Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa
Abstract Background P. aeruginosa is the primary source of hospital-acquired infections. Unfortunately, antibiotic resistance is growing to precariously high levels, making the infections by this pathogen life-threatening and hard to cure. One possible alternative to antibiotics is to use phages. Ho...
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| Format: | Article |
| Language: | English |
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BMC
2021-05-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-021-02197-z |
| _version_ | 1818573633349484544 |
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| author | Bahareh Lashtoo Aghaee Mohammadali Khan Mirzaei Mohammad Yousef Alikhani Ali Mojtahedi |
| author_facet | Bahareh Lashtoo Aghaee Mohammadali Khan Mirzaei Mohammad Yousef Alikhani Ali Mojtahedi |
| author_sort | Bahareh Lashtoo Aghaee |
| collection | DOAJ |
| description | Abstract Background P. aeruginosa is the primary source of hospital-acquired infections. Unfortunately, antibiotic resistance is growing to precariously high levels, making the infections by this pathogen life-threatening and hard to cure. One possible alternative to antibiotics is to use phages. However, the isolation of phages suitable for phage therapy— be lytic, be efficient, and have a broad host range —against some target bacteria has proven difficult. To identify the best places to look for these phages against P. aeruginosa we screened hospital sewages, soils, and rivers in two cities. Results We isolated eighteen different phages, determined their host range, infection property, and plaque morphology. We found that the sewage and sewage-contaminated environments are the most reliable sources for the isolation of Pseudomonas phages. In addition, phages isolated from hospital sewage showed the highest efficiency in lysing the bacteria used for host range determination. In contrast, phages from the river had larger plaque size and lysed bacteria with higher levels of antibiotic resistance. Conclusions Our findings provided additional support for the importance of sewage as the source of phage isolation. |
| first_indexed | 2024-12-15T00:13:57Z |
| format | Article |
| id | doaj.art-0c8980347bd84650a55f6ef1686cd9b7 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-15T00:13:57Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-0c8980347bd84650a55f6ef1686cd9b72022-12-21T22:42:29ZengBMCBMC Microbiology1471-21802021-05-012111810.1186/s12866-021-02197-zSewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosaBahareh Lashtoo Aghaee0Mohammadali Khan Mirzaei1Mohammad Yousef Alikhani2Ali Mojtahedi3Department of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesInstitute of Virology, Helmholtz Center Munich and Technical University of MunichDepartment of Microbiology, Faculty of Medicine, Hamadan University of Medical SciencesDepartment of Microbiology, School of Medicine, Guilan University of Medical SciencesAbstract Background P. aeruginosa is the primary source of hospital-acquired infections. Unfortunately, antibiotic resistance is growing to precariously high levels, making the infections by this pathogen life-threatening and hard to cure. One possible alternative to antibiotics is to use phages. However, the isolation of phages suitable for phage therapy— be lytic, be efficient, and have a broad host range —against some target bacteria has proven difficult. To identify the best places to look for these phages against P. aeruginosa we screened hospital sewages, soils, and rivers in two cities. Results We isolated eighteen different phages, determined their host range, infection property, and plaque morphology. We found that the sewage and sewage-contaminated environments are the most reliable sources for the isolation of Pseudomonas phages. In addition, phages isolated from hospital sewage showed the highest efficiency in lysing the bacteria used for host range determination. In contrast, phages from the river had larger plaque size and lysed bacteria with higher levels of antibiotic resistance. Conclusions Our findings provided additional support for the importance of sewage as the source of phage isolation.https://doi.org/10.1186/s12866-021-02197-zPseudomonas aeruginosaAntibiotic resistancePhage isolationPhage therapyHost-range |
| spellingShingle | Bahareh Lashtoo Aghaee Mohammadali Khan Mirzaei Mohammad Yousef Alikhani Ali Mojtahedi Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa BMC Microbiology Pseudomonas aeruginosa Antibiotic resistance Phage isolation Phage therapy Host-range |
| title | Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa |
| title_full | Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa |
| title_fullStr | Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa |
| title_full_unstemmed | Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa |
| title_short | Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa |
| title_sort | sewage and sewage contaminated environments are the most prominent sources to isolate phages against pseudomonas aeruginosa |
| topic | Pseudomonas aeruginosa Antibiotic resistance Phage isolation Phage therapy Host-range |
| url | https://doi.org/10.1186/s12866-021-02197-z |
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