accessions over grain ones in this indicator, 36 compounds were found in samples from cultivar group <i>sesquipedalis</i>, and 24 in <i>unguiculata</i>. The greatest variety of bioactive compounds was found in the vegetable accession k-640 from China.https://www.mdpi.com/2223-7747/11/16/2147<i>Vigna unguiculata</i>tandem mass spectrometrymetaboliteslocal landracevegetable cultivargrain cultivar |
| spellingShingle | Mayya P. Razgonova Marina O. Burlyaeva Yulia N. Zinchenko Ekaterina A. Krylova Olga A. Chunikhina Natalia M. Ivanova Alexander M. Zakharenko Kirill S. Golokhvast Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry Plants <i>Vigna unguiculata</i> tandem mass spectrometry metabolites local landrace vegetable cultivar grain cultivar |
| title | Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry |
| title_full | Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry |
| title_fullStr | Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry |
| title_full_unstemmed | Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry |
| title_short | Identification and Spatial Distribution of Bioactive Compounds in Seeds <i>Vigna unguiculata</i> (L.) Walp. by Laser Microscopy and Tandem Mass Spectrometry |
| title_sort | identification and spatial distribution of bioactive compounds in seeds i vigna unguiculata i l walp by laser microscopy and tandem mass spectrometry |
| topic | <i>Vigna unguiculata</i> tandem mass spectrometry metabolites local landrace vegetable cultivar grain cultivar |
| url | https://www.mdpi.com/2223-7747/11/16/2147 |
| work_keys_str_mv | AT mayyaprazgonova identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry AT marinaoburlyaeva identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry AT yulianzinchenko identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry AT ekaterinaakrylova identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry AT olgaachunikhina identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry AT nataliamivanova identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry AT alexandermzakharenko identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry AT kirillsgolokhvast identificationandspatialdistributionofbioactivecompoundsinseedsivignaunguiculatailwalpbylasermicroscopyandtandemmassspectrometry |