Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies Vaccine
The present study is aimed to evaluate the effect of glycerol monolaurate (GML) on the growth performance and immune enhancement of pseudorabies virus (PRV)-inactivated vaccine in the early-weaned piglets. One hundred and twenty-five 28-day-old weaned piglets were randomly assigned to a control grou...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.891157/full |
| _version_ | 1798033820443738112 |
|---|---|
| author | Qinghai Ren Qinghai Ren Qinghai Ren Qinghai Ren Xiaobo Wang Xiaobo Wang Xiaobo Wang Qingqing Gao Qingqing Gao Qingqing Gao Gaiqin Wang Xiaochen Chen Chunxue Liu Song Gao Song Gao Song Gao Yubao Li |
| author_facet | Qinghai Ren Qinghai Ren Qinghai Ren Qinghai Ren Xiaobo Wang Xiaobo Wang Xiaobo Wang Qingqing Gao Qingqing Gao Qingqing Gao Gaiqin Wang Xiaochen Chen Chunxue Liu Song Gao Song Gao Song Gao Yubao Li |
| author_sort | Qinghai Ren |
| collection | DOAJ |
| description | The present study is aimed to evaluate the effect of glycerol monolaurate (GML) on the growth performance and immune enhancement of pseudorabies virus (PRV)-inactivated vaccine in the early-weaned piglets. One hundred and twenty-five 28-day-old weaned piglets were randomly assigned to a control group (CON, no vaccine and no challenge), challenge control group (C-CON), inactivated PRV vaccine group (IPV), IPV + 500 mg/kg GML group (L-GML), and IPV + 1,000 mg/kg GML group (H-GML) during the entire 28-day experimental period. All the data analyses were performed by one-way analysis of variance (ANOVA) and multiple comparisons. Our results showed that the final weight, average daily gain (ADG), and average daily feed intake (ADFI) of H-GML were the highest in each group, and F/G of H-GML was increased but there was no significant difference with CON (p > 0.05). Levels of PRV glycoprotein B (gB) antibody and immunoglobulin in serum of L-GML and H-GML were higher than those of IPV, but only gB antibody levels and immunoglobulin G (IgG) in H-GML were significantly increased (p < 0.05). Compared with IPV, the contents of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in serum of L-GML (TNF-α and IL-1β: p > 0.05, IL-6: p < 0.05, respectively) and H-GML (p < 0.01, both) were all decreased, and the content of interleukin-10 (IL-10) in H-GML was increased (p > 0.05). Furthermore, reverse transcription-polymerase chain reaction (RT-PCR) experiments proved that L-GML and H-GML were both superior to IPV in inhibiting the expression of TNF-α (p < 0.01), IL-6 (p > 0.05), and IL-1β (p < 0.01) mRNAs and promoting the expression of IL-10 mRNA (L-GML: p > 0.05, H-GML: p < 0.05, respectively) in the superficial inguinal lymph nodes. Histopathological examination found mild congestion in the lung and inguinal lymph nodes of IPV, while the tissues (brain, lung, and inguinal lymph nodes) of L-GML and H-GML were the same as CON with no obvious lesions. The above results indicate that GML may improve the growth performance of weaned piglets and enhance the immunity of PRV-inactivated vaccine by increasing the levels of PRV gB antibody and immunoglobulin and regulating cytokine levels. |
| first_indexed | 2024-04-11T20:34:51Z |
| format | Article |
| id | doaj.art-0c8c23bf918d47449abc4e5f5893cd0a |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T20:34:51Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-0c8c23bf918d47449abc4e5f5893cd0a2022-12-22T04:04:23ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-09-01910.3389/fvets.2022.891157891157Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies VaccineQinghai Ren0Qinghai Ren1Qinghai Ren2Qinghai Ren3Xiaobo Wang4Xiaobo Wang5Xiaobo Wang6Qingqing Gao7Qingqing Gao8Qingqing Gao9Gaiqin Wang10Xiaochen Chen11Chunxue Liu12Song Gao13Song Gao14Song Gao15Yubao Li16College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaAnyou Biotechnology Group Co., Ltd., Taicang, ChinaAnyou Biotechnology Group Co., Ltd., Taicang, ChinaAnyou Biotechnology Group Co., Ltd., Taicang, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture, Yangzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaInstitutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, ChinaCollege of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng, ChinaThe present study is aimed to evaluate the effect of glycerol monolaurate (GML) on the growth performance and immune enhancement of pseudorabies virus (PRV)-inactivated vaccine in the early-weaned piglets. One hundred and twenty-five 28-day-old weaned piglets were randomly assigned to a control group (CON, no vaccine and no challenge), challenge control group (C-CON), inactivated PRV vaccine group (IPV), IPV + 500 mg/kg GML group (L-GML), and IPV + 1,000 mg/kg GML group (H-GML) during the entire 28-day experimental period. All the data analyses were performed by one-way analysis of variance (ANOVA) and multiple comparisons. Our results showed that the final weight, average daily gain (ADG), and average daily feed intake (ADFI) of H-GML were the highest in each group, and F/G of H-GML was increased but there was no significant difference with CON (p > 0.05). Levels of PRV glycoprotein B (gB) antibody and immunoglobulin in serum of L-GML and H-GML were higher than those of IPV, but only gB antibody levels and immunoglobulin G (IgG) in H-GML were significantly increased (p < 0.05). Compared with IPV, the contents of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in serum of L-GML (TNF-α and IL-1β: p > 0.05, IL-6: p < 0.05, respectively) and H-GML (p < 0.01, both) were all decreased, and the content of interleukin-10 (IL-10) in H-GML was increased (p > 0.05). Furthermore, reverse transcription-polymerase chain reaction (RT-PCR) experiments proved that L-GML and H-GML were both superior to IPV in inhibiting the expression of TNF-α (p < 0.01), IL-6 (p > 0.05), and IL-1β (p < 0.01) mRNAs and promoting the expression of IL-10 mRNA (L-GML: p > 0.05, H-GML: p < 0.05, respectively) in the superficial inguinal lymph nodes. Histopathological examination found mild congestion in the lung and inguinal lymph nodes of IPV, while the tissues (brain, lung, and inguinal lymph nodes) of L-GML and H-GML were the same as CON with no obvious lesions. The above results indicate that GML may improve the growth performance of weaned piglets and enhance the immunity of PRV-inactivated vaccine by increasing the levels of PRV gB antibody and immunoglobulin and regulating cytokine levels.https://www.frontiersin.org/articles/10.3389/fvets.2022.891157/fullglycerol monolauratepseudorabies virusinactivated vaccineimmune enhancementweaned piglets |
| spellingShingle | Qinghai Ren Qinghai Ren Qinghai Ren Qinghai Ren Xiaobo Wang Xiaobo Wang Xiaobo Wang Qingqing Gao Qingqing Gao Qingqing Gao Gaiqin Wang Xiaochen Chen Chunxue Liu Song Gao Song Gao Song Gao Yubao Li Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies Vaccine Frontiers in Veterinary Science glycerol monolaurate pseudorabies virus inactivated vaccine immune enhancement weaned piglets |
| title | Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies Vaccine |
| title_full | Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies Vaccine |
| title_fullStr | Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies Vaccine |
| title_full_unstemmed | Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies Vaccine |
| title_short | Glycerol Monolaurate to Ameliorate Efficacy of Inactivated Pseudorabies Vaccine |
| title_sort | glycerol monolaurate to ameliorate efficacy of inactivated pseudorabies vaccine |
| topic | glycerol monolaurate pseudorabies virus inactivated vaccine immune enhancement weaned piglets |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.891157/full |
| work_keys_str_mv | AT qinghairen glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT qinghairen glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT qinghairen glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT qinghairen glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT xiaobowang glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT xiaobowang glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT xiaobowang glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT qingqinggao glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT qingqinggao glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT qingqinggao glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT gaiqinwang glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT xiaochenchen glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT chunxueliu glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT songgao glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT songgao glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT songgao glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine AT yubaoli glycerolmonolauratetoameliorateefficacyofinactivatedpseudorabiesvaccine |