Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with Nuruk
Vigeo is a mixture of fermented extracts of <i>Eleutherococcus senticosus</i> Maxim (ESM), <i>Achyranthes japonica</i> (Miq.) Nakai (AJN), and <i>Atractylodes japonica</i> Koidzumi (AJK) manufactured using the traditional Korean nuruk fermentation method. Although...
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MDPI AG
2021-10-01
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| author | So Young Eun Yoon-Hee Cheon Gyeong Do Park Chong Hyuk Chung Chang Hoon Lee Ju-Young Kim Myeung Su Lee |
| author_facet | So Young Eun Yoon-Hee Cheon Gyeong Do Park Chong Hyuk Chung Chang Hoon Lee Ju-Young Kim Myeung Su Lee |
| author_sort | So Young Eun |
| collection | DOAJ |
| description | Vigeo is a mixture of fermented extracts of <i>Eleutherococcus senticosus</i> Maxim (ESM), <i>Achyranthes japonica</i> (Miq.) Nakai (AJN), and <i>Atractylodes japonica</i> Koidzumi (AJK) manufactured using the traditional Korean nuruk fermentation method. Although the bioactive effects of ESM, AJN, and AJK have already been reported, the pharmacological effects of Vigeo have not been proven. Therefore, in this study, we investigated whether Vigeo had inhivitory effects on lipopolysaccharide (LPS)-induced inflammatory bone loss in vivo and receptor activator of nuclear factor-B ligand (RANKL)-induced osteoclastogenesis and the related mechanism in vitro. Vigeo administration conferred effective protection against bone loss induced by excessive inflammatory response and activity of osteoclasts in LPS-induced inflammatory osteoporosis mouse model. In addition, Vigeo significantly suppressed the formation of tartrate-resistant acid phosphatase-positive osteoclasts induced by RANKL and inhibited F-actin formation and bone resorbing activity without any cytotoxicity. Moreover, Vigeo significantly inhibited RANKL-induced phosphorylation of p38, ERK, JNK, IκB, and AKT and degradation of IkB. Additionally, Vigeo strongly inhibited the mRNA and protein expression of c-FOS and NFATc1 and subsequently attenuated the expression of osteoclast specific marker genes induced by RANKL. We demonstrated for the first time the anti-osteoporosis effect of Vigeo, suggesting that it could be a potential therapeutic candidate for the treatment of osteoclast-mediated inflammatory bone diseases. |
| first_indexed | 2024-03-10T05:11:33Z |
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| language | English |
| last_indexed | 2024-03-10T05:11:33Z |
| publishDate | 2021-10-01 |
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| series | Nutrients |
| spelling | doaj.art-0c90b92aa7024a71b9d360715e3b35242023-11-23T00:47:41ZengMDPI AGNutrients2072-66432021-10-011311390410.3390/nu13113904Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with NurukSo Young Eun0Yoon-Hee Cheon1Gyeong Do Park2Chong Hyuk Chung3Chang Hoon Lee4Ju-Young Kim5Myeung Su Lee6Musculoskeletal and Immune Disease Research Institute, School of Medicine, Wonkwang University, 460 Iksandae-ro, Iksan 54538, KoreaMusculoskeletal and Immune Disease Research Institute, School of Medicine, Wonkwang University, 460 Iksandae-ro, Iksan 54538, KoreaMusculoskeletal and Immune Disease Research Institute, School of Medicine, Wonkwang University, 460 Iksandae-ro, Iksan 54538, KoreaMusculoskeletal and Immune Disease Research Institute, School of Medicine, Wonkwang University, 460 Iksandae-ro, Iksan 54538, KoreaMusculoskeletal and Immune Disease Research Institute, School of Medicine, Wonkwang University, 460 Iksandae-ro, Iksan 54538, KoreaMusculoskeletal and Immune Disease Research Institute, School of Medicine, Wonkwang University, 460 Iksandae-ro, Iksan 54538, KoreaMusculoskeletal and Immune Disease Research Institute, School of Medicine, Wonkwang University, 460 Iksandae-ro, Iksan 54538, KoreaVigeo is a mixture of fermented extracts of <i>Eleutherococcus senticosus</i> Maxim (ESM), <i>Achyranthes japonica</i> (Miq.) Nakai (AJN), and <i>Atractylodes japonica</i> Koidzumi (AJK) manufactured using the traditional Korean nuruk fermentation method. Although the bioactive effects of ESM, AJN, and AJK have already been reported, the pharmacological effects of Vigeo have not been proven. Therefore, in this study, we investigated whether Vigeo had inhivitory effects on lipopolysaccharide (LPS)-induced inflammatory bone loss in vivo and receptor activator of nuclear factor-B ligand (RANKL)-induced osteoclastogenesis and the related mechanism in vitro. Vigeo administration conferred effective protection against bone loss induced by excessive inflammatory response and activity of osteoclasts in LPS-induced inflammatory osteoporosis mouse model. In addition, Vigeo significantly suppressed the formation of tartrate-resistant acid phosphatase-positive osteoclasts induced by RANKL and inhibited F-actin formation and bone resorbing activity without any cytotoxicity. Moreover, Vigeo significantly inhibited RANKL-induced phosphorylation of p38, ERK, JNK, IκB, and AKT and degradation of IkB. Additionally, Vigeo strongly inhibited the mRNA and protein expression of c-FOS and NFATc1 and subsequently attenuated the expression of osteoclast specific marker genes induced by RANKL. We demonstrated for the first time the anti-osteoporosis effect of Vigeo, suggesting that it could be a potential therapeutic candidate for the treatment of osteoclast-mediated inflammatory bone diseases.https://www.mdpi.com/2072-6643/13/11/3904Vigeonuruk fermentationosteoclastsbone resorptionosteoporosis |
| spellingShingle | So Young Eun Yoon-Hee Cheon Gyeong Do Park Chong Hyuk Chung Chang Hoon Lee Ju-Young Kim Myeung Su Lee Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with Nuruk Nutrients Vigeo nuruk fermentation osteoclasts bone resorption osteoporosis |
| title | Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with Nuruk |
| title_full | Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with Nuruk |
| title_fullStr | Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with Nuruk |
| title_full_unstemmed | Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with Nuruk |
| title_short | Anti-Osteoporosis Effects of the <i>Eleutherococcus senticosus</i>, <i>Achyranthes japonica</i>, and <i>Atractylodes japonica</i> Mixed Extract Fermented with Nuruk |
| title_sort | anti osteoporosis effects of the i eleutherococcus senticosus i i achyranthes japonica i and i atractylodes japonica i mixed extract fermented with nuruk |
| topic | Vigeo nuruk fermentation osteoclasts bone resorption osteoporosis |
| url | https://www.mdpi.com/2072-6643/13/11/3904 |
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