Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology
Objective The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC). Methods A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different group...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2018-11-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-18-0018.pdf |
| _version_ | 1819139655886438400 |
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| author | Ramasamy Selvam Marimuthu Saravanakumar Subramaniyam Suresh CV Chandrasekeran D’Souza Prashanth |
| author_facet | Ramasamy Selvam Marimuthu Saravanakumar Subramaniyam Suresh CV Chandrasekeran D’Souza Prashanth |
| author_sort | Ramasamy Selvam |
| collection | DOAJ |
| description | Objective The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC). Methods A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different groups in three experiments. In experiment 1, G1 and G2 served as normal controls and were fed a basal diet with 100% soybean meal (SBM) as a major protein source supplemented with and without SCC, respectively. In G3, G4, G5, and G6 groups, SBM was replaced at 25%, 50%, 75%, and 100% by soy protein isolate (SPI) to induce a graded level of choline deficiency. In experiment 2, PHF (500 and 1,000 g/ton) in comparison with SCC (1,000 g/ton) were evaluated. In experiment 3, dose-response of PHF (200, 400, and 500 g/ton) with SCC (400 g/ton) was determined. Results Replacement of SBM by SPI produced a linear decrease in body weight gain (BWG) with a poor feed conversion ratio (FCR). 25% SBM replacement by SPI yielded an optimum negative impact on BWG and FCR; hence, it is considered for further studies. In experiment 2, PHF (500 and 1,000 g/ton) and SCC (1,000 g/ton) showed a similar performance in BWG, FCR and relative liver weight. In experiment 3, PHF produced an optimum efficacy at 400 g/ton and was comparable to SCC in the restoration of serum aspartate aminotransferase activity, abdominal fat, breast muscle lipid content and liver histopathological abnormalities. Conclusion Replacement of SBM by SPI caused choline deficiency characterised by worsening of BWG, FCR, elevation in liver enzymes and histopathological changes indicating fatty liver. CDM was found valid for evaluating SCC and PHF. It is concluded that PHF has the potential to mimic biological activities of SCC through the restoration of negative effects caused by CDM. |
| first_indexed | 2024-12-22T11:26:07Z |
| format | Article |
| id | doaj.art-0c8819093aa044cabda2be5ab6656114 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-22T11:26:07Z |
| publishDate | 2018-11-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-0c8819093aa044cabda2be5ab66561142022-12-21T18:27:45ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172018-11-0131111795180610.5713/ajas.18.001823976Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathologyRamasamy Selvam0Marimuthu Saravanakumar1Subramaniyam Suresh2CV Chandrasekeran3D’Souza Prashanth4 Animal Health Science, R&D Centre, Natural Remedies Private Limited., Bangalore, Karnataka - 560 100, India Animal Health Science, R&D Centre, Natural Remedies Private Limited., Bangalore, Karnataka - 560 100, India Animal Health Science, R&D Centre, Natural Remedies Private Limited., Bangalore, Karnataka - 560 100, India R&D Biology, R&D Centre, Natural Remedies Private Limited., Bangalore, Karnataka - 560 100, India Formulation & Development, R&D Centre, Natural Remedies Private Limited., Bangalore, Karnataka - 560 100, IndiaObjective The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC). Methods A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different groups in three experiments. In experiment 1, G1 and G2 served as normal controls and were fed a basal diet with 100% soybean meal (SBM) as a major protein source supplemented with and without SCC, respectively. In G3, G4, G5, and G6 groups, SBM was replaced at 25%, 50%, 75%, and 100% by soy protein isolate (SPI) to induce a graded level of choline deficiency. In experiment 2, PHF (500 and 1,000 g/ton) in comparison with SCC (1,000 g/ton) were evaluated. In experiment 3, dose-response of PHF (200, 400, and 500 g/ton) with SCC (400 g/ton) was determined. Results Replacement of SBM by SPI produced a linear decrease in body weight gain (BWG) with a poor feed conversion ratio (FCR). 25% SBM replacement by SPI yielded an optimum negative impact on BWG and FCR; hence, it is considered for further studies. In experiment 2, PHF (500 and 1,000 g/ton) and SCC (1,000 g/ton) showed a similar performance in BWG, FCR and relative liver weight. In experiment 3, PHF produced an optimum efficacy at 400 g/ton and was comparable to SCC in the restoration of serum aspartate aminotransferase activity, abdominal fat, breast muscle lipid content and liver histopathological abnormalities. Conclusion Replacement of SBM by SPI caused choline deficiency characterised by worsening of BWG, FCR, elevation in liver enzymes and histopathological changes indicating fatty liver. CDM was found valid for evaluating SCC and PHF. It is concluded that PHF has the potential to mimic biological activities of SCC through the restoration of negative effects caused by CDM.http://www.ajas.info/upload/pdf/ajas-18-0018.pdfCholine Deficiency ModelChickensPolyherbal FormulationCholine Chloride |
| spellingShingle | Ramasamy Selvam Marimuthu Saravanakumar Subramaniyam Suresh CV Chandrasekeran D’Souza Prashanth Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology Asian-Australasian Journal of Animal Sciences Choline Deficiency Model Chickens Polyherbal Formulation Choline Chloride |
| title | Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology |
| title_full | Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology |
| title_fullStr | Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology |
| title_full_unstemmed | Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology |
| title_short | Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology |
| title_sort | evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers implications on zootechnical parameters serum biochemistry and liver histopathology |
| topic | Choline Deficiency Model Chickens Polyherbal Formulation Choline Chloride |
| url | http://www.ajas.info/upload/pdf/ajas-18-0018.pdf |
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