Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope Labeling
The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of <sup>13</sup>C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom <i>Chaetocero...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/797 |
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| author | Marine Remize Frédéric Planchon Ai Ning Loh Fabienne Le Grand Antoine Bideau Nelly Le Goic Elodie Fleury Philippe Miner Rudolph Corvaisier Aswani Volety Philippe Soudant |
| author_facet | Marine Remize Frédéric Planchon Ai Ning Loh Fabienne Le Grand Antoine Bideau Nelly Le Goic Elodie Fleury Philippe Miner Rudolph Corvaisier Aswani Volety Philippe Soudant |
| author_sort | Marine Remize |
| collection | DOAJ |
| description | The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of <sup>13</sup>C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom <i>Chaetoceros muelleri</i> for 24 h. <i>Chaetoceros muelleri</i> preferentially and quickly incorporated <sup>13</sup>C into C<sub>18</sub> PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly <sup>13</sup>C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6 (via 18:3n-6 and 20:4n-6) synthesis pathways as well as the alternative ω-3 desaturase pathway (via 20:4n-6). In addition, as FA from polar lipids were generally more enriched in <sup>13</sup>C than FA from neutral lipids, particularly for 18:1n-9, 18:2n-6 and 18:3n-6, the existence of acyl-editing mechanisms and connectivity between polar and neutral lipid fatty acid pools were also hypothesized. Because 16:3n-4 and 20:5n-3 presented the same concentration and enrichment dynamics, a structural and metabolic link was proposed between these two PUFAs in <i>C. muelleri</i>. |
| first_indexed | 2024-03-10T19:40:19Z |
| format | Article |
| id | doaj.art-0c88258ef9334a2f9e21691adb677b15 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:40:19Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-0c88258ef9334a2f9e21691adb677b152023-11-20T01:17:18ZengMDPI AGBiomolecules2218-273X2020-05-0110579710.3390/biom10050797Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope LabelingMarine Remize0Frédéric Planchon1Ai Ning Loh2Fabienne Le Grand3Antoine Bideau4Nelly Le Goic5Elodie Fleury6Philippe Miner7Rudolph Corvaisier8Aswani Volety9Philippe Soudant10Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, FranceUniv Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, FranceDepartment of Earth and Ocean Sciences, Center for Marine Science, University of North Carolina Wilmington, 5600 Marvin K. Moss Ln, Wilmington, NC 28403, USAUniv Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, FranceUniv Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, FranceUniv Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, FranceInstitut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 UBO/CNRS/IRD/IFREMER, CS 10070, 1625 Route de Sainte-Anne, 29280 Plouzane, FranceInstitut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 UBO/CNRS/IRD/IFREMER, CS 10070, 1625 Route de Sainte-Anne, 29280 Plouzane, FranceUniv Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, FranceDepartment of Biology, Elon University, 50 Campus Drive, Elon, NC 27244, USAUniv Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, FranceThe present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of <sup>13</sup>C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom <i>Chaetoceros muelleri</i> for 24 h. <i>Chaetoceros muelleri</i> preferentially and quickly incorporated <sup>13</sup>C into C<sub>18</sub> PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly <sup>13</sup>C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6 (via 18:3n-6 and 20:4n-6) synthesis pathways as well as the alternative ω-3 desaturase pathway (via 20:4n-6). In addition, as FA from polar lipids were generally more enriched in <sup>13</sup>C than FA from neutral lipids, particularly for 18:1n-9, 18:2n-6 and 18:3n-6, the existence of acyl-editing mechanisms and connectivity between polar and neutral lipid fatty acid pools were also hypothesized. Because 16:3n-4 and 20:5n-3 presented the same concentration and enrichment dynamics, a structural and metabolic link was proposed between these two PUFAs in <i>C. muelleri</i>.https://www.mdpi.com/2218-273X/10/5/797synthesis pathwaydiatom20:5n-3 (EPA)<i>Chaetoceros muelleri</i>acyl-editing mechanismcompound-specific isotope analysis |
| spellingShingle | Marine Remize Frédéric Planchon Ai Ning Loh Fabienne Le Grand Antoine Bideau Nelly Le Goic Elodie Fleury Philippe Miner Rudolph Corvaisier Aswani Volety Philippe Soudant Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope Labeling Biomolecules synthesis pathway diatom 20:5n-3 (EPA) <i>Chaetoceros muelleri</i> acyl-editing mechanism compound-specific isotope analysis |
| title | Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope Labeling |
| title_full | Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope Labeling |
| title_fullStr | Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope Labeling |
| title_full_unstemmed | Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope Labeling |
| title_short | Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom <i>Chaetoceros Muelleri</i> Using <sup>13</sup>C-Isotope Labeling |
| title_sort | study of synthesis pathways of the essential polyunsaturated fatty acid 20 5n 3 in the diatom i chaetoceros muelleri i using sup 13 sup c isotope labeling |
| topic | synthesis pathway diatom 20:5n-3 (EPA) <i>Chaetoceros muelleri</i> acyl-editing mechanism compound-specific isotope analysis |
| url | https://www.mdpi.com/2218-273X/10/5/797 |
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