Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health
Cellulomonas sp. HM71, a human gut microbe possesses metabolic machinery to catabolize antigenic gluten, hence, holds promises as microbial therapy to treat gluten-derived celiac disease. However, its efficacy, safety, and survivability in the gastrointestinal ecosystem await functional elucidation....
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1082674/full |
| _version_ | 1797953996320669696 |
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| author | Monika Yadav Tarun Kumar Ranjeet Maurya Ranjeet Maurya Rajesh Pandey Rajesh Pandey Nar Singh Chauhan |
| author_facet | Monika Yadav Tarun Kumar Ranjeet Maurya Ranjeet Maurya Rajesh Pandey Rajesh Pandey Nar Singh Chauhan |
| author_sort | Monika Yadav |
| collection | DOAJ |
| description | Cellulomonas sp. HM71, a human gut microbe possesses metabolic machinery to catabolize antigenic gluten, hence, holds promises as microbial therapy to treat gluten-derived celiac disease. However, its efficacy, safety, and survivability in the gastrointestinal ecosystem await functional elucidation. The current study is designed to characterize Cellulomonas sp. HM71 for its physiological, genomic, and probiotic properties. The morphological and physiological assessment indicates it as a coccus-shaped gram-positive bacterium growing optimally at 30°C in a neutral environment (pH 7.0). Cellulomonas sp. HM71 showed continuous growth even in stressful environments (salinity up to 3% NaCl and 6% KCl), variable temperature (25°C to 35°C) and pH (5-9), antibiotics, and gastric and intestinal conditions. The Cellulomonas sp. HM71 genome harbors diversified genetic machinery to modulate humongous metabolic potential for the host. This was substantiated by the hemolytic and CaCo-2 cell line assay which confirms its cellular adherence and biosafety. Notably, genome analysis did not identify any pathogenic islands. Probiotic characterization indicates its potential to overcome waterborne infections and digestion-related disorders. Cumulatively, Cellulomonas sp. HM71 can be considered a probiotic strain for improving human health because of the highlighted functions. |
| first_indexed | 2024-04-10T23:11:51Z |
| format | Article |
| id | doaj.art-0c9a6de5d4e74348b6f2e4cead0092e5 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T23:11:51Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-0c9a6de5d4e74348b6f2e4cead0092e52023-01-13T05:12:14ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011210.3389/fcimb.2022.10826741082674Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human healthMonika Yadav0Tarun Kumar1Ranjeet Maurya2Ranjeet Maurya3Rajesh Pandey4Rajesh Pandey5Nar Singh Chauhan6Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, IndiaDepartment of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, IndiaINtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, Division of Immunology and Infectious Diseases, Council of Scientific & Industrial Research-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaINtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, Division of Immunology and Infectious Diseases, Council of Scientific & Industrial Research-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), Ghaziabad, IndiaDepartment of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, IndiaCellulomonas sp. HM71, a human gut microbe possesses metabolic machinery to catabolize antigenic gluten, hence, holds promises as microbial therapy to treat gluten-derived celiac disease. However, its efficacy, safety, and survivability in the gastrointestinal ecosystem await functional elucidation. The current study is designed to characterize Cellulomonas sp. HM71 for its physiological, genomic, and probiotic properties. The morphological and physiological assessment indicates it as a coccus-shaped gram-positive bacterium growing optimally at 30°C in a neutral environment (pH 7.0). Cellulomonas sp. HM71 showed continuous growth even in stressful environments (salinity up to 3% NaCl and 6% KCl), variable temperature (25°C to 35°C) and pH (5-9), antibiotics, and gastric and intestinal conditions. The Cellulomonas sp. HM71 genome harbors diversified genetic machinery to modulate humongous metabolic potential for the host. This was substantiated by the hemolytic and CaCo-2 cell line assay which confirms its cellular adherence and biosafety. Notably, genome analysis did not identify any pathogenic islands. Probiotic characterization indicates its potential to overcome waterborne infections and digestion-related disorders. Cumulatively, Cellulomonas sp. HM71 can be considered a probiotic strain for improving human health because of the highlighted functions.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1082674/fullhuman gut microbiotaprobioticsmicrobial augmentationmicrobiome therapygluten metabolismprolyl endopeptidase |
| spellingShingle | Monika Yadav Tarun Kumar Ranjeet Maurya Ranjeet Maurya Rajesh Pandey Rajesh Pandey Nar Singh Chauhan Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health Frontiers in Cellular and Infection Microbiology human gut microbiota probiotics microbial augmentation microbiome therapy gluten metabolism prolyl endopeptidase |
| title | Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health |
| title_full | Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health |
| title_fullStr | Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health |
| title_full_unstemmed | Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health |
| title_short | Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health |
| title_sort | characterization of cellulomonas sp hm71 as potential probiotic strain for human health |
| topic | human gut microbiota probiotics microbial augmentation microbiome therapy gluten metabolism prolyl endopeptidase |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1082674/full |
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