Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it>

Protocol: methodology for chromatin immunoprecipitation (ChIP) in <it>Chlamydomonas reinhardtii</it>

<p>Abstract</p> <p>We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga <it>Chlamydomonas reinhardtii</it>. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor...

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Bibliographic Details
Main Authors: Strenkert Daniela, Schmollinger Stefan, Schroda Michael
Format: Article
Language:English
Published: BMC 2011-11-01
Series:Plant Methods
Subjects:
Chromatin immunoprecipitation
<it>Chlamydomonas reinhardtii</it>
heat shock
formaldehyde crosslinking
real-time PCR
nucleosome occupancy
histone modification
<it>HSP70A</it>
<it>RBCS2</it>
<it>CYC6</it>
Online Access:http://www.plantmethods.com/content/7/1/35

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