Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it>
<p>Abstract</p> <p>We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga <it>Chlamydomonas reinhardtii</it>. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor...
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| Format: | Article |
| Language: | English |
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BMC
2011-11-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | http://www.plantmethods.com/content/7/1/35 |
| _version_ | 1831601343125520384 |
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| author | Strenkert Daniela Schmollinger Stefan Schroda Michael |
| author_facet | Strenkert Daniela Schmollinger Stefan Schroda Michael |
| author_sort | Strenkert Daniela |
| collection | DOAJ |
| description | <p>Abstract</p> <p>We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga <it>Chlamydomonas reinhardtii</it>. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.</p> |
| first_indexed | 2024-12-18T15:01:42Z |
| format | Article |
| id | doaj.art-0c96f4d932fb44bcb1d8195c6b666640 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-12-18T15:01:42Z |
| publishDate | 2011-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-0c96f4d932fb44bcb1d8195c6b6666402022-12-21T21:03:53ZengBMCPlant Methods1746-48112011-11-01713510.1186/1746-4811-7-35Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it>Strenkert DanielaSchmollinger StefanSchroda Michael<p>Abstract</p> <p>We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga <it>Chlamydomonas reinhardtii</it>. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.</p>http://www.plantmethods.com/content/7/1/35Chromatin immunoprecipitation<it>Chlamydomonas reinhardtii</it>heat shockformaldehyde crosslinkingreal-time PCRnucleosome occupancyhistone modification<it>HSP70A</it><it>RBCS2</it><it>CYC6</it> |
| spellingShingle | Strenkert Daniela Schmollinger Stefan Schroda Michael Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it> Plant Methods Chromatin immunoprecipitation <it>Chlamydomonas reinhardtii</it> heat shock formaldehyde crosslinking real-time PCR nucleosome occupancy histone modification <it>HSP70A</it> <it>RBCS2</it> <it>CYC6</it> |
| title | Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it> |
| title_full | Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it> |
| title_fullStr | Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it> |
| title_full_unstemmed | Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it> |
| title_short | Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it> |
| title_sort | protocol methodology for chromatin immunoprecipitation chip in it chlamydomonas reinhardtii it |
| topic | Chromatin immunoprecipitation <it>Chlamydomonas reinhardtii</it> heat shock formaldehyde crosslinking real-time PCR nucleosome occupancy histone modification <it>HSP70A</it> <it>RBCS2</it> <it>CYC6</it> |
| url | http://www.plantmethods.com/content/7/1/35 |
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