The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste
This study presents the effect of hydrolysis on the antioxidant activity of olive mill waste. The olive pomace samples were collected at different stages of maturity and were investigated for their phenolic content and antioxidant activity. Three different extraction procedures were employed, includ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/23/12187 |
| _version_ | 1797463590428475392 |
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| author | Karen Attard Mecit Halil Oztop Frederick Lia |
| author_facet | Karen Attard Mecit Halil Oztop Frederick Lia |
| author_sort | Karen Attard |
| collection | DOAJ |
| description | This study presents the effect of hydrolysis on the antioxidant activity of olive mill waste. The olive pomace samples were collected at different stages of maturity and were investigated for their phenolic content and antioxidant activity. Three different extraction procedures were employed, including methanolic maceration extraction and two hydrolysed procedures using 6 M HCL for acid hydrolysis and 10 M NaOH for alkaline hydrolysis. The total phenolic, flavonoid and <i>ortho</i>-diphenolic content, metal ion reducing activity, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-Diphenyl-1-picrylhydrazyl <i>Radical Scavenging</i>, hydrogen peroxide and <i>superoxide scavenging</i> activity assays were determined for the different extracts. In this study, cultivar and maturation of olives was one of the factors that affected the phenolic content in the olive pomace samples. Results show that alkaline hydrolysis had the highest antioxidant activity with respect to total phenolic content, 2,2-Diphenyl-1-picrylhydrazyl scavenging activity, metal ion reducing activity and <i>superoxide scavenging</i> activity, whereas acid hydrolysis had the highest 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity. The correlation analysis carried out on the different phenolic classes revealed that the total phenolic, flavonoid and <i>ortho</i>-diphenolic content were correlated with metal ion reducing activity and <i>Radical Scavenging</i> activity. |
| first_indexed | 2024-03-09T17:52:54Z |
| format | Article |
| id | doaj.art-0c93c23e38ff4c58a57f999ebe19e8ec |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T17:52:54Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-0c93c23e38ff4c58a57f999ebe19e8ec2023-11-24T10:32:23ZengMDPI AGApplied Sciences2076-34172022-11-0112231218710.3390/app122312187The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill WasteKaren Attard0Mecit Halil Oztop1Frederick Lia2Institute of Applied Science, Malta College of Arts, Science and Technology, PLA 9032 Paola, MaltaDepartment of Food Engineering, Middle East Technical University, Ankara 06800, TurkeyInstitute of Applied Science, Malta College of Arts, Science and Technology, PLA 9032 Paola, MaltaThis study presents the effect of hydrolysis on the antioxidant activity of olive mill waste. The olive pomace samples were collected at different stages of maturity and were investigated for their phenolic content and antioxidant activity. Three different extraction procedures were employed, including methanolic maceration extraction and two hydrolysed procedures using 6 M HCL for acid hydrolysis and 10 M NaOH for alkaline hydrolysis. The total phenolic, flavonoid and <i>ortho</i>-diphenolic content, metal ion reducing activity, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-Diphenyl-1-picrylhydrazyl <i>Radical Scavenging</i>, hydrogen peroxide and <i>superoxide scavenging</i> activity assays were determined for the different extracts. In this study, cultivar and maturation of olives was one of the factors that affected the phenolic content in the olive pomace samples. Results show that alkaline hydrolysis had the highest antioxidant activity with respect to total phenolic content, 2,2-Diphenyl-1-picrylhydrazyl scavenging activity, metal ion reducing activity and <i>superoxide scavenging</i> activity, whereas acid hydrolysis had the highest 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity. The correlation analysis carried out on the different phenolic classes revealed that the total phenolic, flavonoid and <i>ortho</i>-diphenolic content were correlated with metal ion reducing activity and <i>Radical Scavenging</i> activity.https://www.mdpi.com/2076-3417/12/23/12187antioxidantsphenolic contentolive pomacehydrolysis |
| spellingShingle | Karen Attard Mecit Halil Oztop Frederick Lia The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste Applied Sciences antioxidants phenolic content olive pomace hydrolysis |
| title | The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste |
| title_full | The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste |
| title_fullStr | The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste |
| title_full_unstemmed | The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste |
| title_short | The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste |
| title_sort | effect of hydrolysis on the antioxidant activity of olive mill waste |
| topic | antioxidants phenolic content olive pomace hydrolysis |
| url | https://www.mdpi.com/2076-3417/12/23/12187 |
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