Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis
Circular RNAs (circRNAs) characterize a novel kind of regulatory RNAs distinguished by great evolutionary conservation and constancy. Although their exact role in malignancies is not fully understood, they mainly work through specific axes. Circular RNA/miRNA/mRNA axes affect the pathogenesis of hum...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-06-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054024000167 |
| _version_ | 1827265208257609728 |
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| author | Maryam Abtin Nahid Nafisi Asghar Hosseinzadeh Sepideh Kadkhoda Ramesh Omranipour Leyla Sahebi Masoumeh Razipour Soudeh Ghafouri-Fard Abbas Shakoori |
| author_facet | Maryam Abtin Nahid Nafisi Asghar Hosseinzadeh Sepideh Kadkhoda Ramesh Omranipour Leyla Sahebi Masoumeh Razipour Soudeh Ghafouri-Fard Abbas Shakoori |
| author_sort | Maryam Abtin |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) characterize a novel kind of regulatory RNAs distinguished by great evolutionary conservation and constancy. Although their exact role in malignancies is not fully understood, they mainly work through specific axes. Circular RNA/miRNA/mRNA axes affect the pathogenesis of human cancers including breast cancer. We assessed the expression and function of circ_0009910/miR-145-5p/MUC1 axis in Breast Cancer tissues and MCF-7 cells. Expression levels of circ_0009910 and MUC1 were notably increased in breast cancer tissues compared with control tissues, parallel with the down-regulation of miR-145-5p. Clinicopathological analysis indicated that up-regulation of circ_0009910 in breast tumors is related to invasion of the tumor to lymph node (P value = 0.011). Also, the downregulation of miR-145-5p was significantly correlated with tumor invasion to lymph nodes (P value = 0.04) and HER2-negative tumors (P value = 0.037). Finally, overexpression of MUC1 was correlated with age under 45 years (P value = 0.002). More importantly, circ_0009910-siRNA decreased the proliferation and migration ability of breast cancer cells, enhanced expression of miR-145-5p, and decreased levels of MUC1. Taken together, the circ_0009910/miR-145-5p/MUC1 axis has been demonstrated to affect the pathogenesis of breast cancer and might provide a target for breast cancer treatment. |
| first_indexed | 2024-04-24T16:25:20Z |
| format | Article |
| id | doaj.art-0c8ba7fb37e247c7a34dad53b7e3212f |
| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2025-03-22T03:58:18Z |
| publishDate | 2024-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj.art-0c8ba7fb37e247c7a34dad53b7e3212f2024-04-28T10:42:13ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402024-06-0192367375Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axisMaryam Abtin0Nahid Nafisi1Asghar Hosseinzadeh2Sepideh Kadkhoda3Ramesh Omranipour4Leyla Sahebi5Masoumeh Razipour6Soudeh Ghafouri-Fard7Abbas Shakoori8Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranSurgery Department, Rasoul Akram Hospital Clinical Research Development Center (RCRDC), Iran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranBreast Disease Research Center (BDRC), Tehran University of Medical Sciences, Tehran, Iran; Department of Surgical Oncology, Cancer Institute, Tehran University of Medical Sciences, Tehran, IranMaternal, Fetal and Neonatal Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author.Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Medical Genetics, Cancer Institute of Iran, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Dr. Qarib St., Keshavarz Blvd, Tehran, Iran; Corresponding author. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Circular RNAs (circRNAs) characterize a novel kind of regulatory RNAs distinguished by great evolutionary conservation and constancy. Although their exact role in malignancies is not fully understood, they mainly work through specific axes. Circular RNA/miRNA/mRNA axes affect the pathogenesis of human cancers including breast cancer. We assessed the expression and function of circ_0009910/miR-145-5p/MUC1 axis in Breast Cancer tissues and MCF-7 cells. Expression levels of circ_0009910 and MUC1 were notably increased in breast cancer tissues compared with control tissues, parallel with the down-regulation of miR-145-5p. Clinicopathological analysis indicated that up-regulation of circ_0009910 in breast tumors is related to invasion of the tumor to lymph node (P value = 0.011). Also, the downregulation of miR-145-5p was significantly correlated with tumor invasion to lymph nodes (P value = 0.04) and HER2-negative tumors (P value = 0.037). Finally, overexpression of MUC1 was correlated with age under 45 years (P value = 0.002). More importantly, circ_0009910-siRNA decreased the proliferation and migration ability of breast cancer cells, enhanced expression of miR-145-5p, and decreased levels of MUC1. Taken together, the circ_0009910/miR-145-5p/MUC1 axis has been demonstrated to affect the pathogenesis of breast cancer and might provide a target for breast cancer treatment.http://www.sciencedirect.com/science/article/pii/S2468054024000167Breast cancerCirc_0009910miR-145-5pMUC1 |
| spellingShingle | Maryam Abtin Nahid Nafisi Asghar Hosseinzadeh Sepideh Kadkhoda Ramesh Omranipour Leyla Sahebi Masoumeh Razipour Soudeh Ghafouri-Fard Abbas Shakoori Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis Non-coding RNA Research Breast cancer Circ_0009910 miR-145-5p MUC1 |
| title | Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis |
| title_full | Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis |
| title_fullStr | Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis |
| title_full_unstemmed | Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis |
| title_short | Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis |
| title_sort | inhibition of breast cancer cell growth and migration through sirna mediated modulation of circ 0009910 mir 145 5p muc1 axis |
| topic | Breast cancer Circ_0009910 miR-145-5p MUC1 |
| url | http://www.sciencedirect.com/science/article/pii/S2468054024000167 |
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